HALIOTIS DIVERSICOLOR HEMOCYANIN

杂色鲍 血蓝蛋白

• 批准号: 8361099
• 负责人: Qing-Yu Zhang
• 金额: $ 2.45万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361099
• 关键词: AIDS Vaccines; Active Sites; Affinity; Allosteric Regulation; Amino Acid Sequence; Arthropods; Binding; Complex; Copper; Diagnostic; Electron Microscopy; Funding; Grant; Haptens; Hemocyanin; Hemolymph; Human; Immune; Immunotherapeutic agent; Location; Malignant Neoplasms; Mammals; Maps; Morphology; National Center for Research Resources; Oxygen; Principal Investigator; Property; Reaction; Research; Research Infrastructure; Resources; Series; Source; Structure; United States National Institutes of Health; cost; interest; macromolecular assembly; macromolecule; microbial; molecular assembly/self assembly; molecular mass; oxygen transport; polypeptide; respiratory protein; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The hemocyanins are copper-containing respiratory proteins often present as large molecular assemblies in the hemolymph of many mollusks and arthropods. Besides the function of transporting oxygen, hemocyanin can be an effective immune-stimulant in mammals (including humans). For example, the hemocyanin of the giant keyhole limpet Megathura crenulata finds application as an immunotherapeutic agent for the treatment of certain cancers (Helling et al., 1994; Helling et al., 1995), and is used as diagnostic tool for bilharziosis and as a hapten carrier for AIDS vaccine (Naylor et al., 1991). Another perspective is that hemocyanins are involved in immune reaction to microbial challenge of mollusks and arthropods (Destoumieux-Garzon et al., 2001). A number of molluscan and arthropod hemocyanins are being investigated in terms of morphology, amino acid sequence, subunit composition, and structure. The subunit of molluscan hemocyanins is a polypeptide of ~400 kDa, folded into a series of seven or eight globular substructures, the so-called 'functional units' (FUs). Such a FU has a molecular mass of 45-65 kDa and carries a single binuclear copper active site. Allosteric regulation and cooperativity of hemocyanin subunits (as in arthropods) or FUs (as in mollusks) are responsible for the high oxygen affinity of the whole hemocyanin macromolecular assembly. Zhang's lab is interested in the structure properties of whole hemocyanin bound with hapten as an immune-stimulant. Difference maps of the apo-hemocyanin and hemocyanin-hapten complex would provide the location of such binding

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 血蓝蛋白是含铜的呼吸蛋白，通常以大的形式存在 许多软体动物和节肢动物的血淋巴中的分子组装。除了 血蓝蛋白具有运输氧气的功能，可以作为有效的免疫刺激剂 哺乳动物（包括人类）。例如，巨型匙孔帽贝的血蓝蛋白 Megathura crenulata 作为免疫治疗剂用于治疗 某些癌症（Helling 等人，1994；Helling 等人，1995），并用作诊断工具 用于血吸虫病和作为艾滋病疫苗的半抗原载体（Naylor 等，1991）。其他 观点认为，血蓝蛋白参与对微生物挑战的免疫反应 软体动物和节肢动物（Destoumieux-Garzon 等，2001）。 一些软体动物和节肢动物血蓝蛋白正在被研究 形态、氨基酸序列、亚基组成和结构。的亚基为 软体动物血蓝蛋白是一种约 400 kDa 的多肽，折叠成一系列七个或多个 八个球状子结构，即所谓的“功能单元”（FU）。这样的 FU 有一个 分子量为 45-65 kDa，并带有单个双核铜活性位点。变构 血蓝蛋白亚基（如节肢动物）或 FU（如 软体动物）负责整个血蓝蛋白的高氧亲和力 大分子组装。 张的实验室对与结合的整个血蓝蛋白的结构特性感兴趣 半抗原作为免疫刺激剂。脱辅基血蓝蛋白和脱辅基血蓝蛋白的差异图 血蓝蛋白-半抗原复合物将提供这种结合的位置