Multiwalled-Carbon Nanotube Properties and Macrophage Proinflammatory Responses

多壁碳纳米管特性和巨噬细胞促炎症反应

基本信息

批准号：
8625977
负责人：
Paul Pantano
金额：
$ 45.9万
依托单位：
UNIVERSITY OF TEXAS DALLAS
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-03-01 至 2018-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8625977
关键词：
Address Adverse effects Animal Model Animals Asbestos Biological Bovine Serum Albumin Caliber Carbon Nanotubes Cell Extracts Cell membrane Cell model Cells Chemistry Collaborations Conflict (Psychology)Cytoplasm Cytosol Environment Event Experimental Designs Foundations Health Human Hydrogen Bonding Image Inflammation Interleukin-1 Lasers Lead Length Literature Lung Measures Membrane Membrane Lipids Mesothelioma Methods Microscopy Modeling Nanotubes Outcome Phagocytes Phagolysosome Pluronics Polyacrylamide Gel Electrophoresis Powder dose form Preparation Production Property Proteins Protocols documentation Publishing Pulmonary Fibrosis Reporting Research Resolution Review Literature Route Scanning Sodium Dodecyl Sulfate Surface Surveys System TNF gene Time Toxic effect Vendor Work base cell injury cellular imaging cytokine cytotoxicity in vivo macrophage multi walled carbon nanotube novel strategies public health relevance rapid technique research study response three-dimensional modeling uptake

项目摘要

The production and use of multi-walled carbon nanotubes (MWNTs) are rapidly increasing world-wide, despite the possible adverse effects they may have on human health. Of particular concern are reports that MWNTs can cause pulmonary fibrosis in lab animals that may lead to mesothelioma, similar to asbestos. The literature is conflicted regarding what physical features of MWNTs cause phagocytic cells to release proinflammatory cytokines that precede pulmonary fibrosis. It has also been suggested that phagolysosome membrane damage caused by MWNTs is a key event leading to cytokine release by phagocytic cells, but what MWNT properties cause damage is also not clear. The broad objectives of the research proposed here are to better understand whether MWNT length and extent of dispersion are factors leading to cytokine release by phagocytic cells and to explore a relatively new approach, laser scanning confocal Raman microscopy, to assess whether MWNTs damage the phagolysosomal membrane en route to eliciting cytokine release. There are two specific aims to achieve the objectives of the proposal: Specific aim 1: To quantify the amount of MWNTs taken up by two types of phagocytic cells and to correlate the uptake with MWNT length, dispersant, agglomeration, toxicity, and the release of proinflammatory cytokines. Two different MWNT types will be studied, long and short. For each type, two dispersions will be prepared with the dispersants bovine serum albumin or Pluronic(R) F-108, highly dispersed and agglomerated. The experimental design will use a new method to measure the actual amount of the MWNTs that accumulate inside cultured phagocytic cells, followed by measuring the release of two cytokines, TNF-¿ and IL-1¿. The results of this aim will, for the first time, correlate the actual amount of MWNTs inside phagocytic cells with MWNT length, type of dispersant, agglomeration, toxicity, and the release of proinflammatory cytokines. Specific aim 2: To determine the subcellular distribution of MWNTs and potential phagolysosome damage by laser scanning confocal Raman microscopy (LSCRM). LSCRM, a relatively new approach to cell imaging, can directly locate MWNTs inside cells by their unique Raman signature and be used to reconstruct 3D models from confocal sections showing the subcellular distribution of MWNTs. LSCRM can also image membranes in cells based on the C-H bond Raman scattering of membrane lipids. With this combination of capabilities it may be possible to determine whether MWNTs taken up by cells damage the phagolysosome membrane and redistribute throughout the cytosol. Results from this aim should help address whether MWNT length and state of dispersion are factors in damaging the phagolysosome membrane.

多壁碳纳米管（MWNT）的生产和使用正在全球范围内迅速增加它们可能对人类健康产生的不利影响。特别关注的是MWNT的报道可能导致间皮瘤的实验动物引起肺纤维化，类似于石棉。文学在MWNT的哪些物理特征引起吞噬细胞释放促炎性的情况下，存在冲突肺纤维化之前的细胞因子。还建议吞噬膜体膜由MWNT造成的损害是导致吞噬细胞释放细胞因子的关键事件，但是什么mwnt 属性造成损害也不清楚。这里提出的研究的广泛对象是更好的了解MWNT的长度和分散程度是否是导致细胞因子释放的因素吞噬细胞并探索一种相对新方法，激光扫描共焦拉曼显微镜，评估MWNT是否会损害吞噬体膜，以引发细胞因子释放。那里是实现该提案目标的两个具体目标：特定目的1：量化两种类型的吞噬细胞和至将吸收与MWNT长度，分散剂，团聚，毒性和释放相关联促炎细胞因子。长而短的两种不同的MWNT类型将是研究的。对于每种类型，两个分散剂将与分散剂牛血清白蛋白或Pluronic（R）F-108一起制备，高度分散并结合。实验设计将使用一种新方法来测量在培养的吞噬细胞内积累的MWNT，然后测量两种细胞因子的释放， tnf-和IL-1。这个目标的结果将首次将实际的MWNT量相关联具有MWNT长度的吞噬细胞，分散剂类型，团聚，毒性和释放促炎细胞因子。特定目的2：确定MWNT的亚细胞分布和潜在的吞噬体激光扫描共聚焦拉曼显微镜（LSCRM）受到损害。 LSCRM，一种相对的新方法细胞成像，可以通过其独特的拉曼签名直接定位细胞内部的MWNT，并用于从共聚焦切片中重建3D模型，显示了MWNT的亚细胞分布。 LSCRM可以还基于膜脂质的C-H键拉曼散射在细胞中的图像膜。与此功能的结合可能可以确定细胞占用的MWNT是否损坏整个细胞质中的吞噬物质膜和重新分布。这个目标的结果应有助于解决 MWNT长度和分散状态是否是损害吞噬膜膜的因素。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The Importance of Evaluating the Lot-to-Lot Batch Consistency of Commercial Multi-Walled Carbon Nanotube Products.

DOI：
10.3390/nano10101930
发表时间：
2020-09-27
期刊：
Nanomaterials (Basel, Switzerland)
影响因子：
0
作者：
Huynh MT;Veyan JF;Pham H;Rahman R;Yousuf S;Brown A;Lin J;Balkus KJ Jr;Diwakara SD;Smaldone RA;LeGrand B;Mikoryak C;Draper R;Pantano P
通讯作者：
Pantano P

Use of Raman spectroscopy to identify carbon nanotube contamination at an analytical balance workstation.