Upgrade of a FACS Aria Cell Sorter

FACS Aria 细胞分选仪的升级

• 批准号: 8826515
• 负责人: JONATHAN E BOYSON
• 金额: $ 15.3万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8826515
• 关键词: Advisory Committees; Cell Separation; Cells; Communities; Core Facility; Discipline; Faculty; Flow Cytometry; Funding; Grant; Housing; Lasers; Medicine; Research; Research Personnel; Scientist; Speed; United States National Institutes of Health; Universities; Vermont; college; cost; experience; instrument; meetings; red fluorescent protein; solid state

 DESCRIPTION (provided by applicant): A request is made for funds to install a Coherent 50 mW 561 nm solid-state laser and upgrade a FACS Aria high-speed cell sorter at the Flow Cytometry and Cell Sorting (FCCS) facility at the University of Vermont. The FCCS Core facility in the College of Medicine houses the only cell sorter present at the University. Therefore, this single instrument must be able to serve the needs of all investigators at the University who require cell sorting for their research. Currently, this instrument supports 15 NIH-supported investigators and 27 NIH grants and is serving a broad community of investigators from a variety of disciplines. Both shifting emphases in research and the recruitment of new faculty have resulted in a number of investigators who need to sort cells expressing red fluorescent proteins, which are optimally excited by a 561 nm laser line. Since our current instrument does not possess this laser, these investigators cannot conduct their NIH-funded research. We have determined that we can meet the needs of our investigators by upgrading our current instrument so that a 561 nm laser can be installed. This upgrade is a fraction of the cost of the purchase of a new instrument. The upgraded instrument will remain located in the FCCS core, which has been in existence for over 20 years. This facility is managed by an experienced and accredited scientist/cytometrist, is directed by a faculty advisor with over 20 years of experience in flow cytometry, and is overseen by an internal advisory committee. In addition, the facility continues to enjoy strong institutional support from the University Of Vermont College Of Medicine.

 描述（通过应用程序提供）：在佛蒙特大学的流式细胞仪和细胞仪（FCC）设施中，要求资金安装50 MW 561 nm固态激光器和升级的咏叹调细胞分散者。 CERLTER的唯一能力能够满足大学的所有研究人员的需求，他们需要进行研究。两种研究的重点和新教师的募集都导致许多研究者需要对表达红色荧光蛋白的细胞进行分类，这些细胞在561 nm激光线上最佳地激发了。 Curreng Curreng Curreng Curreng Curreng Curreng Curreng Curreng Curreng Curreng Curreng Curreng仪器，因此可以安装561 NM激光。该设施的存在是由经验和细胞仪的顾问进行的。佛蒙特州医学学院的体学。