Exosome-mediated cell-cell signaling in Rett Syndrome

雷特综合征中外泌体介导的细胞信号传导

• 批准号: 8743295
• 负责人: HOLLIS T. CLINE
• 金额: $ 35.31万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-26 至 2017-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8743295
• 关键词: Address; Attention; Autistic Disorder; Biology; Brain; Cell Communication; Cell Culture Techniques; Cell Line; Cell model; Cells; Communication; Complex; Development; Dimensions; Disease; Event; Fathers; Fibroblasts; Harvest; Human; Lipids; Maintenance; Mediating; Membrane; Modeling; Mus; Nervous system structure; Neurodevelopmental Disorder; Neurons; Outcome; Patients; Play; Property; Proteins; Proteomics; Rett Syndrome; Role; Series; Signal Transduction; Synapses; Syndrome; Testing; Time; Vesicle; Work; base; cell type; extracellular; improved; induced pluripotent stem cell; intercellular communication; mind control; mouse model; nervous system disorder; neural circuit; novel; programs; public health relevance; relating to nervous system; research study

DESCRIPTION (provided by applicant): Exosome-mediated cell-cell communication in Rett Syndrome Development of functional neuronal circuits requires a complex series of events involving coordinated communication between multiple cell types over multiple dimensions of space and time. Extracellular membrane vesicles (EMVs), specifically exosomes, may serve as a novel form of cell-cell communication during brain development. Exosomes can transfer bioactive lipids, proteins, and RNAs between cells without requiring direct contact between the donor and recipient cells. So far, no studies have demonstrated that exosomes play a role in brain development or that deficits in exosome signaling might underlie neurodevelopmental disorders such as Rett Syndrome. We propose to address these fundamental open questions regarding the potential role of exosomes in brain circuit development in Rett Syndrome and healthy neurons using both mouse and hiPSC models.

描述（由申请人提供）：在功能性神经元电路的Rett综合征开发中，外泌体介导的细胞 - 细胞通信需要一系列复杂的事件，这些事件涉及在空间和时间的多个维度上，涉及多个细胞类型之间的协调通信。细胞外膜囊泡（EMV），特别是外泌体，可以用作脑发育过程中细胞 - 细胞通信的一种新型形式。外泌体可以在细胞之间传递生物活性脂质，蛋白质和RNA，而无需在供体和受体细胞之间直接接触。到目前为止，尚无研究表明，外泌体在大脑发育中起作用，或者外泌体信号的缺陷可能是神经发育疾病（例如RETT综合征）的基础。我们建议通过小鼠和HIPSC模型解决有关外泌体在RETT综合征和健康神经元中外泌体发育中潜在作用的基本开放问题。