QUANTITATIVE ANALYSIS OF AGE SPECIFIC VARIATION IN THE ABUNDANCE OF HUMAN FEMALE

人类女性丰度的年龄特异性变化的定量分析

• 批准号: 8365791
• 负责人: JAMES E MELVIN
• 金额: $ 0.74万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365791
• 关键词: Age; Biological Markers; Biology; Categories; Charge; Chromatography; Disease; Ductal; Female; Foundations; Funding; Fungal Genome; Future; Grant; Human; Immune response; Lactoferrin; Link; Monitor; Muramidase; National Center for Research Resources; Parotid Gland; Principal Investigator; Proteins; Proteomics; Reporting; Research; Research Infrastructure; Resources; Saliva; Salivary Proteins; Source; Technology; Testing; United States National Institutes of Health; Variant; Western Blotting; Woman; age group; age related; cost; defense response; histatin 1; human female; human old age (65+); normal aging; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Human saliva is a protein-rich, easily accessible source of potential local and systemic biomarkers to monitor changes that occur under pathological conditions; however, little is known about the changes in abundance associated with normal aging. In this study, we performed a comprehensive proteomic profiling of pooled saliva collected from the parotid glands of healthy female subjects, divided into two age groups 1 and 2 (20-30 and 55-65 years old, respectively). Hydrophobic charge interaction chromatography was used to separate high- from low-abundance proteins prior to characterization of the parotid saliva using multidimensional protein identification technology (MudPIT). Collectively, 532 proteins were identified in the two age groups. Of these proteins, 266 were identified exclusively in one age group, while 266 proteins were common to both groups. The majority of the proteins identified in the two age groups belonged to the defense and immune response category. Of note, several defense related proteins (e.g., lysozyme, lactoferrin and histatin-1) were significantly more abundant in group 2 as determined by G-test. Selected representative mass spectrometric findings were validated by Western blot analysis. Our study reports the first quantitative analysis of differentially regulated proteins in ductal saliva collected from young and older female subjects. This study supports the use of high-throughput proteomics as a robust discovery tool. Such results provide a foundation for future studies to identify specific salivary proteins which may be linked to age-related diseases specific to women.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 人类唾液是一种富含蛋白质、易于获取的潜在局部和全身生物标志物来源，可用于监测病理条件下发生的变化；然而，人们对与正常衰老相关的丰度变化知之甚少。在这项研究中，我们对从健康女性受试者腮腺收集的唾液进行了全面的蛋白质组分析，将其分为两个年龄组 1 和 2（分别为 20-30 岁和 55-65 岁）。在使用多维蛋白质鉴定技术 (MudPIT) 表征腮腺唾液之前，使用疏水电荷相互作用色谱法分离高丰度蛋白质和低丰度蛋白质。两个年龄组总共鉴定出 532 种蛋白质。在这些蛋白质中，有 266 种仅在一个年龄组中被鉴定出，而 266 种蛋白质是两个组共有的。在两个年龄组中发现的大多数蛋白质属于防御和免疫反应类别。值得注意的是，根据 G 检验确定，第 2 组中的几种防御相关蛋白（例如溶菌酶、乳铁蛋白和组蛋白 1）明显更丰富。选定的代表性质谱结果通过蛋白质印迹分析进行验证。我们的研究报告了对从年轻和老年女性受试者收集的导管唾液中差异调节蛋白质的首次定量分析。这项研究支持使用高通量蛋白质组学作为强大的发现工具。这些结果为未来的研究奠定了基础，以确定可能与女性特有的年龄相关疾病有关的特定唾液蛋白。