Upgrade of a FACS Aria Cell Sorter

FACS Aria 细胞分选仪的升级

• 批准号: 8826515
• 负责人: JONATHAN E BOYSON
• 金额: $ 15.3万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8826515
• 关键词: Advisory Committees; Cell Separation; Cells; Communities; Core Facility; Discipline; Faculty; Flow Cytometry; Funding; Grant; Housing; Lasers; Medicine; Research; Research Personnel; Scientist; Speed; United States National Institutes of Health; Universities; Vermont; college; cost; experience; instrument; meetings; red fluorescent protein; solid state

 DESCRIPTION (provided by applicant): A request is made for funds to install a Coherent 50 mW 561 nm solid-state laser and upgrade a FACS Aria high-speed cell sorter at the Flow Cytometry and Cell Sorting (FCCS) facility at the University of Vermont. The FCCS Core facility in the College of Medicine houses the only cell sorter present at the University. Therefore, this single instrument must be able to serve the needs of all investigators at the University who require cell sorting for their research. Currently, this instrument supports 15 NIH-supported investigators and 27 NIH grants and is serving a broad community of investigators from a variety of disciplines. Both shifting emphases in research and the recruitment of new faculty have resulted in a number of investigators who need to sort cells expressing red fluorescent proteins, which are optimally excited by a 561 nm laser line. Since our current instrument does not possess this laser, these investigators cannot conduct their NIH-funded research. We have determined that we can meet the needs of our investigators by upgrading our current instrument so that a 561 nm laser can be installed. This upgrade is a fraction of the cost of the purchase of a new instrument. The upgraded instrument will remain located in the FCCS core, which has been in existence for over 20 years. This facility is managed by an experienced and accredited scientist/cytometrist, is directed by a faculty advisor with over 20 years of experience in flow cytometry, and is overseen by an internal advisory committee. In addition, the facility continues to enjoy strong institutional support from the University Of Vermont College Of Medicine.

 描述（由应用程序提供）：要求资金安装连贯的50 mW 561 nm固态激光器，并在佛蒙特大学的流式细胞仪和细胞分配（FCCS）设施上升级FACS A FACS ARIA高速细胞分隔器。医学院的FCCS核心设施是该大学唯一出现的细胞分落者。因此，该单一的工具必须能够满足大学中所有需要细胞进行研究的调查人员的需求。目前，该工具支持15位由NIH支持的研究人员和27个NIH赠款，并为来自各种学科的研究人员提供服务。两种研究的重点和新教师的招募都导致许多研究者需要对表达红色荧光蛋白的细胞进行分类，这些细胞受到561 nm激光线的最佳激发。由于我们当前的工具不假定这种激光，因此这些研究人员无法进行NIH资助的研究。我们已经确定我们可以通过升级当前的仪器来满足调查人员的需求，从而可以安装561 nm激光器。此升级是购买新仪器成本的一小部分。升级的仪器将保留在FCCS核心中，该核心已经存在了20多年。该设施由经验丰富且经过认可的科学家/细胞术家管理，由具有20多年流式细胞仪经验的教师顾问指导，并由内部咨询委员会监督。此外，该设施继续享有佛蒙特大学医学院的强大机构支持。