Nerve conduction block in cold-responsive sensory neurons

冷反应性感觉神经元的神经传导阻滞

基本信息

批准号：
8773913
负责人：
David D McKemy
金额：
$ 35.97万
依托单位：
UNIVERSITY OF SOUTHERN CALIFORNIA
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-05-15 至 2019-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8773913
关键词：
Affect Afferent Neurons Agonist Anesthetics Animals Behavior Behavioral Biological Assay Cations Cells Charge Chronic Data Esthesia Fluorescent Dyes Genetic Heating Hypersensitivity In Vitro Inflammatory Injection of therapeutic agent Injury Ion Channel Lidocaine Local Anesthetics Mammals Mechanics Mediating Menthol Modality Modeling Motor Mus Nerve Nerve Block Neural Conduction Neurons Neuropathy Nociception Nociceptors Pain Pathology Permeability Phenotype Population Reporting Residual state Role Signal Pathway Signal Transduction Sodium Specificity TRPA1 Channel TRPV1 gene Time Whole-Cell Recordings base cell type channel blockers chronic pain efficacy testing inflammatory neuropathic pain mouse model novel novel strategies pain behavior perineural prevent public health relevance receptor research study response sensor somatosensory uptake voltage

项目摘要

DESCRIPTION (provided by applicant): The menthol receptor TRPM8 is considered the principal cold sensor in mammalian sensory neurons as animals lacking TRPM8 function are deficient in cold and cold pain behaviors. However some residual cold sensitivity remains, indicating the possible presence of TRPM8-independent cold transduction mechanisms. Other cell types, such as those expressing TRPV1 and TRPA1 channels, are critical for somatosensory signaling, and have been implicated in certain aspects of cold sensation. Genetic approaches to determine the role of these channels and cell-types are complicated by the fact that the resulting phenotypes are investigated either many days after manipulation, or in developmentally disparate backgrounds. An elegant approach has recently been devised that targets cell impermeant sodium (Na+) channel blockers to only primary sensory neurons mediating pain (nociceptors). Specifically, when stimulated with agonists for nociceptor-specific TRPV1 and TRPA1 channels, large molecules such as the charged lidocaine derivative QX-314 permeate through these channels, thereby selectively blocking nerve conduction in just these neuronal populations. Previous reports failed to find large molecule entry through TRPM8 channels, suggesting that TRPM8 neurons cannot be targeted in this manner. Our underlying hypothesis, supported by our novel preliminary data, is that large molecules permeate cells through TRPM8 when the channel is activated by potent agonists, and we propose to determine if cold and cold pain can be ameliorated by selectively blocking nerve conduction of these and other neuronal populations. Aim 1 will determine the mechanisms whereby TRPM8 channels permeate large cations in vitro and be used as a means to target Na+-channel blockers to TRPM8 neurons. Aim 2 will determine if targeting Na+-channel blockers in TRPM8 neurons alters cold sensation in mice, and determine if other primary sensory neurons also contribute to cold. Aim 3 will extend these behavioral analyses to determine if chronic cold pain induced by injury can be ameliorated by targeting Na+-channel blockers to TRPM8, TRPV1, or TRPA1 neurons. With these studies we will determine if cold and cold pain can be specifically inhibited by the selective entry of large, cell impermeant anesthetics, as well as use this novel approach to further define the cellular basis for cold and cold pain, including identifying TRPM8-independent neuronal populations that contribute to this somatosensory modality.

描述（由申请人提供）：薄荷醇受体TRPM8被认为是哺乳动物感觉神经元中的主要冷传感器，因为缺乏TRPM8功能的动物缺乏冷和冷疼痛行为。然而，仍然存在一些残留的冷敏感性，表明可能存在与TRPM8无关的冷可传输机制。其他细胞类型，例如表达TRPV1和TRPA1通道的细胞类型，对体感信号传导至关重要，并且与冷感觉的某些方面有关。确定这些通道和细胞类型的作用的遗传方法使所产生的表型在操纵后很多天或在发育不同的背景中进行了复杂。最近，设计了一种优雅的方法，该方法将细胞无渗透钠（Na+）通道阻滞剂靶向仅介导疼痛（伤害感受器）的主要感觉神经元。具体而言，当用激动剂刺激伤害感受器特异性TRPV1和TRPA1通道时，大分子（例如带电的利多卡因衍生物QX-314）渗透到这些通道中，从而选择性地阻止这些神经元种群中的神经传导。先前的报告未能通过TRPM8通道找到大分子进入，这表明无法以这种方式靶向TRPM8神经元。我们的基本假设得到了新的初步数据的支持，即当通道被有效的激动剂激活时，大分子通过TRPM8渗透到细胞，我们建议通过选择性地阻断这些和其他神经元种群的神经传导，以确定是否可以改善冷和冷疼痛。 AIM 1将确定TRPM8通道在体外渗透大阳离子的机制，并用作将Na+通道阻滞剂靶向TRPM8神经元的手段。 AIM 2将确定TRPM8神经元中Na+通道阻滞剂的靶向是否会改变小鼠的冷感，并确定其他原发性感觉神经元是否也有助于感冒。 AIM 3将扩展这些行为分析，以确定通过将Na+通道阻滞剂靶向TRPM8，TRPV1或TRPA1神经元，可以改善损伤引起的慢性冷痛。通过这些研究，我们将确定是否可以通过选择性地进入大型细胞不足麻醉药来特别抑制冷疼痛，并使用这种新颖的方法进一步定义了冷和冷疼痛的细胞基础，包括识别对这种体征模式有助于TRPM8独立的神经元群体。