Enhanced Suveillance for New Vaccine Preventable Diseases

加强对新疫苗可预防疾病的监测

• 批准号: 8513799
• 负责人: Parvin H. Azimi
• 金额: $ 15.85万
• 依托单位: CHILDREN'S HOSPITAL & RES CTR AT OAKLAND
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8513799
• 关键词: Enhanced; Suveillance; New; Vaccine; Preventable

DESCRIPTION (provided by application): Active surveillance of acute gastroenteritis (AGE) will be conducted in the Emergency Dept. and in hospitalized patients who are seen at Children's Hospital & Research Center, Oakland (Oakland, CA), with the aims of determining the burden of AGE, the burden of rotavirus AGE, and the burden of norovirus AGE in children 15 days to <10 years of age. Clinical, demographic, and vaccination information will be collected for each patient. A stool sample will be collected from each patient within 14 days of onset of AGE symptoms. Stool samples will be tested on site by enzyme immunoassay (EIA), to detect rotaviruses. Stool samples will be tested for noroviruses by real-time polymerase chain reaction (RT-PCR). All stool samples demonstrating a positive result will be forwarded to CDC, for serotyping. Saliva samples will be obtained in a portion of AGE cases for determination of norovirus-secretor status. Age-matched children with acute respiratory infection (ARI) will be assigned for rotavirus cases, and age-matched normal children will be assigned for norovirus AGE cases. Population-based incidence rates by age will be determined for each of the following: total AGE, RV AGE and norovirus AGE, in hospitalized patients as well as patient seen only in the ED.

描述（由应用提供）：将在紧急部门以及在加利福尼亚州奥克兰（Oakland，CA）的儿童医院与研究中心（Oakland）中看到的急性胃肠炎（年龄）的积极监测，目的是确定年龄的负担，Rotavirus Ege的负担，Rotavirus Ege的负担，以及Norovirus的负担，以及Norovirus of Norovirus年龄15天。将为每位患者收集临床，人口统计和疫苗接种信息。年龄症状发作后的14天内将从每个患者那里收集粪便样本。粪便样品将通过酶免疫测定（EIA）在现场测试，以检测轮状病毒。粪便样品将通过实时聚合酶链反应（RT-PCR）测试北病毒。所有证明阳性结果的粪便样品将转发给CDC，用于血清分型。唾液样本将在年龄案例的一部分中获得，以确定诺如病毒 - 秘密状态。年龄匹配的患有急性呼吸道感染（ARI）的儿童将针对轮状病毒病例分配，并将年龄匹配的正常儿童分配给诺如病毒年龄病例。将针对以下每个年龄划分的基于人群的发病率：总年龄，RV年龄和诺如病毒年龄，住院的患者以及仅在ED中看到的患者。