Development of a Biomarker for Dietary Furanocoumarins

膳食呋喃香豆素生物标志物的开发

• 批准号: 8305071
• 负责人: Sabrina Trudo
• 金额: $ 14.9万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-24 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8305071
• 关键词: Address; Animal Model; Apiaceae; Area; Biological; Biological Assay; Biological Markers; Botanicals; CYP1A2 gene; CYP3A4 gene; Carcinogen Metabolism; Carcinogens; Carrots - dietary; Cause of Death; Characteristics; Chemopreventive Agent; Chemoprotective Agent; Chinese People; Citrus; Collaborations; Complement; Cytochrome P-450 CYP1A1; DNA Adducts; Data; Development; Diet; Diet Research; Dietary Practices; Dose; Drug Metabolic Detoxication; Eating; Edible Plants; Enzymes; Epidemiologic Studies; Epidemiology; Equilibrium; Excretory function; Exposure to; Faculty; Family; Food; Frequencies; Fruit; Furanocoumarins; Goals; Health; Hour; Human; In Vitro; Individual; Instruction; Intake; Intervention; Investigation; Isothiocyanates; Knowledge; Malignant Neoplasms; Measurement; Mediating; Metabolic; Metabolic Biotransformation; Methodology; Methods; Minnesota; Pattern; Phase; Phytochemical; Prevention Research; Principal Investigator; Questionnaires; Records; Reporting; Research; Research Activity; Resources; Rutaceae; Sampling; Second Primary Cancers; Singapore; Source; Spottings; Testing; Time; United States; Urine; Vegetables; Xenobiotics; anticancer research; base; bioactive food component; cancer prevention; cancer risk; carcinogenesis; career; cohort; cytochrome P-450 CYP2A6 (human); diet and cancer; feeding; fruits and vegetables; human data; interest; prospective; response; skills; tool; urinary

This project expects to develop the career of the candidate through didacticand research activities that complement the candidate's 5-year goal of studyingfuranocoumarins and cancer prevention in humansand 10- year goal ofstudying diet patterns and protection against cancer. Specifically, the career developmentplan includes the following objectives: 1) acquire knowledge and skillsin biomarker development,2) develop skills in epidemiologic study of diet and cancer prevention, and 3) build interdisciplinaryresearch associations and collaborations. The research plan will utilize the extensive resources and expertise at the Universityof Minnesota Masonic CancerCenter, an NCIdesignated ComprehensiveCancerCenter,to address two specific aims. The first aim is to develop a urinary biomarker of dietary exposure to furanocoumarins which are a group of compoundsthat may decrease cancer risk through inhibiting.activation of carcinogens. The second aim is to validate the biomarker for use in epidemiologicstudies to generate more human data in this area ofcancer prevention research. Senior faculty from the MasonicCancer Center will provide scientific and professional guidance in addition to the data and urine samples from a large prospective cohort in Singapore. First,we will modify existing methods for furanocoumarin analysis ofplants and various human biospecimens and develop a method for use with pooled human urine samples spiked with the analytes of interest. After determinationof the necessary conditionsand steps for optimal characterization of each analyte, we will chemically validate the method usingurine samples from 10 individualsgiven controlled amounts of furanocoumarin food sources and study excretion time and dose response. Lastly,usingdiet intake data (obtained by food frequeny questionnaire) and urine samples from the Singapore Chinese HealthStudy, we will validate the use of the urinary furanocoumarin biomarker with spot urine samples. This will includeanalysis of furanocoumarin food sources from Singaporeto calculate furanocoumarin intake based on the food frequencyquestionnaire data. RELEVANCE (See instructions): The results of this project will provide a much-needed tool for subsequent study of whether dietary furancoumarins can decrease the risk of cancer, the second leading cause of death in the United States. The project will also expand the number of dietary biomarkers available and the ability to assess whether various diet patterns protect against cancer.

该项目希望通过教义和研究活动发展候选人的职业 补充候选人在人类研究和预防癌症的5年目标和10- 研究饮食模式的年度目标和防止癌症的保护。具体而言，职业发展计划 包括以下目标：1）获取知识和技能，在生物标志物发展中，2）发展技能 饮食和癌症预防的流行病学研究，以及3）建立跨学科研究协会和 协作。该研究计划将利用大学的广泛资源和专业知识 明尼苏达州共济会cancercenter是一个综合综合的人，以解决两个特定的特定 目标。第一个目的是开发饮食中的尿生物标志物，暴露于Furanocoumarins上 化合物可能通过抑制降低癌症的风险。致癌物的激活。第二个目标是 验证在流行病学研究中使用的生物标志物，以在该领域的业务中生成更多的人类数据 预防研究。共济会中心的高级教师将提供科学和专业 除了来自新加坡大型前瞻性队列的数据和尿液样本外，指导。首先，我们会的 修改现有的植物和各种人类生物测量分析的方法分析的方法 与关注分析物相呼应的合并的人类尿液样品使用的方法。确定后 每个分析物的最佳表征的必要条件和步骤，我们将化学验证 使用来自10个个体受控数量的Furanocoumarin食物来源和 研究排泄时间和剂量反应。最后，使用丹特摄入数据（通过食物频率获得 问卷）和来自新加坡中国健康研究的尿液样本，我们将验证使用 尿液富诺康林生物标志物，带有斑点尿液样品。这将包括Furanocoumarin食物的分析 Singaporeto的来源根据食品频率汇总数据来计算Furanocoumarin的摄入量。 相关性（请参阅说明）： 该项目的结果将提供急需的工具，用于随后研究饮食 Furancoumarins可以降低癌症的风险，这是美国第二大死亡原因。 该项目还将扩大可用饮食生物标志物的数量以及评估是否能够评估的能力 各种饮食模式可以防止癌症。