Metabolomics Core

代谢组学核心

• 批准号: 8708030
• 负责人: GERALD I SHULMAN
• 金额: $ 20.61万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8708030
• 关键词: 1,2-diacylglycerol; Accounting; Acyl Coenzyme A; Address; Automation; Biochemistry; Biological Assay; Blood Plasma Volume; Calibration; Carbon; Ceramides; Charge; Chemistry; Communities; Complex; Complications of Diabetes Mellitus; Creatinine; Development; Diabetes Mellitus; Diabetic mouse; Diglycerides; Divalent Cations; Engineering; Ensure; Esters; Fatty Acids; Fee-for-Service Plans; Funding; Gene Expression; Glucose Plasma Concentration; Goals; High Pressure Liquid Chromatography; Hormones; Human Resources; Instruction; Isotopes; Kinetics; Laboratories; Length; Lipids; Liver Function Tests; Maintenance; Measurement; Measures; Metabolic; Metabolic Diseases; Methods; Modeling; Molecular; Mus; Nonesterified Fatty Acids; Obesity; Organ; Phenotype; Physiology; Plasma; Policies; Progress Reports; Protocols documentation; Quality Control; Radio; Renal clearance function; Research; Research Personnel; Resources; Sampling; Savings; Serum; Services; Stable Isotope Labeling; System; Systems Analysis; Testing; Tissue Sample; Tissues; Tracer; Training; Transgenic Mice; United States National Institutes of Health; Universities; Urine; Vendor; Washington; Work; base; carbohydrate metabolism; cytokine; design; enzyme activity; experience; flexibility; glucose metabolism; in vivo; instrument; instrumentation; lipid metabolism; liquid chromatography mass spectrometry; meetings; metabolomics; mouse model; programs; protein metabolism; research and development; research study; response; stable isotope

PROJECT SUMMARY (See instructions): The Metabolomics Core provides a facility to determine the phenotype of metabolite and hormone profiles of transgenic mouse models of diabetes and its complications. The services provided by the Metabolomics Core include the extraction, purification, derivatization, and instrumental analysis of metabolites, hormones, and selected enzyme activities from tissues and plasma. The methods have been scaled to account for the minimal amounts of tissues (-10 to 25 mg, and plasma volumes of - 5 to 25 pi) that can be provided by investigators.. The facility is equipped with an array of state-of-the-art instrumentation, including GC-MS, LC/MS/MS, HPLC, and NMR, providing the flexibility and resources for analyses of metabolite concentrations as well as sotopic enrichments. Metabolic panels of serum, plasma, and urine, including Chem 7, liver function tests lipid profiles, and divalent cations, are obtained using the COBAS MIRA system. We have optimized the COBAS Mira analytical system for analysis of mouse samples such that required volumes average -20 pi of serum for any combination of 4 tests. These plasma chemistry assays fill a need with very high demand for mouse studies, as reflected in our work flow over the last funding period. GC/MS and LC/MS/MS analyses provide complete profiles of chain length distribution of fatty acid metabolites, included free fatty acids, fatty acyl-CoA esters, diacylglycerols, ceramides. Other LC/MS/MS that are offered and are in high-demand include intermediates of glucose metabolism for metabolic flux measurements, and creatinines for renal clearance. The Metabolomics Core is a resource lab for the analysis of samples generated during the course of experiments of the Integrative Physiology Core at the Yale MMPC, and also provides the same array of assays to other outside investigators to assist in phenotyping of transgenic mouse models of diabetes Samples are analyzed on a fee-for-service basis with the charge per assay based on policy determined by the National MMPC Executive Committee, designed to provide substantial savings to NIH-funded users compared to commercial vendors. The Core has an active Research and Development Program to develop new analytical protocols to address new and exciting developments in the biochemistry of diabetes, obesity, and associated complications.

项目摘要（请参阅说明）： 代谢组学核心提供了一种设施，以确定糖尿病的转基因小鼠模型及其并发症的代谢物和激素谱的表型。代谢组核心提供的服务包括从组织和血浆中的代谢产物，激素和选定的酶活性的提取，纯化，衍生化和工具分析。这些方法已被缩放以说明研究人员可以提供的最小组织量（-10至25 mg，等离子体体积为-5至25 Pi）。 该设施配备了一系列最先进的仪器，包括GC-MS，LC/MS/MS，HPLC和NMR，提供了用于分析代谢物浓度的灵活性和资源，以及肌张力的富集。使用Cobas Mira系统获得血清，血浆和尿液的代谢面板，包括Chem 7，肝功能测试脂质谱和二价阳离子。我们已经优化了COBAS MIRA分析系统，以分析小鼠样品，以便为4个测试的任何组合所需的平均水平-20 Pi血清。这些等离子体化学分析满足了对小鼠研究的需求量很高的需求，这反映在我们上一个资金期间的工作流程中。 GC/MS和LC/MS/MS分析提供了脂肪酸代谢物的链长度分布的完整曲线，包括游离脂肪酸，脂肪酰基辅酶A酯，二酰基甘油，神经酰胺。提供并在高需求中提供的其他LC/MS/MS包括用于代谢通量测量的葡萄糖代谢中间体，以及用于肾脏清除率的肌酐。 代谢组学核心是一个资源实验室，用于分析YALE MMPC综合生理核心实验过程中生成的样品，并且还向其他外部研究人员提供了相同的测定方法，以根据持续的糖尿病的方式分析了基于糖尿病的实物，以促进委员会的委员会，从而在持续的委员会中分析了对糖尿病模型的表型，该委员会根据费用的委员会进行了委员会，该委员会根据费用的实质分析了委员与商业供应商相比，由NIH资助的用户致敬。核心有一个积极的研究和开发计划，可以开发新的分析方案，以解决糖尿病，肥胖和相关并发症的生物化学中新的令人兴奋的发展。