Synaptic circuitry mechanism for auditory cortical processing

听觉皮层处理的突触电路机制

基本信息

批准号：
8762331
负责人：
Li I Zhang
金额：
$ 41.14万
依托单位：
UNIVERSITY OF SOUTHERN CALIFORNIA
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-08-01 至 2019-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8762331
关键词：
Animals Area Auditory Auditory area Behavioral Cells Cognitive Complex Development Electrophysiology (science)Equilibrium Exhibits Feedback Frequencies Funding Head Image Individual Intracellular Membranes Lateral Lead Mediating Membrane Potentials Mental disorders Methods Molecular Genetics Mus Neurons Output Parvalbumins Pathology Pathway interactions Pattern Physiology Play Preparation Process Property Pyramidal Cells Recruitment Activity Role Sensory Sensory Process Shapes Signal Transduction Slice Somatostatin Stimulus Structure Synapses Techniques Testing Thalamic structure Whole-Cell Recordings awake base behavior influence cell type hippocampal pyramidal neuron in vivo inhibitory neuron innovation insight nerve supply nervous system disorder neural circuit optogenetics patch clamp public health relevance response sound tool two-photon voltage clamp

项目摘要

DESCRIPTION (provided by applicant): Inhibitory synaptic circuits play important roles in shaping cortical processing. Our understanding of the functional engagement of inhibitory circuits composed of different inhibitory cell types however remains poor. The recent development of molecular and genetic tools in the mouse, in combination with the innovative techniques of in vivo electrophysiology, has now made it possible to systematically dissect synaptic circuitry underlying specific cortical functions. In this project, we will integrate multile approaches to investigate the synaptic, in particular inhibitory circuitry mechanisms underlying auditory processing in the mouse primary auditory cortex (A1). In the first part, we will apply in vivo cell-attached and whole-cell recordings to investigate synaptic mechanisms for specific laminar processing in A1, a direct extension of the previously funded project. Second, we will combine in vivo two-photon imaging and patch-clamp recordings and utilize optogenetic methods to dissect functional roles of different types of inhibitory neuron. Finally, with high-quality whole-cell recordings in awake behaving mice, we will investigate cortical synaptic circuitry mechanisms for auditory processing functions in awake cortex, and their modulation by different behavioral states.

描述（由申请人提供）：抑制性突触电路在塑造皮质加工方面起着重要作用。但是，我们对由不同抑制性细胞类型组成的抑制回路的功能参与仍然很差。现在，小鼠中分子和遗传工具的最新发展以及体内电生理学的创新技术结合了现在使系统解剖特定皮质功能的突触电路的可能性。在该项目中，我们将整合多种方法来研究突触，特别是在小鼠主听觉皮层（A1）中进行听觉处理的抑制性电路机制（A1）。在第一部分中，我们将应用体内细胞连接和全细胞记录来研究A1中特定层流处理的突触机制，这是先前资助的项目的直接扩展。其次，我们将结合体内两光子成像和斑块钳记录，并利用光遗传学方法来剖析不同类型的抑制性神经元的功能作用。最后，在醒着的行为小鼠中，我们将研究清洁皮质中听觉处理功能的皮质突触电路机制，并通过不同的行为状态调节它们。