Molecular Mechanisms and Targets of Soy Compounds in the Prevention of Colon Canc

大豆化合物预防结肠癌的分子机制和靶点

基本信息

批准号：
8422792
负责人：
Zigang Dong
金额：
$ 16.58万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-01-01 至 2014-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8422792
关键词：
Asia Binding Biological Assay Bladder Breast Cancer Etiology Cell Culture Techniques Cessation of life Chemopreventive Agent Colon Colon Carcinoma Computer Simulation Computers Consumption Country Diet Docking Effectiveness Endometrial Epidemiologic Studies Esophageal Exhibits Food Genetic Goals Health Human Isoflavones Life Style Lung Malignant Neoplasms Methods Modeling Molecular Molecular Biology Molecular Target NIH Program Announcements Nutritional Obesity Observational Study Ovarian Pattern Physical activity Population Preclinical Drug Evaluation Prevention Prostate Protein Structure Databases Proteins Risk Safety Shapes Skin Stomach Technology Testing Thyroid Gland United States base bioactive food component cancer prevention cancer risk cancer therapy cancer type cellular targeting daidzein data management equol in vivo in vivo Model lifestyle factors models and simulation mouse model protein structure function public health relevance response screening soy soy protein isolate supercomputer

项目摘要

DESCRIPTION (provided by applicant): This R21 proposal is submitted in response to Program Announcement Number PA-10-088 entitled "Exploratory Cancer Prevention Studies Involving Molecular Targets for Bioactive Food Components." Consumption of soy based foods has been shown to exhibit varying degrees of inhibitory effects on numerous types of cancers. Colon cancer is a common cause of cancer death in the United States. Diet and lifestyle factors, including obesity and lack of physical activity, are believed to be associated with increased colon cancer risk. Numerous observational studies in humans have indicated that a high consumption of isoflavones or soy in certain countries is associated with a reduced risk of colon cancer. However, the evidence for positive health effects of the isoflavones in soy is quite inconsistent. Rigorous mechanistic studies are needed to elucidate the cellular targets and mechanisms of action of soy compounds against colon and other cancers. We propose to combine analyses of protein structure and function with advanced methods of data management and drug screening to identify potential target proteins for three isoflavone soy compounds. We will use supercomputer technology combined with molecular biology, cell culture models and an in vivo mouse model to test and validate the computer prediction of the molecular target(s) of daidzein and three of its metabolites.

描述（由申请人提供）：该R21提案是针对计划公告编号PA-10-088提交的，标题为“探索性癌症预防研究，涉及生物活性食品成分的分子靶标”。已经证明，基于大豆的食物的消费对多种类型的癌症表现出不同程度的抑制作用。结肠癌是美国癌症死亡的常见原因。饮食和生活方式因素，包括肥胖和缺乏体育锻炼，被认为与结肠癌的风险增加有关。在人类中进行的大量观察性研究表明，某些国家 /地区的异黄酮或大豆的大量消费与结肠癌的风险降低有关。但是，异黄酮在大豆中的健康影响的证据非常不一致。需要严格的机械研究来阐明大豆对结肠和其他癌症的细胞靶标和作用机制。我们建议将蛋白质结构和功能的分析与先进的数据管理和药物筛选方法相结合，以鉴定三种异黄酮大豆化合物的潜在靶蛋白。我们将使用超级计算机技术与分子生物学，细胞培养模型和体内小鼠模型相结合，以测试和验证大泽林及其三种代谢物的分子靶标的计算机预测。