Core C - FACS and Epigenetics Technology Core

核心C - FACS和表观遗传学技术核心

• 批准号: 8695330
• 负责人: YI ZHOU
• 金额: $ 32.57万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8695330
• 关键词: Biological Models; Blood; Blood Cells; Cell Count; Cell Separation; Cell surface; Cells; Chromatin Structure; Development; Disease; Disease model; Epigenetic Process; Fluorescence; Gene Expression; Gene Expression Profiling; Hematopoiesis; Hematopoietic; Mammalian Cell; Molecular; Population; Services; Sorting - Cell Movement; Technology; Transgenic Organisms; Zebrafish; chromatin immunoprecipitation; human disease; innovation; member; next generation; next generation sequencing; novel therapeutic intervention; technology development

and zebrafish model systems developed in the Center. These technologies, fluorescence activated ceil sorting (FACS) and epigenetic analysis on small numbers of cells, will provide an unprecedented view of hematopoiesis. The recent development of technologies such as chromatin immunoprecipitation and next generation sequencing (ChlP-seq) promises to dramatically enhance our understanding of gene expression in hematopoietic cells. CORE C will provide Center members with ready access to the latest, most advanced cell sorting capabilities, and will further develop, apply, and make widely available the most innovative approaches to epigenetic analyses on limiting numbers of primary cells. The efforts of CORE C will significantly lower the barrier to application of these technologies. Significance: A detailed understanding of blood cell development during normal hematopoiesis and various disease states has direct relevance to human disease and will aid in the development of new therapeutic approaches.

和中心开发的斑马鱼模型系统。这些技术，荧光激活细胞分选 （FACS）和对少量细胞的表观遗传分析，将提供前所未有的观点 造血作用。染色质免疫沉淀等技术的最新发展 世代测序 (ChlP-seq) 有望显着增强我们对基因表达的理解 造血细胞。 CORE C 将为中心成员提供最新、最先进的单元 分拣能力，并将进一步开发、应用和广泛使用最具创新性的方法 对有限数量的原代细胞进行表观遗传学分析。 CORE C 的努力将显着降低 应用这些技术的障碍。意义：详细了解正常造血过程中血细胞的发育和 各种疾病状态与人类疾病直接相关，将有助于开发新的疾病 治疗方法。