Transgenic Mice for Hybrid Voltage Sensor Imaging of Neural Circuitry

用于神经回路混合电压传感器成像的转基因小鼠

基本信息

批准号：
8675971
负责人：
MEYER B. JACKSON
金额：
$ 22.15万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-06-15 至 2015-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8675971
关键词：
Action Potentials Animals Axon Biological Neural Networks Brain Brain region Cell Count Cells Charge DNA Data Dendrites Development Electroporation Fluorescence Fluorescence Microscopy Fluorescence Resonance Energy Transfer Functional disorder Genetic Techniques Green Fluorescent Proteins Growth Associated Protein 43 Hybrids Image Laboratories Learning Lipid Bilayers Maps Membrane Membrane Potentials Memory Methods Movement Mus N-terminal Neurologic Neurons Neurosciences Research Optics Pattern Perception Performance Population Proteins Reporter Reporting Research Personnel Signal Transduction Slice Stimulus Techniques Testing Transgenic Mice Transgenic Organisms Work base brain cell cell type dipicrylamine improved in utero interest neural circuit neuronal cell body patch clamp promoter public health relevance relating to nervous system research study sensor tool voltage

项目摘要

DESCRIPTION (provided by applicant): The hybrid voltage sensor (hVOS) technique enables researchers to use green fluorescent protein and other fluorescent proteins to image membrane potential. Because hVOS probes are encoded by DNA, genetic techniques can be used to target them to specific types of cells. This project will develop mice with hVOS probes targeted to specific types of neurons in the mouse brain. Animals will be developed with hVOS probes expressed in a wide variety of brain regions and cell types. Animals will also be developed that can be used to target very specific populations of cells. Slices of brain prepared from these animals will be very useful in imaging experiments. Essentially any brain region and any nerve cell type will be accessible to studies using the strategy advanced in this work. With these animals researchers will be able to study the electrical activity of large numbers of cells simultaneously. A hVOS probe developed in this laboratory was found to preferentially target axons. Animals expressing this hVOS probe will be useful in general studies of axonal excitability.

描述（由申请人提供）：混合电压传感器（HVOS）技术使研究人员能够使用绿色荧光蛋白和其他荧光蛋白来对膜电位进行成像。由于HVOS探针是由DNA编码的，因此遗传技术可用于将其靶向特定类型的细胞。该项目将使用针对小鼠大脑中特定类型的神经元的HVO探针发展小鼠。动物将使用在各种大脑区域和细胞类型中表达的HVO探针开发。还将开发动物，可用于靶向非常特定的细胞群体。从这些动物制备的大脑切片将在成像实验中非常有用。从本质上讲，使用这项工作中提出的策略，可以使用任何大脑区域和任何神经细胞类型。借助这些动物，研究人员将能够同时研究大量细胞的电活动。发现该实验室中开发的HVO探针优先靶向轴突。表达此HVO探针的动物将在轴突兴奋性的一般研究中有用。