The Oral Microbiome in HIV-associated Oral Warts and Candidiasis

HIV 相关口腔疣和念珠菌病中的口腔微生物组

• 批准号: 8485362
• 负责人: PAUL L FIDEL
• 金额: $ 50.52万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-21 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8485362
• 关键词: Affect; Age; Anti-Retroviral Agents; Bacteria; CD4 Lymphocyte Count; CD4 Positive T Lymphocytes; Candida; Candida albicans; Candidiasis; Cell Count; Clinical Research; Communities; Contracts; Cross-Sectional Studies; Data; Death Rate; Dental caries; Diabetes Mellitus; Diagnostic; Disease; Ecology; Functional disorder; Genetic; Goals; HIV; HIV therapy; Head and Neck Cancer; Head and Neck Squamous Cell Carcinoma; Health; Health Status; Heart Diseases; Human Papillomavirus; Immunosuppression; Individual; Infection; Lead; Left; Lipids; Methodology; Mouth Diseases; Mucous Membrane; Opportunistic Infections; Oral; Oral Manifestations; Oral candidiasis; Oral cavity; Oral health; Pathogenesis; Patients; Periodontal Diseases; Play; Predisposition; Prevalence; Prognostic Marker; Race; Reporting; Risk; Risk Factors; Role; Salivary Glands; Sequence Analysis; Site; Smoking; Syndrome; Technology; Testing; Virus Diseases; antiretroviral therapy; base; cost; expectation; falls; frontier; high risk; innovation; malignant mouth neoplasm; microbial; microbial community; microbiome; novel; oral microbiome; oral wart; oropharyngeal thrush; pathogen; prevent; public health relevance; pyrosequencing; rRNA Genes; sex; therapeutic target; therapy development; tool

DESCRIPTION (provided by applicant): Human immunodeficiency virus (HIV) has infected over 30 million individuals worldwide. Left untreated, over 50% of these individuals will incur an oral manifestation associated with HIV. Currently, the most common oral manifestations of HIV disease are oropharyngeal candidiasis (OPC) and oral warts. In addition, HPV-associated head and neck squamous cell carcinomas (HNSCC) are increased in HIV+ individuals and have not seen a reduction in prevalence with the advent of anti-retroviral therapy (ART). The factors which determine an HIV+ individual's predisposition to OPC, HPV and other oral manifestations are poorly understood as well as how treatments for HIV influence the oral cavity and/or infections/diseases. A realistic description of the composition and diversity of the oral microflora (microbiome) is essential to understanding health and disease in the oral cavity. However, how the oral microbiome is impacted by HIV has not been described, but based on preliminary data, is expected to be significant. Moreover, ART used to treat HIV also likely impacts/alters the oral microbiome. We will use PCR-amplification of 16S rRNA genes and massive parallel sequencing to test the hypothesis that both HIV infection and ART alter the composition and diversity of bacterial species in the oral cavity and such composition can be associated with risk of oral manifestations such as HIV-associated OPC and ART-associated oral warts and HNSCC.

描述（由申请人提供）： 人类免疫缺陷病毒（HIV）已感染了全球超过3000万个人。剩下的未经治疗，超过50％的人会引起与艾滋病毒相关的口服表现。目前，HIV疾病最常见的口服表现是口咽念珠菌病（OPC）和口腔疣。此外，HIV+个体中与HPV相关的头部和颈部鳞状细胞癌（HNSCC）有所增加，并且随着抗逆转录病毒疗法（ART）的出现，患病率降低。确定艾滋病毒+个人对OPC，HPV和其他口服表现的易感性的因素对艾滋病毒的治疗方式较少，以及如何影响口腔腔和/或感染/疾病。现实的描述 口腔菌群（微生物组）的组成和多样性对于理解口腔健康和疾病至关重要。但是，尚未描述口服微生物组受到HIV的影响，但基于初步数据，预计将是重要的。此外，用来治疗艾滋病毒的艺术也可能影响/改变口服微生物组。我们将使用16S rRNA基因和大量平行测序的PCR放大，以测试以下假设：HIV感染和艺术都会改变口腔中细菌物种的组成和多样性以及这种组成可能与HIV相关的OPC和艺术相关的口服和艺术相关的口腔相关的口服表现的风险有关。