HIGH RESOLUTION STRUCTURE OF BACTERIOPHAGE P22

噬菌体 P22 的高分辨率结构

• 批准号: 8362443
• 负责人: John Emil Johnson
• 金额: $ 0.64万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362443
• 关键词: Bacteriophage P22; Bacteriophages; Binding Proteins; Capsid; Cells; DNA; Double Stranded DNA Virus; Enzymes; Event; Funding; Grant; Herpesviridae; Infection; Microscopy; Molecular; Molecular Machines; National Center for Research Resources; Particle Size; Principal Investigator; Proteins; Pump; Research; Research Infrastructure; Resolution; Resources; Signal Transduction; Source; Structure; United States National Institutes of Health; cost; particle; pressure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Bacteriophages, herpesviruses and other large dsDNA viruses contain molecular machines that pump DNA into preassembled procapsids, triggering maturation with an associated cascade of particle size change, release of packaging enzymes, binding of proteins for cell association and activation of the particles for infection. These events are associated with capsid pressures exceeding, by 10-fold, that of bottled champagne, and are detected by a protein switch that transduces a signal from inside to outside of the particle.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 噬菌体、疱疹病毒和其他大型 dsDNA 病毒含有分子机器 将 DNA 泵入预组装的衣壳中，引发相关成熟 颗粒大小的级联变化、包装酶的释放、细胞蛋白质的结合 感染颗粒的关联和激活。 这些事件与 衣壳压力超过瓶装香槟的 10 倍，并通过 将信号从颗粒内部传递到外部的蛋白质开关。