Sensitive Triple Quadrupole Mass Spectrometer for Translational Research

用于转化研究的灵敏三重四极杆质谱仪

• 批准号: 8247350
• 负责人: Nichole A Reisdorph
• 金额: $ 44.32万
• 依托单位: NATIONAL JEWISH HEALTH
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-19 至 2013-05-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247350
• 关键词: 5 year old; Address; Amino Acid Sequence; Basic Science; Biological Assay; Biomedical Research; Budgets; Clinical; Commit; Complex; Development; Diagnostic; Equipment; Event; Funding; Health; Institution; Maintenance; Mass Spectrum Analysis; Medicine; Occupational activity of managing finances; Peptide Sequence Determination; Peptides; Phosphorylation; Pilot Projects; Proteins; Reagent; Research; Research Personnel; Research Project Grants; Resources; Sampling; System; Technology; Translational Research; United States National Institutes of Health; Wages; clinical assay development; follower of religion Jewish; innovation; instrument; instrumentation; mass spectrometer; mass spectrometry shared resource; multiple reaction monitoring; respiratory; small molecule; success

DESCRIPTION (provided by applicant): This application is for a triple quadrupole (QQQ) mass spectrometer system for the purpose of performing translational and basic science applications within the Mass Spectrometry Facility at National Jewish Health (NJH). The instrument will be used to develop and conduct high- throughput, single- and multi-plex assays using multiple reaction monitoring (MRM) in order to quantitate small molecules and peptides (proteins) from a variety of biofluids and complex samples. National Jewish Health has committed almost $1,000,000 (not including equipment) in the past 4 years towards the development of a state-of-the-art mass spectrometry facility; these technologies are an essential component of research for over 25 investigators at NJH whose long-term success is dependent on mass spectrometry. This proposal highlights 9 projects whose success depends specifically on the acquisition of the requested instrument. These include quantitative protein sequencing, quantitation of phosphorylation events, and clinical assay development. Our objective is to continue to provide our investigators with a shared mass spectrometry resource to support current and proposed NIH-funded biomedical research and NJH has committed substantial resources towards this end. Instrumentation in the facility is now five years old and no longer adequate to the needs of our investigators; we are taking steps to maintain a state-of-the-art facility, including acquisition of new instruments. This proposal will specifically demonstrate the following components: 1) a substantial, justified need for a QQQ mass spectrometer, 2) existing expertise to operate and oversee the instrument, 3) significant enhancement of NIH-funded research projects, 4) a practical financial and management plan, and 5) a strong institutional commitment. The NJH mass spectrometry facility addresses the tremendous demand for state-of-the-art technologies by our NIH-funded researchers as well as for innovative pilot studies and the development of clinical diagnostics. The requested QQQ will be an essential component of this facility. NJH supports the facility through an annual budget of $250,000, including salary, maintenance, and reagents. As a highly focused institution, we believe that NJH is poised to deliver innovative, high-impact studies in respiratory medicine. This can only be achieved if cutting edge technologies remain available to our investigators. PUBLIC HEALTH RELEVANCE: A successful application will have a significant impact on public health by supporting researchers who are currently engaged in respiratory and immunological research. The instrument will be used to support studies where there is the potential to validate candidate biomarkers for disease, identify drug targets, and improve our overall knowledge of the basic mechanisms of several diseases.

描述（由申请人提供）：本申请适用于三重四极杆 (QQQ) 质谱仪系统，用于在国家犹太健康中心 (NJH) 的质谱设施内执行转化和基础科学应用。该仪器将用于使用多重反应监测（MRM）开发和进行高通量、单重和多重测定，以便定量各种生物流体和复杂样品中的小分子和肽（蛋白质）。国家犹太健康中心在过去 4 年里投入了近 1,000,000 美元（不包括设备）用于开发最先进的质谱设施；这些技术是 NJH 超过 25 名研究人员研究的重要组成部分，他们的长期成功依赖于质谱分析。该提案重点介绍了 9 个项目，这些项目的成功具体取决于所需仪器的获取。其中包括定量蛋白质测序、磷酸化事件定量和临床检测开发。我们的目标是继续为我们的研究人员提供共享的质谱资源，以支持当前和拟议的 NIH 资助的生物医学研究，并且 NJH 已为此投入了大量资源。该设施中的仪器现已使用五年，不再足以满足我们调查人员的需求；我们正在采取措施维持最先进的设施，包括购置新仪器。该提案将具体展示 以下组成部分：1) 对 QQQ 质谱仪的大量合理需求，2) 操作和监督该仪器的现有专业知识，3) NIH 资助的研究项目的显着增强，4) 实用的财务和管理计划，以及 5)强有力的机构承诺。 NJH 质谱设施满足了 NIH 资助的研究人员对最先进技术以及创新试点研究和临床诊断开发的巨大需求。所请求的 QQQ 将是该设施的重要组成部分。 NJH 通过 250,000 美元的年度预算支持该设施，包括工资、维护和试剂。作为一个高度专注的机构，我们相信 NJH 已准备好在呼吸医学领域开展创新、高影响力的研究。只有当我们的研究人员仍然可以使用尖端技术时，才能实现这一目标。 公共健康相关性：成功的应用将为目前从事呼吸和免疫学研究的研究人员提供支持，从而对公共健康产生重大影响。该仪器将用于支持有潜力验证疾病候选生物标志物、确定药物靶点并提高我们对几种疾病基本机制的整体了解的研究。

项目成果

Determining the presence of asthma-related molecules and salivary contamination in exhaled breath condensate.

确定呼出气冷凝液中是否存在哮喘相关分子和唾液污染。

• 发表时间: 2017-04-12
• 影响因子: 5.8
• 作者: Cruickshank;Armstrong, Michael;Powell, Roger;Gomez, Joe;Elie, Marc;Reisdorph, Nichole
• 通讯作者: Reisdorph, Nichole

Lipid mediators are detectable in the nasal epithelium and differ by asthma status in female subjects.

脂质介质可在鼻上皮中检测到，并且因女性受试者的哮喘状态而异。

• 发表时间: 2022-10
• 作者: Johnson, Randi K;Manke, Jonathan;Campbell, Monica;Armstrong, Michael;Boorgula, Meher Preethi;Pinheiro, Gabriela;Santana, Cinthia Vila Nova;Mathias, Rasika A;Barnes, Kathleen C;Cruz, Alvaro;Reisdorph, Nichole;Figueiredo, Camila A
• 通讯作者: Figueiredo, Camila A

Quantitation of peptides from non-invasive skin tapings using isotope dilution and tandem mass spectrometry.

使用同位素稀释和串联质谱法对非侵入性皮肤贴带中的肽进行定量。

• DOI: 10.1016/j.jchromb.2018.03.031
• 发表时间: 2018-05-01
• 期刊: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
• 作者: Reisdorph N;Armstrong M;Powell R;Quinn K;Legg K;Leung D;Reisdorph R
• 通讯作者: Reisdorph R