INTERNATIONAL GENE TRAP CONSORTIUM (IGTC) RESOURCE

国际基因陷阱联盟 (IGTC) 资源

基本信息

批准号：
8363603
负责人：
THOMAS E FERRIN
金额：
$ 1.68万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-01 至 2012-06-30
项目状态：
已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. With the completion of the human and mouse genome sequences, there has been increased interest in developing tools for genome-wide mutagenesis in the mouse, with the goal of analyzing genome function in the context of the intact organism. Gene trapping is a high-throughput approach that can be used to introduce insertional mutations across the genome in mouse embryonic stem (ES) cells. Gene trap vectors simultaneously mutate and report the expression of the endogenous gene at the site of insertion and provide a DNA tag for the rapid identification of the disrupted gene. The generation of mutant mice from ES cell lines carrying gene trap insertions could be applied to large-scale functional analysis of mammalian genes. The International Trap Consortium (IGTC) consists of laboratories around the world working together to generate a public library of mutated murine ES cell lines. Such cell lines can be obtained on a non-collaborative basis by scientists interested in generating reporter-tagged, loss-of-function mutations in mice. In addition to loss of function, newer gene trap vectors offer a variety of post-insertional modification strategies to allow for the generation of other experimental alleles. The cooperative goal of the IGTC is to generate an international resource representing all or most genes in the mouse genome, and to provide the bioinformatics and logistical support to make the resource valuable and available to scientists. At the IGTC workshop held at UCSF in April, 2005, the membership of the IGTC agreed to centralize access to all publicly available gene trap lines by developing a user-oriented Website for the IGTC. It was further agreed that there should be a standardized annotation and identification to provide high confidence data for gene trap lines. The IGTC website, database, and annotation and identification pipeline are hosted by the RBVI. Hosting of the IGTC database and web site was transferred to the Mouse Biology Program at the University of California at Davis, effective October 1, 2010. Further information on this project is available at http://www.genetrap.org/

该子项目是利用资源的众多研究子项目之一由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持并且子项目的主要研究者可能是由其他来源提供的，包括其他 NIH 来源。子项目可能列出的总成本代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。随着人类和小鼠基因组序列的完成，人们对开发用于小鼠全基因组诱变的工具越来越感兴趣，其目标是在完整生物体的背景下分析基因组功能。基因捕获是一种高通量方法，可用于在小鼠胚胎干 (ES) 细胞的基因组中引入插入突变。基因捕获载体同时突变并报告插入位点内源基因的表达，并提供 DNA 标签以快速识别被破坏的基因。从携带基因陷阱插入的 ES 细胞系产生突变小鼠可应用于哺乳动物基因的大规模功能分析。国际 Trap 联盟 (IGTC) 由世界各地的实验室组成，共同创建突变鼠 ES 细胞系的公共库。这些细胞系可以由有兴趣在小鼠中产生报告标记的功能丧失突变的科学家在非合作的基础上获得。除了功能丧失之外，较新的基因捕获载体还提供各种插入后修饰策略，以允许生成其他实验等位基因。 IGTC的合作目标是生成代表小鼠基因组中所有或大部分基因的国际资源，并提供生物信息学和后勤支持，使该资源有价值并可供科学家使用。 2005 年 4 月在加州大学旧金山分校举行的 IGTC 研讨会上，IGTC 成员同意通过为 IGTC 开发一个面向用户的网站来集中访问所有公开可用的基因陷阱系。进一步同意应该有一个标准化的注释和鉴定，为基因陷阱系提供高可信度的数据。 IGTC 网站、数据库以及注释和识别管道由 RBVI 托管。 IGTC 数据库和网站的托管权已转移至加州大学戴维斯分校的小鼠生物学项目，自 2010 年 10 月 1 日起生效。有关该项目的更多信息，请访问 http://www.genetrap.org/