Tissue-dynamics Imaging for Therapeutic Efficacy in Ovarian Cancer

组织动力学成像对卵巢癌治疗效果的影响

基本信息

批准号：
8656327
负责人：
Daniela E Matei
金额：
$ 32.18万
依托单位：
PURDUE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-05-01 至 2017-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8656327
关键词：
Accounting Ascites Asses Bedside Testings Biological Biological Assay Biological Markers Biological Sciences Cancer Biology Cancer Center Cancer cell line Cell Line Cell Proliferation Cell physiology Cells Cellular Spheroids Cessation of life Clinic Clinical Clinical Trials Complex Development Diagnosis Disease Drug resistance Epithelial ovarian cancer Fatal Outcome Fingerprint Functional Imaging Future Goals Heterogeneity Holography Human Hypoxia Image Imaging technology Implant In Vitro Lighting Malignant Female Reproductive System Neoplasm Malignant Neoplasms Malignant neoplasm of ovary Measurement Measures Metastatic to Modeling Monitor Motion Neoplasm Metastasis Operative Surgical Procedures Optics Ovarian Patients Peritoneal Pharmaceutical Preparations Physics Platinum Primary Neoplasm Process Property Recurrence Relative (related person)Resistance Science Solid Neoplasm Source Specimen Staging Stratification System Taxane Compound Technology Technology Transfer Testing Therapeutic Therapeutic Agents Tissues Translating Treatment Efficacy Treatment Protocols Tumor-Derived United States Woman Work Xenograft procedure base cancer care cancer cell cancer therapy chemotherapy clinical application clinically relevant design digital drug efficacy drug mechanism experience improved in vitro Model innovation intraperitoneal light scattering malignant ascites millimeter new technology novel ovarian neoplasm physical science public health relevance research study response response marker screening taxane tumor tumor microenvironment

项目摘要

DESCRIPTION (provided by applicant): This project will employ a non-traditional imaging technology called tissue-dynamics imaging (TDI) to measure the sensitivity of ovarian tumors to chemotherapy and biological treatments. Tissue-dynamics imaging is the first imaging technology to use intracellular motion as an internal vital-signs monitor of cancer tissue. TDI uses digital holography with low-coherence illumination to construct 3D holograms up to a millimeter inside tissue. Dynamic imaging detects diverse cellular motions as endogenous image contrast in a functional imaging approach. Three-dimensional solid tumor models, such as tumor exgrafts and multicellular tumor spheroids, are ideal in vitro models to study complex three-dimensional tumor microenvironments, tumor heterogeneity, and multicellular drug resistance. Tissue dynamics imaging provides the required depth capability, the sensitivity to cellular motions, and the signatures of different dynamical cellular functions to provide quantitative measurements of cellular functioning and proliferation. In the proposed work, tissue dynamics imaging will be used to generate the first drug-response phenotypic profiles in cancer biology applied to tumor exgrafts. The goal is to design a reproducible and sensitive predictive marker to therapy. Current approaches that measure chemo-resistance are cumbersome and not predictive of biological and clinical response. By bridging between the fields of cancer biology and coherent optical physics, we propose that by exploiting the intracellular dynamical properties of ovarian tumors or metastatic implants ex-vivo, this new technology can be adapted to overcome a problem of high clinical relevance for women with ovarian cancer. A commercial partner, Animated Dynamics LLC, will receive technology transfer and construct the first clinic-based TDI system.

描述（由申请人提供）：该项目将采用一种称为组织动力学成像（TDI）的非传统成像技术，以测量卵巢肿瘤对化学疗法和生物学治疗的敏感性。组织动力学成像是第一个将细胞内运动用作癌症组织内生命符号监测仪的第一个成像技术。 TDI使用具有低碳化照明的数字全息图来构建3D全息图，直至毫米内组织。动态成像在功能成像方法中将各种细胞运动视为内源性图像对比度。三维实体瘤模型，例如肿瘤外植物和多细胞肿瘤球体，是研究复杂的三维肿瘤微环境，肿瘤异质性和多细胞耐药性的理想体外模型。组织动力学成像提供了所需的深度能力，对细胞运动的敏感性以及不同动态细胞功能的特征，以提供细胞功能和增殖的定量测量。在拟议的工作中，将使用组织动力学成像来生成应用于肿瘤外移植物的癌症生物学中的第一个药物反应表型。目的是为治疗设计可再现和敏感的预测标记。当前测量化学抗性的方法是繁琐的，无法预测生物学和临床反应。通过在癌症生物学和相干的光学物理学领域之间进行桥接，我们建议通过利用卵巢肿瘤或转移性植入物的细胞内动力学特性，可以将这项新技术改编以克服卵巢癌女性的高临床相关性。商业合作伙伴Animated Dynamics LLC将接收技术转移并构建第一个基于诊所的TDI系统。