Analysis of Cellular Plasticity in White Adipose Tissue

白色脂肪组织细胞可塑性分析

基本信息

批准号：
8370098
负责人：
James G Granneman
金额：
$ 33.06万
依托单位：
WAYNE STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-05-15 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8370098
关键词：
Abdomen Adipocytes Adipose tissue Adrenergic Agents Adrenergic Receptor Adult Alpha Cell Apoptosis Appearance Biogenesis Biological Models Cells Cellular Morphology Chronic Cues Development Diabetes Mellitus Diet Disease Dissection Fatty acid glycerol esters Goals Gold Human Image Imaging Techniques In Vitro Location Mediating Metabolic Metabolic Diseases Mitochondria Modeling Molecular Monitor Necrosis Obesity Phenotype Platelet-Derived Growth Factor Receptor Platelet-Derived Growth Factor alpha Receptor Process Proliferating Recruitment Activity Signal Transduction Stem cells Surface Techniques Testing Therapeutic Tissues adrenergic adult stem cell fatty acid oxidation feeding improved in vivo novel obesity treatment progenitor repaired response restoration self-renewal subcutaneous therapeutic target treatment strategy uncoupling protein 1

项目摘要

DESCRIPTION (provided by applicant): White adipose tissue (WAT) remodeling is an emerging therapeutic strategy for treatment of obesity-related disorders like diabetes. Remodeling of WAT by beta3 adrenergic activation is a robust model that allows mechanisms to be tested in vivo and in vitro. Using this model system, the progenitors of the brown adipocytes that appear in WAT were identified, and techniques for imaging, isolating, and genetically manipulating these cells in vivo were developed. These cells appear to act as adult 'remodeling stem cells' (rmSC) that are likely involved in the remodeling and cellular restoration of WAT under various conditions. This project will examine mechanisms by which these novel rmSC are recruited by WAT therapeutic remodeling and by chronic high fat feeding. The long term goal is to guide strategies for expanding metabolically desirable adipocyte phenotypes for treating obesity-related disorders. PUBLIC HEALTH RELEVANCE: This project will examine mechanisms by which novel adult stem cells in white adipose tissue are recruited by therapeutic remodeling and by chronic high fat feeding. The long term goal is to guide strategies for expanding metabolically desirable adipocyte phenotypes for treating obesity-related disorders.

描述（由申请人提供）：白色脂肪组织（WAT）重塑是一种治疗肥胖相关疾病（如糖尿病）的新兴治疗策略。通过beta3肾上腺素能激活对WAT进行重塑是一种可靠的模型，它允许在体内和体外测试机制。使用此模型系统，确定了出现在WAT中的棕色脂肪细胞的祖细胞，并开发了用于成像，分离和遗传操纵这些细胞体内这些细胞的技术。这些细胞似乎充当成人的“重塑干细胞”（RMSC），在各种条件下可能参与WAT的重塑和细胞恢复。该项目将检查这些新型RMSC通过WAT治疗重塑和慢性高脂肪进食来招募的机制。长期目标是指导策略，以扩大代谢性的脂肪细胞表型来治疗与肥胖相关的疾病。公共卫生相关性：该项目将检查通过治疗性重塑和慢性高脂肪进食来招募白色脂肪组织中新型成年干细胞的机制。长期目标是指导策略，以扩大代谢性的脂肪细胞表型来治疗与肥胖相关的疾病。