Clinical Core

临床核心

基本信息

批准号：
8318893
负责人：
Baoxi Wang
金额：
$ 17.94万
依托单位：
UNIV OF MASSACHUSETTS MED SCH WORCESTER
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8318893
关键词：
Academy Address Aliquot Animals Antibodies Antigens Antimicrobial Resistance Bacterial Vaginosis Basic Science Biomedical Engineering Blocking Antibodies Blood Boston Cervical China Chinese People Chlamydia Chlamydia Infections Chlamydia trachomatis Clinic Clinical Clinical Research Collaborations Control Groups Data Dermatology Detection Discipline of Nursing Eligibility Determination Enrollment Epidemiology Epitopes Exposure to Faculty Female Genetic Variation Genital system Genotype Goals Gonorrhea Gram&apos s stain HIV Human Immune Immune response Immunity In Vitro Infection Infertility Institutes Irrigation Laboratories Laboratory Study Laboratory Technicians Lead Left Link Lipid A Measurement Measures Medical Medical center Methods Mycoplasma genitalium Names Natural Immunity Neisseria Neisseria gonorrhoeae Nested PCR Pap smear Paper Pelvic Inflammatory Disease Process Properdin Publishing Recruitment Activity Reporting Rice Role Scheme Science Scientist Serum Sexual Partners Sexually Transmitted Diseases Site Specimen Symptoms System Testing Time Training Training Programs Trichomonas vaginalis Urethra Urethritis Urine Vaccine Antigen Vaccines Vagina Vaginal Douching Variant Visit Woman adaptive immunity arm base complement pathway cytokine design experience genital secretion interest major outer membrane protein male man member men outreach porin prevent professor routine Bacterial stain symposium therapy development transmission process vaccine candidate

项目摘要

The Clinical Core Unit will provide support for three projects: Project 2 - Genetic variations in the innate immune response to Neisseria gononhoeae; Project 4 -The Altemative Complement Pathway (ACP) and Properdin in N. gonorrhoeae infection and Project 5 - Experimental and human protective immunity to N. gononhoeae infecfion. The core will recruit eligible subjects, collect and process serum specimens and cervico/lavage specimens from women and perfomn selected laboratory tests. The Clinical Core will: 1. Identify and enroll male subjects infected with N. gononhoeae and their female spread contacts. 2. Collect epidemiologic, clinical and laboratory data on female spread contacts at the Nanjing STD Clinic. 3. Collect, process and store gonococcal strains, blood and genital secrefions from women who are the sexpartners of a man wifii gonorrhea. 4. Perfonn PCR analysis for M. genitalium and 7. vaginalis and evaluate Gram's stains of vaginal secretions for BV (Nugent's criteria) to assess eligibility of female spread contacts to partidpate in a study of the role of naturally present vaccine antitrady (and blocking anfibody) in the acquisition of gonontiea. 5. Perform porin genotyping on gonococcal strains to validate transmission of like portypes between dually infected partners (Project 5). 6. Measure anfibody levels in blood, directed against candidate vaccine anfigens (2-1-L8 and 207 epitopes) and against the target (Rmp) of blocking antibodies in uninfected female spread contacts (Project 5). 7. Provide gonococcal strains and cervico/vaginal lavage specirr^ns from gononrhea infected female spread contacts (and uninfected contacts) to Project 2 for assessment of lipid A variations in sbains and for assessment of corresponding cytokine profiles in lavage specimens. 8. Provide cervico/vaginal lavage specimens to Project 4 to assess potential for ACP activation and the role of Properdin.

临床核心单元将为三个项目提供支持：项目 2 - 先天遗传变异对淋病奈瑟菌的免疫反应；项目 4 - 替代补体途径 (ACP) 和备解素在淋病奈瑟菌感染中的作用和项目 5 - 对淋病奈瑟菌的实验和人类保护性免疫淋病感染。核心将招募符合条件的受试者，收集和处理血清样本，从女性身上采集宫颈/灌洗标本并进行选定的实验室测试。临床核心将： 1. 识别并招募感染淋病奈瑟菌的男性受试者及其女性传播接触者。 2. 收集南京性病诊所女性传播接触者的流行病学、临床和实验室数据。 3. 收集、处理和储存女性性伴侣的淋球菌菌株、血液和生殖器分泌物一名男子 wifii 淋病的肖像。 4. 对生殖支原体和阴道支原体进行 PCR 分析并评估阴道分泌物的革兰氏染色 BV（纽金特标准）评估女性传播接触者参与角色研究的资格自然存在的疫苗反传统（和阻断抗体）在获得淋病中的作用。 5. 对淋球菌菌株进行孔蛋白基因分型，以验证双重菌株之间类似端口型的传播受感染的伙伴（项目 5）。 6. 测量血液中针对候选疫苗抗原（2-1-L8 和 207 表位）的抗体水平以及针对未感染女性传播接触者中阻断抗体的目标（Rmp）（项目5）。 7. 提供淋球菌菌株和淋病感染女性传播的宫颈/阴道灌洗标本项目 2 的接触者（和未感染的接触者），用于评估 sbains 中的脂质 A 变异以及评估灌洗标本中相应的细胞因子谱。 8. 向项目 4 提供宫颈/阴道灌洗标本，以评估 ACP 激活的潜力及其作用备解丁。