Resolving orofacial neuropathic pain evoked by compression of a trigeminal nerve branch using rationally integrated complementary approaches

使用合理整合的补充方法解决三叉神经分支受压引起的口面部神经性疼痛

• 批准号: 9703533
• 负责人: WOLFGANG B. LIEDTKE
• 金额: $ 30.51万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-20 至 2021-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9703533
• 关键词: Affect; Affective; Afferent Neurons; American; Behavior; Biological Assay; Blood Cells; Body Regions; Brain; Brain Stem; Calcium; Chronic; Complement; Complementary and alternative medicine; Cornea; Data; Dinoprostone; Disease; Effectiveness; Electroacupuncture; Emotional; Enzyme-Linked Immunosorbent Assay; Female; Goals; Human; Hypersensitivity; Image; Implant; Incidence; Inflammation; Inflammatory; Intake; Lateral; Light; Measures; Meninges; Mus; Nerve; Nerve Pain; Neuroglia; Neuronal Plasticity; Neurons; Organ; Pain; Pathway interactions; Patients; Peripheral nerve injury; Persistent pain; Plant Roots; Population; Quality of life; Resolution; Self-Injurious Behavior; Sensory; Serum; Sinus; Site; Stress; Structure; Structure of trigeminal ganglion; Structure of trigeminal nerve spinal tract nucleus; Swimming; Temporomandibular Joint; Testing; Time; Tooth structure; Trigeminal Pain; Trigeminal System; Trigeminal nerve structure; Tumor-infiltrating immune cells; Victimization; Work; base; behavior measurement; behavioral response; chemokine; chronic neuropathic pain; chronic pain; clinical practice; constriction; craniofacial; cytokine; effectiveness testing; experimental study; improved; in vivo; lipid mediator; macrophage; mouse model; nerve injury; neural circuit; neuroinflammation; non-opioid analgesic; orofacial; painful neuropathy; parabrachial nucleus; response; satellite cell; sensory stimulus; sex; stem; vocalization

Project 1: Resolving orofacial neuropathic pain evoked by compression of a trigeminal nerve branch using rationally integrated complementary approaches ABSTRACT Neuropathic pain in the craniofacial region innervated by trigeminal sensory neurons affects approximately at least 1x106 Americans. The goal of this project is to assess efficiency and elucidate key mechanisms of rationally-combined complementary approaches to treat trigeminal neuropathic pain. The central hypothesis of Project-1 is that natural pro-resolution lipid mediators (specialized pro-resolution lipid mediators, SPMs) or auricular electro-acupuncture (aEA) can produce beneficial effects in alleviating orofacial neuropathic pain, aggravated by stress, by modulating neuroinflammation and neural plasticity. We will use a well-established mouse model of trigeminal neuropathic pain, chronic constriction of the infraorbital nerve (CCI-ION), to test our central hypothesis. We will first define the time course of neuropathic pain and inflammatory and neuroinflammatory profiles in response to CCI-ION in both sexes with and without stress. Subsequently, we will determine the effects of aEA and SPMs on pain-related behaviors as well as resolution of inflammatory and neuroinflammatory profiles evoked by CCI-ION. Finally, we will explore effectiveness of aEA and SPMs on reducing spontaneous activity of trigeminal sensory neurons and of ongoing pain-activated CNS neurons after CCI-ION.

项目1：通过压缩三叉神经分支引起的口面神经性疼痛解决 使用合理整合的互补方法 抽象的 三叉神经元神经支配的颅面区域的神经性疼痛大致影响 至少1x106美国人。该项目的目的是评估效率并阐明关键机制 理性综合的互补方法治疗三叉神经性疼痛。中心假设 Project-1的是自然的促分辨率脂质介质（专业的促分辨率脂质介质，SPMS）或 耳神经针灸（AEA）可以减轻口腔神经性疼痛，产生有益的作用， 通过调节神经炎症和神经可塑性来加剧压力。我们将使用良好的 三叉神经性疼痛的小鼠模型，胸骨神经的慢性收缩（CCI-ION）进行测试 我们的中心假设。我们将首先定义神经性疼痛和炎症性的时间过程 在有和没有压力的男女中，神经炎症谱响应CCI-ION。随后，我们 将确定AEA和SPM对疼痛相关行为的影响以及炎症的分辨率 和CCI-ION引起的神经炎性谱。最后，我们将探索AEA和SPM的有效性 关于降低三叉神经元和持续疼痛激活CNS神经元的自发活性 CCI-ION之后。