Genetic Etiology of Agammaglobulinemia with Absent B Cells

B 细胞缺失的无丙种球蛋白血症的遗传病因学

• 批准号: 8860110
• 负责人: MARY ELLEN CONLEY
• 金额: $ 36.83万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-05 至 2015-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8860110
• 关键词: Agammaglobulinemia; Alternative Splicing; Amino Acid Substitution; Autoimmune Diseases; B cell differentiation; B-Cell Development; B-Lymphocytes; BHLH Protein; Base Pairing; Binding; Biological Assay; Bone Marrow; CD19 gene; Cell Cycle Regulation; Cell Line; Cell Lineage; Cell Nucleus; Cells; DNA; DNA Binding; DNA Sequence; Defect; Development; Dominant-Negative Mutation; Electrophoretic Mobility Shift Assay; Employee Strikes; Enhancers; Event; Family; Family history of; Gene Expression Profile; Genes; Genetic; Genetic Predisposition to Disease; Genome; Germ-Line Mutation; Health; Helix-Loop-Helix Motifs; Helix-Turn-Helix Motifs; Homo; Human Cell Line; Immune system; Immunologic Deficiency Syndromes; In Vitro; Injection of therapeutic agent; Knock-in Mouse; Knockout Mice; Laboratories; Luciferases; Lymphoid; Malignant Neoplasms; Mouse Strains; Mus; Mutant Strains Mice; Mutation; Nucleic Acid Regulatory Sequences; Parents; Pathway interactions; Patients; Phenotype; Proteins; Receptors, Antigen, B-Cell; Recombinants; Reporter; Reporting; Site; Somatic Mutation; Staging; System; TCF3 gene; Testing; Tissues; Transcriptional Regulation; Tyrosine Phosphorylation; V(D)J Recombination; Wild Type Mouse; blastocyst; cancer therapy; chromatin immunoprecipitation; consanguineous family; design; dimer; embryonic stem cell; exome sequencing; expression vector; helix-loop-helix protein E12; helix-loop-helix protein E47; homologous recombination; in vivo; interest; leukemia/lymphoma; member; mouse model; mutant; research study; targeted sequencing; transcription factor; transcriptome sequencing; vector

DESCRIPTION (provided by applicant): E2A is a broadly expressed transcription factor that is essential for early B cell development. The gene for E2A, TCF3, encodes 2 unique basic helix loop helix (bHLH) proteins, E12 and E47, by alternative splicing. Both E12 and E47 can function as homodimers and as heterodimers with each other or with tissue specific transcription factors. Although somatic mutations and translocations involving E2A are seen in leukemias and lymphomas, germline mutations in this gene have not been reported. We have recently identified 4 patients with markedly reduced numbers of B cells and agammaglobulinemia who have exactly the same de novo heterozygous amino acid substitution (E555K) in the basic region of the bHLH domain of E47, the DNA binding segment of the protein. The small number of B cells present in these patients have an unusual phenotype characterized by the increased expression of CD19, a downstream target of E2A, and the absence of a B cell antigen receptor. Bone marrow analysis shows reduced numbers of pro-B cells, increased expression of CD19 and increased tyrosine phosphorylation. Studies with expression vectors indicate that the mutant protein is stable and localizes to the nucleus. The mutation causes a dominant negative effect in electrophoretic mobility shift assays (EMSAs) and luciferase reporters when binding the murine m enhancer. To better understand events that control early B cell development, the proposed studies will examine the functional consequences of the E47 mutation. In Specific Aim 1, we will determine if the E555K mutation in E47 alters its ability to dimerize with essential partners or to bind critical DNA sequences as a homodimer or heterodimer using EMSAs, and chromatin immunoprecipitation (ChIP). The ChIP assays will be done using B cell lines representing various stages of B cell differentiation that have been transfected with E47 wild type or mutant expression vectors. In Specific Aim 2, we will create a knock-in strain of mice with the E555K mutation, and compare these mice with E2A-/- mice and E47-/- mice as well as wild type mice. The number and phenotype of B lineage cells in these mice, the VDJ recombination status and the expression of activation markers will be examined. In Specific Aim 3 we will analyze the functional consequences of mutant E47 in in vivo and in vitro systems. RNA-seq will be used to analyze the alterations in the transcriptome of B cell precursors from wild type versus mutant mice. Regulatory regions of genes identified by ChIP or RNA-seq will be verified by reporter contructs. Finally, transcriptome analysis of common lymphoid precursors from the patients will be performed. The identification of the E555K mutation represents the first autosomal dominant form of agammaglobulinemia. It conforms to recent observations indicating that de novo mutations are not rare and are likely to occur at specific sites in the genome. The results of the proposed studies will enhance our understanding of the requirements for early B cell development and may suggest pathways that be used to treat autoimmune disease and cancer.

描述（由申请人提供）：E2A是一种广泛表达的转录因子，对于早期B细胞发育至关重要。 E2A，TCF3的基因编码2种独特的碱性螺旋环螺旋（BHLH）蛋白E12和E47，通过替代剪接。 E12和E47都可以用作同二聚体，彼此或组织特定转录因子的异二聚体。尽管在白血病和淋巴瘤中可以看到涉及E2a的体细胞突变和易位，但尚未报道该基因中的种系突变。我们最近确定了4例B细胞数量明显减少的患者和Agammaglobulinemia在E47的BHLH结构域的基本区域中具有完全相同的杂合氨基酸取代（E555K）的患者。这些患者中存在的少量B细胞具有不寻常的表型，其特征是CD19的表达增加，E2A的下游靶标和不存在B细胞抗原受体的表现。骨髓分析表明，促进B细胞数量减少，CD19表达增加并增加酪氨酸磷酸化。用表达载体的研究表明，突变蛋白稳定并定位在细胞核上。在结合鼠M增强子时，该突变会在电泳迁移率转移测定（EMSA）和荧光素酶报告中引起主要负面影响。为了更好地了解控制早期B细胞发育的事件，拟议的研究将检查E47突变的功能后果。在特定的目标1中，我们将确定E47中的E555K突变是否会改变其与基本伴侣二聚体的能力，或使用EMSA和染色质免疫沉淀（CHIP）结合临界DNA序列作为同型二聚体或异二聚体的能力。 CHIP分析将使用代表B细胞分化的各个阶段的B细胞系进行，这些阶段已被E47野生型或突变体表达向量转染。在特定的目标2中，我们将使用E555K突变创建一只小鼠的敲击菌株，并将这些小鼠与E2A - / - 小鼠和E47 - / - 小鼠以及野生型小鼠进行比较。这些小鼠中B谱系细胞的数量和表型将检查VDJ重组状态和激活标记的表达。在特定目标3中，我们将分析突变E47在体内和体外系统中的功能后果。 RNA-seq将用于分析野生型与突变小鼠的B细胞前体转录组的改变。通过记者的发现，将通过芯片或RNA-seq鉴定的基因的调节区域进行验证。最后，将对患者的常见淋巴前体进行转录组分析。 E555K突变的鉴定代表了agammagloblobloblobilaine的第一种常染色体显性形式。它符合最近的观察结果，表明从头突变并不罕见，很可能发生在基因组的特定部位。拟议研究的结果将增强我们对早期B细胞发育需求的理解，并可能表明用于治疗自身免疫性疾病和癌症的途径。