2D IR PHOTON ECHO OF AZIDO-PROBES FOR BIOMOLECULAR DYNAMICS

用于生物分子动力学的叠氮探针的 2D 红外光子回波

• 批准号: 8362582
• 负责人: Scott H Brewer
• 金额: $ 1.69万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362582
• 关键词: Azides; Biological; Drug Interactions; Environment; Enzyme Interaction; Funding; Goals; Grant; Laboratories; Measurement; Membrane; National Center for Research Resources; Nitriles; Nucleosides; Optics; Peptides; Photons; Principal Investigator; Process; Proteins; Research; Research Infrastructure; Resources; Signal Transduction; Site; Source; Spectrum Analysis; Stretching; United States National Institutes of Health; Water; Work; absorption; biological systems; cost; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Infrared markers, such as nitriles and azides, have become useful as vibrational probes of biomolecules. In particular, ultrafast measurements of the stretching mode of the nitrile group in biological systems have provided local site-specific vibrational dynamics. Several studies, including peptide membrane interactions and drugenzyme interactions, havetaken advantage of the relatively sharp spectral features of nitriles that occur in a spectrally transparent region of most biological molecules while having sensitivity to the local environment. However, the relatively small absorption cross section of the nitrile vibrational transition compromises its utility for nonlinear IR work and necessitates the use of higher concentrations than desirable for biological systems in both linear and nonlinear IR experiments; furthermore the absorption signal overlaps some water absorption bands. Thus, new vibrational probes are needed that are suitable to be used as biological probes. One promising candidate for nonlinear spectroscopy is the azido-, N3, group. The absorption cross section of azides is about one order of magnitude greater than nitriles allowing for much lower concentrations to be utilized. Also, the N3 transition, like CN, is sensitive to changes in the local environment. The aliphatic azides are stable under most conditions. The potential utility of the azido group as an infrared probe of local environment has been demonstrated in peptides and proteins. This utility has been further extended to nucleosides as infrared and NMR experiments. The azido-group has a relatively simple vibrational signature in the 2100 cm-1 region where one sharp transition is observed. The goal of this project is to investigate the potenital use of azides as environmental probes in 2D IR spectroscopy.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 红外标记物，例如腈和叠氮化物，已成为有用的生物分子振动探针。特别是，对生物系统中腈基拉伸模式的超快测量提供了局部特定位点的振动动力学。包括肽膜相互作用和药物酶相互作用在内的几项研究利用了腈类相对尖锐的光谱特征，这种特征出现在大多数生物分子的光谱透明区域，同时对局部环境具有敏感性。然而，腈振动跃迁的相对较小的吸收截面损害了其在非线性红外工作中的实用性，并且需要在线性和非线性红外实验中使用比生物系统所需的更高的浓度；此外，吸收信号与一些水吸收带重叠。 因此，需要适合用作生物探针的新振动探针。非线性光谱学的一个有希望的候选者是叠氮基，N3，基团。叠氮化物的吸收截面比腈大大约一个数量级，从而可以使用低得多的浓度。此外，N3 过渡与 CN 一样，对当地环境的变化很敏感。脂肪族叠氮化物在大多数条件下是稳定的。叠氮基作为局部环境红外探针的潜在用途已在肽和蛋白质中得到证实。这种实用性已进一步扩展到核苷，如红外和核磁共振实验。叠氮基团在 2100 cm-1 区域具有相对简单的振动特征，在该区域观察到急剧的跃迁。该项目的目标是研究叠氮化物作为二维红外光谱中环境探针的潜在用途。