Shared Resources Core: Glycan Analysis & Synthesis

共享资源核心：聚糖分析

• 批准号: 8853916
• 负责人: Biswa Choudhury
• 金额: $ 23.86万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8853916
• 关键词: Bacteria; Biological; Biological Process; Catabolism; Cell surface; Cells; Complex; Coupled; Custom; Ensure; Epithelial Cells; GAG Gene; Glycosaminoglycans; Glycoside Hydrolases; Human; Hyaluronan; Immune; Individual; Inflammation; Inflammatory Response; Knowledge; Myeloid Cells; Oligosaccharides; Pan Genus; Play; Polysaccharides; Postdoctoral Fellow; Principal Investigator; Protocols documentation; Research Personnel; Resource Sharing; Resources; Sampling; Sialic Acids; Signal Transduction; Techniques; Training; Training Programs; Work; cell motility; cell type; experience; genetic analysis; graduate student; instrumentation; meetings; microbial; mimicry; molecular size; sialoadhesin; skills

OVERVIEW OF GLYCAN ANALYSIS COMPONENT: Glycans play key roles in biological processes relevant to inflammation, such as cell-cell recognition, cell migration, and signal transduction. In order to understand glycan function in inflammation, it is essential to have an in-depth knowledge of glycan structural diversity in various cell types. Because enormous structural diversity exists in naturally occurring glycans, it has proven difficult to devise a universal protocol for glycan analysis. Moreover, sample complexity makes the isolation and purification of cell surface glycans a major methodological challenge. The analytical component of Core B will work within the framework of the Glycotechnology Core Resource at UCSD, a well-known center for providing specialized glycan analysis (glycotech.ucsd.edu). The Glycotechnology Core Resource was established in 1993 and since then has provided analytical support to a large number of investigators. Over the years, the Resource has developed skills to isolate, purify and structurally characterize polysaccharides or oligosaccharides from samples with limited amounts of analyte in the presence of a large background of complex biological contaminants. The state-of-the-art instrumentation coupled with many years of analytical experience will ensure that the analytic needs of the proposed Projects will be met. Besides carrying out custom analyses, the Resource has also trained graduate students, postdoctoral fellows, and principal investigators in glycan analytical techniques. Thus, the analytical group of Core B will also participate In the training program described in Core A, as well as perform glycan analyses for the individual Projects. Table 1 describes analytical techniques that will be used by the Projects.

聚糖分析组件概述：聚糖在与炎症相关的生物过程中发挥着关键作用，例如细胞间识别、细胞迁移和信号转导。为了了解炎症中聚糖的功能，必须深入了解各种细胞类型中的聚糖结构多样性。由于天然存在的聚糖存在巨大的结构多样性，因此设计出通用的聚糖分析方案非常困难。此外，样品的复杂性使得细胞表面聚糖的分离和纯化成为主要的方法学挑战。 核心 B 的分析组件将在 UCSD 糖技术核心资源的框架内工作，该资源是提供专业聚糖分析的著名中心 (glutech.ucsd.edu)。糖技术核心资源成立于 1993 年，自那时起为大量研究人员提供了分析支持。多年来，该资源已开发出在存在大量复杂生物污染物的背景下从含有有限分析物的样品中分离、纯化多糖或寡糖并进行结构表征的技能。最先进的仪器加上多年的分析经验将确保满足拟议项目的分析需求。除了进行定制分析外，该资源还对研究生、博士后研究员和聚糖分析技术的主要研究人员进行了培训。因此，核心 B 的分析小组也将参加核心 A 中描述的培训计划，并对各个项目进行聚糖分析。表 1 描述了项目将使用的分析技术。