Chromium VI-induced toxicity on Female Reproductive Function

六价铬对女性生殖功能的毒性

• 批准号: 8320185
• 负责人: Sakhila Banu
• 金额: $ 18.31万
• 依托单位: TEXAS A&M UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-16 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8320185
• 关键词: Address; Adverse effects; Antioxidants; Apoptosis; Apoptotic; Area; Ascorbic Acid; Birth; Blood; Bos taurus PARP protein; Breeding; Carcinogens; Cells; Child; Chromium; Cleaved cell; Cytochromes; Data; Delayed Puberty; Development; Environment; Environmental Pollution; Estrous Cycle; Exposure to; Failure; Female; Follicular Atresia; GDF9 gene; Gene Proteins; Generations; Goals; Health; Heavy Metals; Human; Hydrogen Peroxide; Industry; Infertility; Intervention; Knowledge; Lactation; Life; Lung; Mediating; Menstruation; Milk; Mission; Mitochondria; Molecular; Mothers; Mutagens; National Institute of Child Health and Human Development; National Institute of Environmental Health Sciences; Oocytes; Organ; Outcome; Ovarian; Ovary; Ovulation; Phosphorylation; Pregnancy; Pregnancy Outcome; Pregnancy Rate; Premature Labor; Prevention; Protein p53; Proteins; Proto-Oncogene Protein c-kit; Puberty; Rattus; Reproductive Health; Research; Solid; Steroid biosynthesis; Steroids; Supplementation; Testing; Therapeutic Intervention; Time; Tissues; Toxic effect; United States National Institutes of Health; Urine; Woman; Work; Working Women; base; bcl-xlong protein; bone morphogenetic protein 15; caspase-3; catalase; chromium hexavalent ion; experience; granulosa cell; granulose; innovation; member; neonate; novel; offspring; ovotoxicity; postnatal; pregnancy prevention; protective effect; pup; reproductive; reproductive function; response; toxicant

DESCRIPTION (provided by applicant): Environmental contamination with CrVI is a major threat to human health and has been increasing due to the wide range of industrial uses of chromium. Women working in Cr industries and living around Cr-contaminated environment experience various reproductive problems such as abnormal menses, infertility, still birth, which is accompanied by high Cr levels in their blood and urine. CrVI can be transferred through mother's milk to neonates. No therapeutic intervention strategy has been identified to protect reproductive health of women against adverse effects of CrVI-toxicity, because the underlying molecular and cellular mechanisms remain largely unknown. Our recent findings in rats demonstrate that lactation exposure to CrVI induces follicular atresia and apoptosis of granulose cells, decreases steroid genesis, delays puberty, and extends the estrous cycle; while vitamin C supplementation along with CrVI mitigates the adverse effect of CrVI, restores normal follicular development, and the onset of puberty in F1 generation. The objective of this application is to understand mechanisms through which lactational exposure to CrVI compromises ovarian function and pregnancy, and to evaluate prevention of vitamin C against CrVI-induced reproductive toxicity of F1 offspring. Our central hypothesis is that lactation exposure to CrVI induces apoptosis of granulosa cells and follicular atresia, decreases ovulation, compromises pregnancy, and induces preterm labor in the F1 female offspring; and vitamin C mitigates CrVI-induced reproductive toxicity. Specific Aim-1 will determine the molecular mechanisms through which lactational exposure to CrVI induces ovarian follicular atresia in F1 offspring, and to evaluate the protective effects of vitamin C against CrVI toxicity. Specific Aim-2 will determine the effects of lactation exposure to CrVI on ovulation and oocyte quality in F1 offspring, and to evaluate the protective effects of vitamin C against CrVI toxicity. Specific Aim-3 will determine the effects of lactational exposure to CrVI on pregnancy outcomes in F1 female offspring and to evaluate the protective effects of vitamin C against CrVI toxicity. Successful completion of studies outlined in this project is expected to establish that: (i) CrVI will induce apoptosis of granulosa cells and follicular atresia, decrease oocyte quality, decrease ovulation and pregnancy rate, and induce preterm labor; and (ii) vitamin C will mitigate CrVI toxicity and protect the reproductive health of F1 offspring. Such results will have an important positive impact and are expected to have translational relevance for reproductive health in women and children, especially those working in industries or living in areas in which they are exposed to high levels of CrVI. In addition, the novel findings from this project are expected to provide a new knowledge on CrVI-induced ovotoxicity and infertility and protective effects of vitamin C, and that will fill the substantial gap in knowledge of CrVI-induced reproductive toxicity. This is an R21 application addressing the mission of NIH/NIEHS on environmental heavy metal toxicants and human health and NIH/NICHD on reproductive health of women and children.

