BioMark

生物标记

基本信息

批准号：
8446911
负责人：
Luc Teyton
金额：
$ 32.17万
依托单位：
SCRIPPS RESEARCH INSTITUTE, THE
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-07-01 至 2014-06-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Project Summary and Abstract This proposal by the Scripps Research Institute is aimed at purchasing a Biomark instrument from Fluidigm. This instrument will be dedicated to studying single cells at the transcriptional and respond to an increasing demand from the scientists of TSRI. The Institute is committed to fully support this application that stems from the Department of Immunology and Microbial Science. The necessary resources and personnel needed for operation will committed through housing the new instrument within the DNA Core of TSRI under the supervision of the Core director, Steven Head. Our request for a Biomark machine is motivated by the urgent need to bring to our institution a new technology that will allow experiments that cannot currently be carried out on-site and that will add to the quality of our output. This microfluidic machine has the huge advantage of versatility as it can be used for real-time PCR, digital PCR, genotyping, and sequencing. With respect to single cell analysis, we could not find comparable pieces of equipment from any of the competitive genomics hardware companies. In immunology, the need to study single cells has exploded over the past few years as the community has now realized of the heterogeneity of cell populations. This new way of looking at the immune response has been greatly facilitated by our capacity to isolate specific single B and T cells by fluorescence activated cell sorting using labeled antigens and labeled MHC multimers, respectively. The current hurdle is the limited ability that we have to extract multiparametric information from the isolated single cell. Similarly, neurobiologists want to interrogate single neurons and cancer biologist want to examine tumor heterogeneity. The instrument will be housed in and operated by the TSRI DNA Core Facility. For immunological applications the entire collection of primers will be available at cost to investigators. Indeed, te collection of primer sets needed for immunological applications is very standardized in both mouse and man and can be easily categorized. As a core instrument, the Biomark will be available to all investigators on campus under the supervision of the core director and its advisory committee. The core will be capable of providing basic training although formal manufacturer training will be highly encouraged before using the instrument. Booking will be on-line and under the supervision of the core director. The instrument will be under a maintenance contract paid by a cost-recovery program.

描述（由申请人提供）：项目概要和摘要斯克里普斯研究所的这项提案旨在从 Fluidigm 购买 Biomark 仪器。该仪器将致力于研究单细胞的转录，并响应 TSRI 科学家日益增长的需求。该研究所致力于全力支持这项源自免疫学和微生物科学系的应用。运营所需的必要资源和人员将通过将新仪器安置在 TSRI 的 DNA 核心内并在核心主任 Steven Head 的监督下进行承诺。我们对 Biomark 机器的需求是因为迫切需要为我们的机构带来一项新技术，该技术将允许目前无法在现场进行的实验，并提高我们的产出质量。该微流控机器具有多功能性的巨大优势，可用于实时PCR、数字PCR、基因分型和测序。在单细胞分析方面，我们无法从任何具有竞争力的基因组学硬件公司找到类似的设备。在免疫学中，随着社会现在意识到细胞群的异质性，研究单细胞的需求在过去几年中呈爆炸式增长。我们分别使用标记的抗原和标记的 MHC 多聚体通过荧光激活细胞分选来分离特定的单个 B 和 T 细胞的能力极大地促进了这种观察免疫反应的新方法。当前的障碍是我们从孤立的单细胞中提取多参数信息的能力有限。同样，神经生物学家想要询问单个神经元，癌症生物学家想要检查肿瘤异质性。该仪器将安装在 TSRI DNA 核心设施内并由 TSRI DNA 核心设施运行。对于免疫学应用，研究人员可以付费获得整个引物集。事实上，免疫学应用所需的引物组集合在小鼠和人类中都非常标准化，并且可以轻松分类。作为核心工具，Biomark 将在核心主任及其咨询委员会的监督下向校园内的所有研究人员开放。尽管强烈鼓励在使用仪器之前进行正式的制造商培训，但核心将能够提供基本培训。预订将在网上进行，并在核心总监的监督下进行。该仪器将签订维护合同，由成本回收计划支付费用。