Transating Mussel Adhesion

平移贻贝粘附力

• 批准号: 8729438
• 负责人: JACOB N ISRAELACHVILI
• 金额: $ 41.29万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-04 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8729438
• 关键词: 3-Dimensional; Acids; Adhesions; Adhesives; Age; Binding; Collagen; Collagen Type I; Dental; Dental Enamel; Dental caries; Dentin; Dependence; Deposition; Devices; Dopa; Drug Formulations; Environment; Failure; Film; Friction; Generations; Glass; Glues; Human; Hydroxyapatites; Marines; Mass Spectrum Analysis; Measurement; Measures; Mechanics; Mica; Minerals; Modeling; Molecular; Motion; Mussels; Outcome; Oxidation-Reduction; Performance; Physics; Plant Resins; Polymers; Post-Translational Protein Processing; Property; Protein Biochemistry; Proteins; Stress; Surface; Techniques; Technology; Testing; Thick; Titania; Titanium; Tooth structure; Translating; United States National Institutes of Health; Variant; Water; base; bone; chemical binding; clinically relevant; dental resin; foot; glass ionomer; improved; oxidation; public health relevance; repaired; restorative dentistry; sensor

DESCRIPTION (provided by applicant): Polymer adhesion to wet mineral surfaces is typically limited by the lack of polymer- surface interactions strong enough to compete with water. Marine mussels overcome this limitation by using a suite of specific DOPA-containing proteins that chemically bind even to wet, atomically smooth surfaces. Protein biochemistry and surface physics are combined in this proposal to investigate the adhesive strategies of mussels on surfaces of hydroxyapatite - the mineral of tooth and bone. In the first aim, mass spectrometry and molecular surface sensors will be used to interrogate the proteins, pH, redox, and water fastness of adhesive secretions deposited onto hydroxyapatite. In aim 2, hydroxyapatite-specific proteins will be tested for adhesion in the surface forces apparatus using the pH and redox conditions used in mussel adhesion. In the third aim, a 3-dimensional surface forces apparatus will be introduced to measure the effect of multidirectional motion on the dynamic adhesion of mussel-derived proteins to dentinal and enamel surfaces.

描述（由申请人提供）：聚合物对湿矿物表面的粘附力通常由于缺乏足够强以与水竞争的聚合物-表面相互作用而受到限制。海洋贻贝通过使用一套特定的含有多巴的蛋白质克服了这一限制，这些蛋白质甚至可以化学结合到潮湿的、原子级光滑的表面。该提案将蛋白质生物化学和表面物理学相结合，研究贻贝在羟基磷灰石（牙齿和骨骼的矿物质）表面上的粘附策略。第一个目标是使用质谱和分子表面传感器来检测沉积在羟基磷灰石上的粘性分泌物的蛋白质、pH、氧化还原和耐水性。在目标 2 中，将使用贻贝粘附所用的 pH 和氧化还原条件，在表面​​力装置中测试羟基磷灰石特异性蛋白质的粘附力。第三个目标是引入三维表面力装置来测量多向运动对贻贝源性蛋白质与牙质和牙釉质表面动态粘附的影响。