Transating Mussel Adhesion

平移贻贝粘附力

• 批准号: 8729438
• 负责人: JACOB N ISRAELACHVILI
• 金额: $ 41.29万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-04 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8729438
• 关键词: 3-Dimensional; Acids; Adhesions; Adhesives; Age; Binding; Collagen; Collagen Type I; Dental; Dental Enamel; Dental caries; Dentin; Dependence; Deposition; Devices; Dopa; Drug Formulations; Environment; Failure; Film; Friction; Generations; Glass; Glues; Human; Hydroxyapatites; Marines; Mass Spectrum Analysis; Measurement; Measures; Mechanics; Mica; Minerals; Modeling; Molecular; Motion; Mussels; Outcome; Oxidation-Reduction; Performance; Physics; Plant Resins; Polymers; Post-Translational Protein Processing; Property; Protein Biochemistry; Proteins; Stress; Surface; Techniques; Technology; Testing; Thick; Titania; Titanium; Tooth structure; Translating; United States National Institutes of Health; Variant; Water; base; bone; chemical binding; clinically relevant; dental resin; foot; glass ionomer; improved; oxidation; public health relevance; repaired; restorative dentistry; sensor

DESCRIPTION (provided by applicant): Polymer adhesion to wet mineral surfaces is typically limited by the lack of polymer- surface interactions strong enough to compete with water. Marine mussels overcome this limitation by using a suite of specific DOPA-containing proteins that chemically bind even to wet, atomically smooth surfaces. Protein biochemistry and surface physics are combined in this proposal to investigate the adhesive strategies of mussels on surfaces of hydroxyapatite - the mineral of tooth and bone. In the first aim, mass spectrometry and molecular surface sensors will be used to interrogate the proteins, pH, redox, and water fastness of adhesive secretions deposited onto hydroxyapatite. In aim 2, hydroxyapatite-specific proteins will be tested for adhesion in the surface forces apparatus using the pH and redox conditions used in mussel adhesion. In the third aim, a 3-dimensional surface forces apparatus will be introduced to measure the effect of multidirectional motion on the dynamic adhesion of mussel-derived proteins to dentinal and enamel surfaces.

描述（由申请人提供）：聚合物对湿矿物表面的粘附通常受到缺乏足够强的聚合物表面相互作用而限制与水的竞争。海洋贻贝通过使用一套含有DOPA的特定蛋白质来克服这一局限性，这些蛋白质甚至可以在湿，原子光滑的表面上结合。该提案合并了蛋白质生物化学和表面物理学，以研究贻贝在羟基磷灰石表面上的粘合剂策略 - 牙齿和骨骼的矿物质。在第一个目标中，质谱和分子表面传感器将用于询问沉积在羟基磷灰石上的粘合剂分泌物的蛋白质，pH，氧化还原和水牢度。在AIM 2中，使用贻贝粘附中使用的pH和氧化还原条件，将测试羟基磷灰石特异性蛋白在表面力设备中的粘附。在第三个目标中，将引入三维表面力设备，以测量多向运动对贻贝衍生蛋白在牙本质和搪瓷表面的动态粘附的影响。