Identifying molecular targets and biological signatures of CAM natural products

识别 CAM 天然产物的分子靶标和生物特征

基本信息

批准号：
8675802
负责人：
CHUO CHEN
金额：
$ 43.2万
依托单位：
UT SOUTHWESTERN MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-08-01 至 2016-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8675802
关键词：
Adverse effects Affinity Affinity Chromatography African Anti Inflammatory Analgesics Antioxidants Arthritis Benefits and Risks Binding Biochemical Biological Biological Assay Biological Factors Cardiovascular system Cells Chemicals Claw Clinical Clinical Trials Complementary DNA Complementary and alternative medicine Complex Development Drug usage Ensure Family Folk Medicine Future Goals Grapes Health Health Benefit Human Investigation Knowledge Label Measures Methodology Methods Modification Molecular Molecular Target National Center for Complementary and Alternative Medicine Pathway interactions Peptide Initiation Factors Pharmaceutical Preparations Play Property Proteins Proteomics Reporting Research Resveratrol Rheumatism Risk Role Skin Work anti aging base cell type chronic pain design dietary supplements human FRAP1 protein improved in vivo interest novel polyphenol programs protein profiling response small molecule tool

项目摘要

DESCRIPTION (provided by applicant): Many natural product extracts have been widely used as complementary and alternative medicines (CAM). However, their health benefits and risks have not been subjected to rigorous study, nor have their mechanisms of action been fully understood. The long-term goal of our research program is to validate the medicinal effects of these CAM natural products. The focus of this proposed research is to develop assays that will allow us to identify the molecular targets of CAM natural products of high priority to the NCCAM, and to broadly capture the signatures of biological activities indicative of their complex medicinal effects. Both chemical and biological tools will be used. For the target identification component, we will develop proteomic and cDNA strategies based on both labeled and label-free target identification methods to identify the direct binding targets of resveratrol and Devil's claw, two classes of widely used CAMs. For the signature component, we will use protein profiling to assess the broad biological effects and pharmaco-response pathways of CAM natural products, and develop specific assays and probes to report on the signature response proteins of CAM natural products, thus measuring their in vivo efficacy and facilitating the design of future clinical trials.

描述（由申请人提供）：许多天然产物提取物已被广泛用作补充和替代药物（CAM）。然而，它们的健康益处和风险尚未经过严格的研究，其作用机制也尚未得到充分了解。我们研究计划的长期目标是验证这些 CAM 天然产品的药用效果。这项拟议研究的重点是开发检测方法，使我们能够识别 NCCAM 高度重视的 CAM 天然产物的分子靶标，并广泛捕获表明其复杂药用效果的生物活性特征。将使用化学和生物工具。对于目标识别部分，我们将开发基于标记和无标记目标识别方法的蛋白质组和 cDNA 策略，以识别白藜芦醇和魔鬼爪（两类广泛使用的 CAM）的直接结合目标。对于特征成分，我们将使用蛋白质分析来评估 CAM 天然产物的广泛生物效应和药物反应途径，并开发特定的检测方法和探针来报告 CAM 天然产物的特征反应蛋白，从而测量其体内功效并促进未来临床试验的设计。