描述（由申请人提供）：六价铬环境污染是对人类健康的主要威胁，并且由于铬的广泛工业用途而日益严重。在铬行业工作和生活在铬污染环境中的女性，会出现月经异常、不孕、死产等各种生殖问题，并伴随着血液和尿液中高铬含量。 CrVI 可通过母乳传递给新生儿。由于潜在的分子和细胞机制仍然很大程度上未知，因此尚未确定保护妇女生殖健康免受六价铬毒性不利影响的治疗干预策略。我们最近在大鼠中的研究结果表明，哺乳期暴露于 CrVI 会诱导卵泡闭锁和颗粒细胞凋亡，减少类固醇生成，延迟青春期并延长动情周期；而维生素 C 与 CrVI 一起补充可减轻 CrVI 的不利影响，恢复正常的卵泡发育和 F1 代青春期的开始。本申请的目的是了解哺乳期接触 CrVI 损害卵巢功能和妊娠的机制，并评估维生素 C 对 CrVI 诱导的 F1 后代生殖毒性的预防作用。我们的中心假设是，哺乳期暴露于 CrVI 会诱导颗粒细胞凋亡和卵泡闭锁，减少排卵，危及妊娠，并诱发 F1 雌性后代早产；维生素 C 可以减轻 CrVI 引起的生殖毒性。具体的 Aim-1 将确定哺乳期暴露于 CrVI 诱导 F1 后代卵巢滤泡闭锁的分子机制，并评估维生素 C 对 CrVI 毒性的保护作用。具体的 Aim-2 将确定哺乳期接触 CrVI 对 F1 后代排卵和卵母细胞质量的影响，并评估维生素 C 对 CrVI 毒性的保护作用。具体的 Aim-3 将确定哺乳期接触 CrVI 对 F1 雌性后代妊娠结局的影响，并评估维生素 C 对 CrVI 毒性的保护作用。本项目概述的研究的成功完成预计将确定： (i) CrVI 将诱导颗粒细胞凋亡和卵泡闭锁，降低卵母细胞质量，降低排卵和妊娠率，并诱发早产； (ii) 维生素 C 将减轻 CrVI 毒性并保护 F1 后代的生殖健康。这些结果将产生重要的积极影响，并有望对妇女和儿童的生殖健康产生转化意义，特别是那些在工业中工作或生活在高水平六价铬暴露地区的妇女和儿童。此外，该项目的新发现预计将为六价铬引起的卵毒性和不孕症以及维生素C的保护作用提供新的知识，这将填补六价铬引起的生殖毒性知识的巨大空白。这是一个 R21 应用程序，旨在履行 NIH/NIEHS 在环境重金属毒物和人类健康方面以及 NIH/NICHD 在妇女和儿童生殖健康方面的使命。

项目成果

Prenatal exposure to chromium induces early reproductive senescence by increasing germ cell apoptosis and advancing germ cell cyst breakdown in the F1 offspring.

产前接触铬会增加 F1 后代的生殖细胞凋亡和促进生殖细胞囊肿破裂，从而诱导早期生殖衰老。

• 发表时间: 2014-04-01
• 影响因子: 2.7
• 作者: Sivakumar, Kirthiram K;Stanley, Jone A;Arosh, Joe A;Pepling, Melissa E;Burghardt, Robert C;Banu, Sakhila K
• 通讯作者: Banu, Sakhila K