Role of CDC14B in mouse oocyte maturation

CDC14B 在小鼠卵母细胞成熟中的作用

基本信息

批准号：
8599326
负责人：
Karen A Schindler
金额：
$ 23.25万
依托单位：
RUTGERS, THE STATE UNIV OF N.J.
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-01 至 2015-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8599326
关键词：
Aneuploidy BUB1 gene Back Cell Cycle Cells Centromere Chromatin Chromosome Segregation Chromosomes Commit Complex Cytokinesis DNA biosynthesis Defect Development Diploidy Disease Down Syndrome Embryo Embryonic Development Ensure Environment Faculty Family Female Financial compensation Funding Genetic Germ Cells Goals Haploidy Haspin Histones Human In Vitro Institution Kinetochores Knockout Mice Lead Link Mammals Meiosis Meiotic M Phase Metaphase Microtubules Mitosis Mitotic Mitotic Cell Cycle Molecular Mus Mutant Strains Mice New Jersey Oocytes Pennsylvania Phase Phosphoric Monoester Hydrolases Phosphotransferases Ploidies Positioning Attribute Postdoctoral Fellow Process Prophase Protein Kinase Protein phosphatase Proteins Reagent Recruitment Activity Regulation Reproduction Reproductive Biology Research Research Personnel Resources Role S Phase Signal Transduction Signaling Molecule Somatic Cell Spontaneous abortion Testing Time Tissues Universities Work analog arm aurora kinase aurora-A kinase base career egg mouse genome mouse model oocyte maturation precursor cell programs recombinase segregation sperm cell stillbirth unpublished works

项目摘要

Haploid gametes, that are essential for sexual reproduction, are generated fronn diploid precursor cells via meiosis. In females, oocytes undergo 2 major meiosis-specific, cell-cycle transitions wtiose regulation are poorly understood. Thie first transition is meiotic resumption where oocytes exit a prolonged prophase arrest and enter the first meiotic M phase (Ml), and the second is the transition between Ml and Mil where homologous chromosomes separate and no round of DNA synthesis occurs. This project began while the PI (Karen Schindler) was a postdoctoral fellow at the University of Pennsylvania and has facilitated transition to an independent research career. These studies investigating the molecular mechanisms that lead to aneuploidy in female eggs will form the basis of an independent research program in the Department of Genetics at Rutgers, The State University of New Jersey where the PI will assume a tenure-track faculty position in January 2012. The environment at Rutgers for research in reproductive biology and aneuploidy is outstanding, all resources required to complete the studies are available, and the institution is committed to promoting development of new investigators. Thus, the PI will be well-positioned to compete for additional funding and to have a successful independent research career.

单倍体配子是有性生殖所必需的，是由二倍体前体细胞通过减数分裂。在女性中，卵母细胞经历 2 个主要的减数分裂特异性细胞周期转变，其中的调节是不太了解。第一个转变是减数分裂恢复，其中卵母细胞退出长期的前期停滞并进入第一个减数分裂M期（Ml），第二个是Ml和Mil之间的过渡，其中同源染色体分离并且不发生一轮DNA合成。该项目开始时，PI（Karen Schindler）是伦敦大学的博士后研究员。宾夕法尼亚州并促进了向独立研究职业的过渡。这些研究调查导致雌性卵子非整倍性的分子机制将构成独立的基础新泽西州立大学罗格斯大学遗传学系的研究项目 PI 将于 2012 年 1 月担任终身教职。罗格斯大学的研究环境生殖生物学和非整倍性非常出色，完成研究所需的所有资源都是可用，并且该机构致力于促进新研究人员的发展。因此，PI 将处于有利地位，可以竞争额外的资金并拥有成功的独立研究生涯。