VESOSOME

血管体

• 批准号: 8361088
• 负责人: Joseph Anthony Zasadzinski
• 金额: $ 0.49万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361088
• 关键词: 6-carboxyfluorescein; Clinical; Drug Carriers; Electron Microscopy; Encapsulated; Funding; Gold; Grant; High temperature of physical object; Lasers; Lecithin; Lipid Bilayers; Lipids; Liposomes; Membrane; Nanosphere; National Center for Research Resources; Pharmaceutical Preparations; Phase; Physiologic pulse; Preparation; Principal Investigator; Research; Research Infrastructure; Resources; Source; Structure; Therapeutic Agents; Transmission Electron Microscopy; United States National Institutes of Health; Vesicle; aqueous; biological systems; carboxyfluorescein; cold temperature; copolymer; cost; macromolecule; particle; response; saturated fat

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Vesosome, a new multi-compartment structure consisting of drug-loaded liposomes encapsulated within another bilayer, is a promising drug carrier with better retention and stability compared with unilamellar liposomes. The current vesosome preparation takes advantage of the interdigitated phase of saturated lipids, which causes lipid bilayers to form flat, open sheets at low temperature, that close to form large unilamellar vesicles at higher temperatures. During this closure, the interdigitated sheets encapsulate other lipid vesicles or colloidal particles to become the outer membrane of the vesosome. It has been shown that by adding the triblock copolymer to the interdigitated sheets made from dipalmitol-phosphatidylcholine, the vesosomes achieve the proper size for clinical use. Meanwhile, hollow gold nanospheres with absorbance wavelength in the visible and near infrared (NIR) range are good candidates to trigger the release of contents from drug carriers. Vesosomes containing hollow gold nanospheres in their aqueous interior compartments have been verified by cryo electron transmission microscopy. Furthermore, 6-carboxyfluorescein has been encapsulated together with gold spheres to investigate the release from vesosomes irradiated with femetosecond laser pulses. Substantial amounts of carboxyfluorescein were released in response to only a few laser pulses. Our results suggest vesosomes containing hollow gold nanospheres, with large loading capacity, are useful for laser induced delivery of therapeutic agents and other applications of lasers in biological systems.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 Vesosome是一种新型多室结构，由封装在另一个双层内的载药脂质体组成，是一种有前途的药物载体，与单层脂质体相比具有更好的保留和稳定性。目前的囊泡制剂利用了饱和脂质的叉指相，这导致脂质双层在低温下形成平坦、开放的薄片，在较高温度下接近形成大的单层囊泡。在此闭合过程中，叉指片封装其他脂质囊泡或胶体颗粒，成为囊泡的外膜。已经表明，通过将三嵌段共聚物添加到由二棕榈醇-磷脂酰胆碱制成的叉指片中，囊泡达到了临床使用的适当尺寸。同时，吸收波长在可见光和近红外（NIR）范围内的空心金纳米球是触发药物载体释放内容物的良好候选者。已通过冷冻电子透射显微镜验证了其水性内部隔室中含有空心金纳米球的囊泡。此外，6-羧基荧光素已与金球封装在一起，以研究飞秒激光脉冲照射下的囊泡的释放情况。仅响应几个激光脉冲就释放出大量的羧基荧光素。我们的结果表明，含有空心金纳米球的囊泡具有较大的负载能力，可用于激光诱导治疗剂的输送以及激光在生物系统中的其他应用。