Genomics

基因组学

基本信息

批准号：
8720766
负责人：
AARON W BELL
金额：
$ 11.22万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8720766
关键词：
Adenoviruses Adult Bioinformatics Biology Childhood Cirrhosis Clinical Data Dependovirus Disease Effectiveness Freezing Gene Expression Gene Expression Microarray Analysis Gene Expression Profile Gene Expression Profiling Generations Genes Genomics Hepatic Human Human Resources Hybridization Array Lead Literature Liver Liver Fibrosis Liver diseases Metabolic MicroRNAs Modality Mus Oligonucleotides Pathology Preparation Primary carcinoma of the liver cells Process Protein C Inhibitor Quality Control RNA RNA Interference Rattus Recombinant adeno-associated virus (rAAV)Research Research Personnel Sampling Small Interfering RNA Specificity Statistical Data Interpretation Subfamily lentivirinae System Tissues Training Transgenes Viral Vector Virus adeno-associated viral vector base cDNA Arrays design experience gene therapy knock-down overexpression professor programs research study therapeutic target transgene expression vector virus core

项目摘要

CORE C : Genomics Core (Two components: Affymetrix and Virus ) 1: Affymetrix microarray analysis of gene expression: Several components of this project will require analysis of gene expression in whole or micro-dissected liver tissue, from mouse and human samples. These studies will be performed in the genomics core for research of the department of Pathology. This core utilizes state of the art Affymetrix oligo-based cDNA microarrays (Mouse, human, C.Elegans, etc) for quantitative gene expression analysis. This facility is directed by Dr. Jian-Hua Luo, associate professor, department of pathology. Samples are provided as frozen tissue, on which, the facility performs RNA extraction and all other processing steps and provides the final data/results. This facility routinely processes human, mouse and rat samples and provides bio-informatic supportfor statistical analysis and data interpretation. 2: Virus core: Several components of this project will require genetic interventions readily supplied by Adeno-Associated Virus(AAV), recombinant adenovirus or Lentivirus. The virus core will collaborate with all investigators in this project for the design and preparation of suitable AAV or lentiviral based vectors to deliver transgenes and/or silencing RNA for overexpression or gene knock-down studies. This core will be directed by Dr. Aaron Bell, assistant professor, department of Pathology, who is highly experienced in AAV vector design. These approaches are well established in hepatic biology literature, and is already being used by Drs. Bell and Michalopoulos in collaborative research unrelated to this project which has validated its specificity and effectiveness. The AAV vectors allow for expression of transgenes up to 5KB and easily accommodates siRNA expression cassettes for gene knock-down studies.

Core C：基因组学核心（两个组成部分：Affymetrix和病毒） 1：基因表达的Affymetrix微阵列分析：该项目的几个组成部分需要从小鼠和人类样品中分析全部或微删除的肝组织中基因表达。这些研究将以病理学研究的基因组核心进行。这个核心利用Art Affymetrix Oligo的状态cDNA微阵列（小鼠，人，C.Elegans等）定量基因表达分析。该设施由副教授Jian-hua Luo博士指导。病理学系。样品作为冷冻组织提供，设施在其上执行RNA 提取和所有其他处理步骤，并提供最终的数据/结果。该设施通常会处理人，小鼠和大鼠样品，并提供生物形式支持，以提供统计分析和数据解释。 2：病毒核心：该项目的几个组成部分将需要易于提供的遗传干预措施病毒（AAV），重组腺病毒或慢病毒。病毒核心将与所有研究人员合作设计和准备合适的AAV或基于慢病毒的向量以交付转基因和/或沉默的RNA进行过表达或基因敲除研究。这个核心将由亚伦·贝尔博士执导病理学系助理教授，他在AAV矢量设计方面经验丰富。这些方法在肝生物学文献中已经建立了很好的确立，并且已经被Drs使用了。贝尔和 Michalopoulos在合作研究中与该项目无关，该项目验证了其特异性和效力。 AAV矢量允许表达高达5KB的转基因，并轻松适应 siRNA表达盒用于基因敲低研究。