Genetically Engineered Rodent Care

基因工程啮齿动物护理

• 批准号: 8718952
• 负责人: Gregg E. Homanics
• 金额: $ 31.17万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-05 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8718952
• 关键词: Adverse effects; Alcohol consumption; Alcohols; Alleles; Animals; Area; Behavioral; Breeding; Candidate Disease Gene; Caring; Consult; Consultations; Embryo; Ethanol; Gene Targeting; Genes; Genetic Engineering; Individual; Injection of therapeutic agent; Knock-out; Knockout Mice; Medical; Messenger RNA; Microinjections; Mus; Mutant Strains Mice; Organism; Production; Rat-1; Rattus; Research Personnel; Rodent; Role; Services; Societies; Staging; System; Technology; Testing; Transgenes; Transgenic Founder Animals; Transgenic Mice; Transgenic Organisms; Vendor; Zinc Fingers; alcohol research; blastocyst; design; design and construction; embryonic stem cell; gene function; model development; nuclease; response; vector

DESCRIPTION (provided by applicant): Genetically engineered rodents are essential for understanding gene function in the context of the whole animal. This is especially important for alcohol research as we ultimately seek to understand the role of individual candidate genes on endpoints that can only be studied at the organism level, e.g., alcohol drinking and alcohol-induced behavioral responses. To service the needs of the INIA-West for genetically engineered animals, this core will provide the following services to consortium investigators. Aim 1 services will provide consultation to investigators regarding model development and construct design. Aim 2 services all relate to gene targeted mouse production including production of targeting vectors, gene targeting in embryonic stem cells, production of chimerical mice, and breeding to establish mutant mouse lines. Transgenic mouse services are the focus of Aim 3. This aim includes transgene production, embryo microinjection, and identification of founder animals. Lastly, Aim 4 involves the application of recently developed zinc-finger nuclease technology to produce knockout rats. Zinc-finger nucleases will be purchased from a commercial vendor and this core will inject them into rat embryos and identify the gene targeted rats that result.

描述（由申请人提供）：基因工程啮齿动物对于在整个动物的背景下了解基因功能至关重要。这对于酒精研究尤其重要，因为我们最终寻求了解只能在生物体上进行研究的终点，例如饮酒和酒精引起的行为反应的作用。 为了满足Inia-West对基因工程动物的需求，该核心将为财团调查员提供以下服务。 AIM 1服务将向研究人员就模型开发和构造设计提供咨询。 AIM 2服务均与基因靶向小鼠的产生有关，包括靶向载体的产生，胚胎干细胞中的基因靶向，嵌合小鼠的产生以及建立突变小鼠系的繁殖。转基因小鼠服务是AIM 3的重点。该目标包括转基因生产，胚胎微注射和对创始人动物的识别。 最后，AIM 4涉及最近开发的锌指核酸酶技术来产生敲除大鼠。锌指核酸酶将从商业供应商那里购买，该核心将它们注入大鼠胚胎并识别导致的基因靶向大鼠。