BIOMASS TRANSFORMATION ? CATALYSIS OF ISPG

生物质转化？

• 批准号: 8365584
• 负责人: Pinghua Liu
• 金额: $ 0.46万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-08-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365584
• 关键词: Biology; Biomass; Catalysis; Digestion; Electron Transport; Engineering; Funding; Gel; Grant; Mass Spectrum Analysis; Medicine; National Center for Research Resources; Organism; Play; Principal Investigator; Production; Proteins; Reaction; Regulation; Research; Research Infrastructure; Resources; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; United States National Institutes of Health; catalyst; cost; improved; protein complex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. IspG is a protein of 41 kD, known to play a key role as a catalyst in the engineering production of host organisms for biofuel production. According to the research results of Liu and his coworkers, IspG is present as mutli-protein-complexes. Because IspG catalyses reductive deoxygenation reactions, their partner proteins are either involved in electron transfer or related to the regulation of IspG reduction potential. To have a better understanding of the mechanism and improve the IspG activity, we have undeertaken the identification of these proteins by mass spectrometry. In-gel digestion of each of these partners followed by analysis by MALDI-TOF MS and nanoLC-MS/MS on LTQ-Orbitrap (Thermo-Fisher) are being performed to identify these partners.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 IspG 是一种 41 kD 的蛋白质，已知在用于生物燃料生产的宿主生物工程生产中作为催化剂发挥着关键作用。根据Liu及其同事的研究结果，IspG以多蛋白复合物的形式存在。由于 IspG 催化还原性脱氧反应，因此它们的伴侣蛋白要么参与电子转移，要么与 IspG 还原电位的调节有关。为了更好地理解其机制并提高IspG活性，我们对这些蛋白进行了质谱鉴定。对这些伙伴中的每一个进行凝胶内消化，然后在 LTQ-Orbitrap (Thermo-Fisher) 上使用 MALDI-TOF MS 和 nanoLC-MS/MS 进行分析，以识别这些伙伴。