Molecular Core

分子核心

• 批准号: 9703532
• 负责人: SVEN-ERIC JORDT
• 金额: $ 31.23万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-20 至 2021-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9703532
• 关键词: Acupuncture Points; Analgesics; Astrocytes; Behavioral; Biological Markers; Blinded; Blood; Blood Circulation; Brain; Brain Stem; Cell Culture Techniques; Cells; Cerebrospinal Fluid; Chronic; Core Facility; Dependovirus; Detection; Ear; Enzyme-Linked Immunosorbent Assay; Evaluation; Experimental Designs; Feedback; Flow Cytometry; Fracture; Gene Expression; Gene Expression Profiling; Genes; Genetic Transcription; Guidelines; Immunoassay; Immunologics; Individual; Inflammation; Inflammatory; Injury; Intervention; Label; Leukocytes; Liquid substance; Maps; Measures; Methods; Modeling; Molecular; Mus; Neurons; Neuropeptides; Nociceptors; Organ; Pain; Pathway interactions; Peripheral; Persistent pain; Population; Postoperative Pain; Production; RNA; Recovery; Research Personnel; Resolution; Resources; Sampling; Sensory Ganglia; Site; Spinal; Spinal Cord; Stimulus; Stress; Subfamily lentivirinae; Syndrome; Therapeutic; Therapeutic Intervention; Tibial Fractures; Tissue Sample; Tissues; Tracer; Transcript; Trigeminal Pain; Trigeminal System; Variant; Viral; Virus Diseases; Visual; Work; afferent nerve; chemokine; chemotherapy; cytokine; data exchange; injured; lipid mediator; lipoxin A4; nerve injury; neuroinflammation; neuronal circuitry; pain model; pain signal; tool

Molecular Core – Summary The molecular and immunological characterization of inflammation and injury markers is crucial for the evaluation of the analgesic complementary approaches proposed in all four projects of this Center. Preliminary studies have identified sets of pro-inflammatory genes in sensory ganglia, spinal cord, brainstem and brain strongly induced by the pro-algesic stimuli in the models (chemotherapy, tibial fracture, functional pain syndrome, trigeminal pain) suggesting that pain has detrimental effects on the function of the CNS and induces inflammatory states in other organs remote from the site of injury. The Molecular Core will support the Center Projects through the following Specific Aims: Core Specific Aim 1: Analyze the effects of injuries, prior stress and complementary analgesic interventions on gene expression in injured tissues and neuronal tissues of the pain pathway. Core Specific Aim 2: Determine systemic and tissue levels of inflammatory biomarkers and inflammation-resolving lipid mediators. Core Specific Aim 3: Examine the effects of pain stimuli, prior stress and complementary interventions on systemic leukocyte profiles and neuroinflammatory cells in neuronal tissues. Core Specific Aim 4:Provide resources for production of viral tracers for labeling of neuronal circuits engaged in pain models and by bioelectronic complementary analgesic interventions. Methods include quantitative PCR analysis, multiplex immunoassays and ELISAs, flow cytometry and cell culture. The Molecular Core will work closely together with Project Investigators, Behavioral Core, the Statistical Unit of the Administrative Core and external core facilities to establish a workflow for sample tracing, blinded data transfer and feedback for optimization of experimental designs.

分子芯 - 摘要 感染和损伤标记的分子和免疫学表征对于评估至关重要 在该中心的所有四个项目中提出的镇痛完成者的方法中。初步研究 在感觉神经节，脊髓，脑干和大脑中鉴定的促炎基因集 通过模型中的促脂刺激（化学疗法，胫骨骨折，功能性疼痛综合征，三叉神经疼痛） 表明疼痛对中枢神经系统的功能有害，并在其他 器官远离受伤部位。分子芯将通过以下来支持中心项目 具体目的：核心特定目标1：分析伤害，先前的压力和完全镇痛的影响 疼痛途径的受伤组织和神经元组织中基因表达的干预措施。特定于核心 目标2：确定炎症生物标志物和炎症脂质的全身和组织水平 调解人。核心特定目标3：检查疼痛刺激，先前的压力和完整性的影响 神经元组织中系统性白细胞谱和神经炎性细胞的干预。特定于核心 目标4：提供用于生产病毒示踪剂的资源，用于标记参与疼痛模型的神经元电路 并通过生物电子完整的镇痛干预措施。方法包括定量PCR分析， 多重免疫测定和ELISA，流式细胞仪和细胞培养。分子芯将密切工作 与项目调查人员，行为核心，行政核心的统计单元和外部的统计单元 核心设施，以建立用于样本跟踪，盲目数据传输和反馈的工作流程，以优化 实验设计。