Systemic responses to tissue damage and regeneration

对组织损伤和再生的系统反应

基本信息

批准号：
8716780
负责人：
MEGUMI M. FUSE
金额：
$ 15.4万
依托单位：
SAN FRANCISCO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-08-01 至 2016-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8716780
关键词：
Ablation Address Adult Agonist Animal Model Animals Area Biological Assay Biological Metamorphosis Biological Models Blood Body Size Cells Chronic Collaborations Coupled Crustacea Cystic Fibrosis Data Development Developmental Biology Developmental Delay Disorders Digit structure Disease Drosophila genus Drosophila melanogaster Ecdysteroids Endocrine Endocrine system Endocrinology Environment Enzyme-Linked Immunosorbent Assay Epithelial Flow Cytometry Genetic Genetic Techniques Goals Growth Hemocytes Hemolymph High Pressure Liquid Chromatography Hormones Human Immune Immune system Immunology Inflammation Inflammatory Injection of therapeutic agent Injury Insecta Insulin Invertebrates Laboratories Larva Learning Manduca Manduca sexta Marines Measures Mentors Mentorship Modeling Molecular Biology Molecular Genetics Monitor Natural regeneration Operative Surgical Procedures Organ Peripheral Phenotype Pilot Projects Population Primordium Process Puberty Publications Pupa Research Retinoids Role Sampling Signal Transduction Stress Structure Technical Expertise Techniques Time Tissues Universities Wing Wood material cancer therapy environmental stressor imaginal disc immunocytochemistry in utero interest irradiation meetings regenerative release factor repaired response tissue regeneration tissue repair

项目摘要

DESCRIPTION (provided by applicant): The current goals of this research are to characterize the systemic responses to tissue regeneration after injury in insects, such as delays in developmental that provide time for the repair of select tissues. Many tissues display an impressive ability for repair and regeneration after injury. This is seen in invertebrate and vertebrate tissues and even in digit replacement in humans in utero. Compensatory growth and regeneration are vital processes used to replace damaged tissue during animal development. Responses to tissue injury and regeneration include inflammation or reduced growth in neighboring cells, as well as changes in growth rate including delays in development. Much is known of the local responses to tissue damage, but there is a gap in information on how local signals produce systemic immune and developmental responses. Insect imaginal discs, the precursors to adult structures such as wings, show exceptional regenerative capabilities, where they secrete signals that delay development when damaged. This project will (i) the characterize responses of the immune system after damage to the imaginal discs, (ii) identify and characterize the factors released from the damaged discs providing signals to the endocrine system, and (ii) determine the actions of these blood-borne factors on the endocrine system. This research will be conducted on the larger insect, Manduca sexta, as a means of developing a complementary model organism to the genetically tractable, but smaller fruitfly, Drosophila melanogaster. Given the mentorship by Dr. Hariharan of UC Berkeley, this pilot project will broaden the scope of the PI's expertise in immunology and development for understanding how development is modulated in response to external stresses, and how tissues interact to monitor changes in growth and body size during development. It will also provide the learning environment for introducing Drosophila genetics to the lab when necessary.

描述（由申请人提供）：本研究的当前目标是表征昆虫受伤后组织再生的系统反应，例如发育延迟，为选定组织的修复提供时间。许多组织在受伤后表现出令人印象深刻的修复和再生能力。这在无脊椎动物和脊椎动物组织中，甚至在人类子宫内的手指替换中都可以看到。补偿性生长和再生是动物发育过程中用于替换受损组织的重要过程。对组织损伤和再生的反应包括炎症或邻近细胞生长减少，以及生长速率的变化，包括发育延迟。人们对组织损伤的局部反应了解很多，但关于局部信号如何产生全身免疫和发育反应的信息却存在空白。昆虫的成虫盘是成虫结构（如翅膀）的前体，表现出非凡的再生能力，它们在受损时会分泌延迟发育的信号。该项目将（i）表征成象盘受损后免疫系统的反应，（ii）识别并表征受损盘释放的因子，向内分泌系统提供信号，以及（ii）确定这些血液的作用影响内分泌系统的因素。这项研究将在较大的昆虫 Manduca sexta 上进行，作为开发一种与基因上易于驯化但较小的果蝇 Drosophila melanogaster 互补的模式生物的手段。在加州大学伯克利分校 Hariharan 博士的指导下，该试点项目将扩大 PI 在免疫学和发育方面的专业知识范围，以了解如何调节发育以应对外部压力，以及组织如何相互作用以监测生长和体型的变化在开发过程中。它还将为必要时将果蝇遗传学引入实验室提供学习环境。