Metabolites from Edible Blue-Green Algae for Obesity-Induced Inflammation

可食用蓝绿藻的代谢物可治疗肥胖引起的炎症

基本信息

批准号：
8812586
负责人：
Marcy J Balunas
金额：
$ 45.38万
依托单位：
UNIVERSITY OF CONNECTICUT STORRS
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-01-01 至 2018-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8812586
关键词：
Adipocytes Adipose tissue Adverse effects Anti-Inflammatory Agents Anti-inflammatory Biological Biological Factors Bone Marrow Cardiovascular Diseases Carotene Carotenoids Chemicals Chronic Clinical Clinical Trials Complement Factor B Complementary and alternative medicine Consumption Country Cyanobacterium Development Disease Drug Kinetics Expenditure Fatty acid glycerol esters Food Future Health Health Benefit Human In Vitro Inflammation Inflammation Mediators Inflammatory Insulin Resistance Knowledge Link Lipids Liver diseases Metabolic Metabolic Diseases Molecular Mus Non-Insulin-Dependent Diabetes Mellitus Nuclear Nuclear Translocation Obesity Pathogenesis Prevention Production Property Spirulina preparation Standardization United Nations base chemical standardization combat cytokine dietary supplements efficacy testing in vivo Model insulin signaling macrophage monocyte non-alcoholic fatty liver obesity prevention pre-clinical programs public health relevance quality assurance

项目摘要

DESCRIPTION (provided by applicant): Chronic low-grade inflammation is causally linked to the pathogenesis of obesity-associated metabolic diseases, such as insulin resistance (IR), type 2 diabetes, cardiovascular disease and non-alcoholic fatty liver disease. Fat overload in the adipocyte triggers circulating monocyte recruitment into lipid-laden adipose tissue, which produces a large quantity of pro-inflammatory mediators. This perturbs insulin signaling in adipose tissue, eventually inducing systemic inflammation and IR. As such, both metabolic and inflammatory components largely contribute to the development of chronic low-grade inflammation in obesity. Complementary and alternative medicine (CAM) natural products that can effectively combat chronic inflammation with minimal adverse side-effects are much needed. Spirulina platensis (SP) is a blue-green alga (BGA) that has proven to be safe for human consumption. During the past several years, we have investigated anti-inflammatory properties of BGA extracts, and more specifically a SP organic extract (SPE) using both in vitro and in vivo models and have found substantial evidence for their potential in the prevention of obesity-induced inflammation. However, major gaps exist in our knowledge regarding the bioactive components from edible BGA, which hinders the standardization and broader utilization of these CAM natural products. Very little is known about the chemical composition of BGA. Currently, standardization utilizes -carotene, total carotenoids, and/or phycocyanin content for quality assurance. These components have not been shown to cause the potent anti-inflammatory activity of BGA and thus are not appropriate marker compounds for chemical standardization based on biological activity. The following specific aims are thus proposed: SA1, isolation and identification of anti-inflammatory compounds from SP extracts; and SA2, identification of molecular mechanisms responsible for anti-inflammatory effects of SP extracts and purified compounds. The identification of bioactive metabolites responsible for the anti-inflammatory effects of BGA will help to support future standardization of these CAM products and additional information regarding their mechanism of action will allow for further preclinical and clinical development.

描述（由申请人提供）：慢性低度炎症与肥胖相关的代谢疾病的发病机理有因果关系，例如胰岛素抵抗（IR），2型糖尿病，心血管疾病和非熟饮脂肪肝疾病。脂肪细胞中的脂肪超负荷触发循环单核细胞募集到脂质脂肪组织中，该组织产生大量的促炎性介质。这种渗透性胰岛素信号传导在脂肪组织中，最终诱导全身炎症和IR。因此，代谢和炎症成分都在很大程度上有助于肥胖症中慢性低级炎症的发展。急需对慢性炎症的互补和替代药物（CAM）天然产物非常需要对慢性炎症进行对抗。 Spirulina Platensis（SP）是一种蓝绿色藻类（BGA），被证明对人类消费是安全的。在过去的几年中，我们研究了BGA提取物的抗炎特性，更具体地说是使用体外和体内模型的SP有机提取物（SPE）（SPE），并为预防肥胖引起的炎症提供了大量证据。但是，我们的知识中存在有关食用BGA的生物活性成分的主要差距，这阻碍了这些CAM天然产物的标准化和更广泛的利用。关于BGA的化学成分知之甚少。目前，标准化利用 - 芳香烯，总类胡萝卜素和/或植物素含量来保证质量。这些成分尚未证明会引起BGA的有效抗炎活性，因此，基于生物学活性的化学标准化不是适当的标记化合物。因此提出了以下特定目的：SA1，SP提取物的抗炎化合物的分离和鉴定；和SA2，鉴定了负责SP提取物和纯化化合物抗炎作用的分子机制。鉴定负责BGA抗炎作用的生物活性代谢产物将有助于支持这些CAM产品的未来标准化以及有关其作用机制的其他信息，将允许进一步的临床前和临床发展。