Golgi Studies of Schizophrenia

精神分裂症的高尔基体研究

基本信息

批准号：
8448242
负责人：
ANDREW J DWORK
金额：
$ 48.63万
依托单位：
NEW YORK STATE PSYCHIATRIC INSTITUTE DBA RESEARCH FOUNDATION FOR MENTAL HYGIENE, INC
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-08-01 至 2016-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8448242
关键词：
Administrative Supplement Affect Age Amygdaloid structure Animal Model Apical Atrophic Autopsy Brain Brain region Cells Clinical Cognitive deficits Collection Cytoskeletal Proteins Data Dendrites Dendritic Spines Diffusion weighted imaging Dorsal Forensic Medicine Fresh Tissue Functional disorder Funding Glutamates Golgi Apparatus Golgi method Grant Hippocampal Formation Hippocampus (Brain)Horns Human Institutes Institution Laboratories Lead Left MFGE8 gene Macedonia Macedonia (Republic)Medial Medial Dorsal Nucleus Mental disorders Methods Microtubule-Associated Protein 2 Midbrain structure Modification Morphology Neurobiology Neurons Pattern Population Prefrontal Cortex Procedures Process Pyramidal Cells Random Allocation Reporting Sampling Schizophrenia Site Slide Specimen Staining method Stains Stress Structure Techniques Thalamic structure Tissue Banks Tissues Toxicology United States National Institutes of Health Vertebral column abstracting brain tissue cholinergic density dentate gyrus hippocampal pyramidal neuron human tissue immunoreactivity improved in vivo lateral ventricle neurogenesis neuronal cell body neuropathology prospective psychologic sex success tissue fixing white matter

项目摘要

Abstract Golgi staining reveals the anatomical structure of dendrites. While Golgi methods have yielded some of the most robust histological findings in schizophrenia, studies that use these techniques are uncommon, probably because the techniques have been difficult to apply and notoriously capricious. We have developed a new modification of the Golgi-Cox method that has yielded unprecedented reliability, with consistent staining in over 100 consecutive blocks from over 70 consecutive autopsy cases. There is a uniform distribution of stained neurons that allows systematic random sampling of neurons for analysis. We propose to use this material to study dendritic arborization and spine density in 20 schizophrenia subjects matched by age and sex to 40 subjects without psychiatric disease, all evaluated clinically by psychological autopsy. We will analyze dendritic arborization and spine density in dorsal (BA9) and ventral (BA45) prefrontal cortex, and in the hippocampal formation (subiculum, CA3, CA1, and dentate gyrus). Since every section cut from a block is stained well, we propose to share the slides with other laboratories that wish to perform additional analyses. This application also includes continuation of our ongoing collection of brain specimens and psychological autopsies in the Republic of Macedonia. This collection provides the fresh tissue for our Golgi studies and for many other studies of the neurobiology of psychiatric illnesses, both in our department and at other institutions. The specimens and data are stored in our department and subjected to rigorous clinical, neuropathological, and toxicological review.

抽象的高尔基体染色揭示了树突的解剖结构。而高尔基方法已经在精神分裂症方面取得了一些最有力的组织学发现，研究表明使用这些技术并不常见，可能是因为这些技术已经被难以应用且反复无常。我们开发了一种新的修改高尔基-考克斯方法具有前所未有的可靠性和一致的染色效果来自 70 多个连续尸检病例的 100 多个连续区块。有一个染色神经元的均匀分布，允许系统随机采样神经元进行分析。我们建议使用这种材料来研究树枝状树枝化和 20 名精神分裂症受试者的脊柱密度（按年龄和性别）与 40 名受试者相匹配无精神疾病，均通过心理尸检进行临床评估。我们将分析背侧 (BA9) 和腹侧 (BA45) 的树突分枝和棘密度前额皮质和海马结构（下托、CA3、CA1 和齿状回）回）。由于从块上切下的每个部分都染色良好，因此我们建议分享与希望进行额外分析的其他实验室进行幻灯片。该应用程序还包括我们正在进行的大脑收集的延续马其顿共和国的标本和心理尸检。这个合集为我们的高尔基体研究和许多其他研究提供新鲜组织我们部门和其他机构的精神疾病神经生物学。标本和数据均保存在我们部门并经过严格的临床、神经病理学和毒理学审查。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The contribution of HIV infection to intracranial arterial remodeling: a pilot study.

HIV 感染对颅内动脉重塑的贡献：一项试点研究。

DOI：
发表时间：
2013-06
期刊：
影响因子：
0
作者：
Gutierrez, Jose;Elkind, Mitchell S V;Petito, Carol;Chung, David Y;Dwork, Andrew J;Marshall, Randolph S
通讯作者：
Marshall, Randolph S

Hippocampal granule neuron number and dentate gyrus volume in antidepressant-treated and untreated major depression.

抗抑郁药治疗和未治疗的重度抑郁症中海马颗粒神经元数量和齿状回体积。

DOI：
发表时间：
2013-05
期刊：
影响因子：
0
作者：
Boldrini, Maura;Santiago, Adrienne N;Hen, René;Dwork, Andrew J;Rosoklija, Gorazd B;Tamir, Hadassah;Arango, Victoria;John Mann, J
通讯作者：
John Mann, J

Hippocampal angiogenesis and progenitor cell proliferation are increased with antidepressant use in major depression.

在重度抑郁症中使用抗抑郁药物会增加海马血管生成和祖细胞增殖。

DOI：
发表时间：
2012-10-01
期刊：
影响因子：
10.6
作者：
Boldrini, Maura;Hen, René;Underwood, Mark D;Rosoklija, Gorazd B;Dwork, Andrew J;Mann, J John;Arango, Victoria
通讯作者：
Arango, Victoria

Psychiatric brain collection in Macedonia: general lessons for scientific collaboration among countries of differing wealth.

马其顿的精神病学大脑收集：不同财富国家之间科学合作的一般教训。