An Ultra-fast Cooling Device for Vitrification and Cryopreservation of Cells and Tissues

用于细胞和组织玻璃化和冷冻保存的超快速冷却装置

• 批准号: 8834867
• 负责人: Xu Han
• 金额: $ 15.26万
• 依托单位: CRYOCRATE, LLC
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-15 至 2017-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8834867
• 关键词: Animal Model; Animals; Biochemical; Biological Testing; Biomedical Research; Blood Vessels; Cells; Cornea; Cryopreservation; Device Designs; Devices; Economics; Engineering; Excision; Family suidae; Film; Future; Genetic Engineering; Genotype; Goals; Heating; Histocompatibility Testing; Human; Infection; Investigation; Life; Liquid substance; Maintenance; Measures; Mechanics; Membrane; Metals; Methods; Nerve Tissue; Nitrogen; Operating System; Optics; Ovarian Tissue; Performance; Phase; Procedures; Production; Psychological reinforcement; Research Activity; Sampling; Skin; Skin Tissue; Solutions; Stainless Steel; Steel; Surface; System; Techniques; Technology; Testing; Thick; Time; Tissue Engineering; Tissue Sample; Tissue Viability; Tissues; Transplantation; Transportation; base; cell type; cryogenics; design; engineering design; evaporation; human tissue; improved; novel; prevent; prototype; public health relevance; seal; stem cell technology; vapor

DESCRIPTION (provided by applicant): Storage and maintenance of valuable genotypes of animal model species as live animal lines would be wholly impractical. However, traditional cryopreservation techniques will be soon found to be inefficient for providing economic approach for cryopreservation of cells and tissues from millions of new animal models being developed. Efficient cryopreservation of clinically valuable tissues, including cornea tissues, ovarian cortical strips, skins, blood vessels, and many others will invaluably benefit corresponding transplantation technology and basic biomedical research using animal model or human tissues. Our overall goal is to develop an ultra-fast cooling device (cooling rate >106 K/min) to achieve vitrification of thin (approximately 100-200 microns) tissues or samples without or with very low concentration of permeating cryoprotectants. We will achieve this by producing the prototype of an ultra-fast cooling device with an application of cutting-edge engineering techniques including cryogenic thin film evaporation and jet impingement cooling. To improve its practical efficiency, the device will be designed as a close operating system that prevents samples from direct contact with liquid nitrogen or its vapor. To achieve those goals, the plan for phase I periods is proposed: 1) design a novel ultra-fast cooling device combining thin film evaporation and jet impingement cooling techniques, determine optimal design parameters for subsystems; 2) produce the device and test its thermal efficiency; and 3) the tested device will be used for porcine and human corneal tissue cryopreservation, and the cryopreserved efficiency will be assessed according to biochemical and histological tests on the post-thaw tissues. At the end of Phase I, the proposed device will be produced under appropriate engineering design control and validated through biological tests.

描述（由申请人提供）：动物模型物种的宝贵基因型的存储和维护将完全不切实际。但是，很快就会发现，传统的冷冻保存技术将无效地提供经济方法来从开发数百万个新动物模型中对细胞和组织的冷冻保存和组织。有效地对临床上有价值的组织进行冷冻保存，包括角膜组织，卵巢皮层，皮肤，血管，血管等，将有必要使相应的移植技术和使用动物模型或人类组织的基本生物医学研究受益。我们的总体目标是开发一种超快速冷却装置（冷却速率> 106 k/min），以实现薄（大约100-200微米）组织的玻璃化或没有或以非常低浓度的渗透性冷冻保护剂的样品。我们将通过采用尖端工程技术（包括低温薄膜蒸发和喷气撞击冷却）的技术来实现这一目标。为了提高其实际效率，该设备将设计为近距离操作系统，可防止样品与液氮或蒸气直接接触。为了实现这些目标，提出了I期时期的计划：1）设计一种新型的超快速冷却装置，结合了薄膜蒸发和撞击撞击冷却技术，确定子系统的最佳设计参数； 2）生产设备并测试其热效率； 3）测试的装置将用于猪和人角膜组织冷冻保存，并且将根据对后诊所的生化和组织学测试评估冷冻保存的效率。在第一阶段结束时，将在适当的工程设计控制下生产提出的设备，并通过生物学测试进行验证。