Novel technologies to remove obscuring blood from fine needle aspiration biopsies

从细针抽吸活检中去除模糊血液的新技术

基本信息

批准号：
8905528
负责人：
Shrikumar Ambujakshan Nair
金额：
$ 69.81万
依托单位：
AFFINERGY, LLC
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-01 至 2018-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8905528
关键词：
Affinity Aspirate substance Binding Blood Blood Cells Cell Line Cells Chemistry Clinical Collecting Cell Cost Savings Cytology Data Development Diagnostic Diagnostic Procedure Diagnostic tests Equipment Erythrocytes Evaluation Fine needle aspiration biopsy Fluorescent in Situ Hybridization Funding Glass Hand Healthcare Systems Hemorrhage Housing Human Incidence Lead Leukocytes Life Liver Lung Malignant - descriptor Malignant Neoplasms Malignant neoplasm of thyroid Marketing Methods Operating Rooms Operative Surgical Procedures Oryctolagus cuniculus Outcome Pancreas Pathologist Patient Triage Patients Peptides Performance Phase Preparation Procedures Process Production Protocols documentation Quality of Care Recruitment Activity Reproducibility Sampling Side Site Slide Staining method Stains Surface Techniques Testing Thyroid Gland Thyroid Nodule Time United States Unnecessary Procedures Unnecessary Surgery Woman cell type clinically significant commercialization cost cost effective density immunocytochemistry improved men new technology novel prototype public health relevance satisfaction scale up screening

项目摘要

DESCRIPTION (provided by applicant): Fine-needle aspiration (FNA) cytology is the standard technique for evaluating thyroid nodules. Hemorrhaging is common during the procedure, however, leading to an aspirate that is significantly diluted by blood. Subsequent slide preparations are often suboptimal, resulting in nondiagnostic outcomes that necessitate additional testing or diagnostic surgery. In fact, quality is so unpredictable that a pathologist must often be present in the operating room to certify that the slides are adequate, thus adding substantial cost and complexity to the procedure. Ancillary testing such as immunocytochemistry and fluorescence in-situ hybridization can also be used for evaluating the aspirate, but such tests are not usually carried out using smears due to a paucity of follicular cells and an abundance of red blood cells. As a result, additional slides must be prepared using expensive and time-consuming techniques. Thus, there is a significant need for increasing the capture and isolation of thyroid cells on FNA slide preparations. Affinergy is developing a novel technology for improving the quality of thyroid FNA slide preparations. We have identified proprietary peptides that binds with high affinity to thyroid cells but not to red or white blood cells, and we have developed a cell-specific coating for glass slides that provides a simple, reproducible method for preparing optimal FNA slides from samples contaminated with blood. A reduction in non-diagnostic results will lead to improved quality of care and fewer unnecessary tests and diagnostic surgeries. At the conclusion of this Phase II project, we will have sufficient data in hand to rapidly bring our peptide-conjugated slides to market.

描述（由申请人提供）：精细的抽吸（FNA）细胞学是评估甲状腺结节的标准技术。，导致非诊断的结果需要进行测试或诊断手术。用于评估抽吸物，由于卵泡细胞的稀疏和丰富的红细胞，使用涂片进行的usua lly。增加甲状腺的捕获和分离在FNA幻灯片制剂上。载玻片的IC涂层提供了一种简单的再现方法，用于从样品中占用最佳的FNA玻片，这些幻灯片与血液污染了非诊断结果。有一份手头的数据迅速将我们的肽偶联幻灯片推向市场。