Development of a discovery platform based on microfluidics and fluorescent cell f

开发基于微流体和荧光细胞的发现平台

基本信息

批准号：
8331618
负责人：
BENJAMIN M BUEHRER
金额：
$ 33.91万
依托单位：
NIVARTA, INC.
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-09-15 至 2014-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8331618
关键词：
Adipocytes Adipose tissue Biological Assay Cell Culture System Cell Culture Techniques Cell Survival Cells Chemicals Development Devices Diabetes Mellitus Diagnostic Dimensions Disease Dose Drug Costs Fluorescence Genetic Transcription Glass Glucose Goals Growth Human Human Resources Image Insulin Label Libraries Liquid Chromatography Malignant Neoplasms Marketing Methods Microfluidics Microscope Molecular Obesity Patients Pharmaceutical Preparations Plasma Proteins Proteome Radioactive Radiolabeled Reagent Research Personnel Risk Robotics Safety Screening procedure Slide Technology Testing Therapeutic Time Tritium Tube Vial device base biochip cost cost effective culture plates design diabetes management glucose analog glucose uptake high throughput screening high throughput technology meetings nervous system disorder novel novel therapeutics radiotracer response small molecule small molecule libraries

项目摘要

DESCRIPTION (provided by applicant): The overall goal of the project is to develop discovery platform based on microfluidics and functional cell assays that is suitable for screening hundreds of proteins or small-molecule compounds simultaneously in a cost-effective and high throughput manner. The projects Specific Aims are: (1) To develop a microfluidic-based platform for high-throughput screening of potential protein and small molecule therapeutics. (2) To develop an insulin stimulated glucose uptake assay in differentiated 3T3-L1 adipocytes using fluorescent 2-deoxy glucose (2-DOG) analog. (3) To integrate and perform a functional 2-DOG uptake assay in the microfluidics based multi-well cell culture biochip. The deliverable from this completed project is a cost-effective microfluidics based platform suitable for high throughput screening of proteins and small molecule therapeutic compounds.

描述（由申请人提供）：该项目的总体目标是基于微流体和功能性细胞测定法开发发现平台，适用于以成本效益且高吞吐量的方式同时筛选数百种蛋白质或小分子化合物。该项目的特定目的是：（1）开发一个基于微流体的平台，用于对潜在蛋白质和小分子疗法的高通量筛查。（2）使用荧光2-脱氧葡萄糖（2-DOG）类似物在分化的3T3-L1脂肪细胞中开发胰岛素刺激的葡萄糖摄取测定。（3）在基于微流体的多孔细胞培养生物芯片中整合并执行功能性2-DOG摄取测定。该完成的项目的可交付是基于成本效益的微流体水平的平台，适用于蛋白质和小分子治疗化合物的高吞吐量筛选。