Treatment of disc degeneration by nano-fullerenes

纳米富勒烯治疗椎间盘退变

• 批准号: 8309470
• 负责人: XUDONG J. LI
• 金额: $ 20.79万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8309470
• 关键词: Animal Experiments; Apoptosis; Apoptotic; Back; Back Pain; Biochemistry; Biological; Cell membrane; Cell-Free System; Cells; Chemical Structure; Chemicals; Chronic; Clinical; Environment; Excision; Exhibits; Extracellular Matrix; Fullerenes; Growth Factor; Half-Life; Human; Hydrogen Peroxide; In Vitro; Inhibition of Apoptosis; Interleukin-1; Interleukins; Intervertebral disc structure; Lead; Low Back Pain; Methods; Modality; Modeling; Mono-S; Nanostructures; Natural regeneration; Needles; Operative Surgical Procedures; Oryctolagus cuniculus; Outcome; Oxidative Stress; Pain; Physicians; Play; Preparation; Prevention; Process; Property; Proteins; Publishing; Puncture procedure; Reactive Oxygen Species; Reporting; Research; Role; Scheme; Science; Scientist; Societies; Solubility; Solutions; Staging; Supplementation; Surgeon; Suspension substance; Suspensions; Symptoms; Techniques; Therapeutic; Tissue Engineering; Tissues; Vertebral column; Yang; aqueous; base; disability; experience; fullerene C60; in vivo; intervertebral disk degeneration; nano; nanoparticle; nanostructured; novel; novel therapeutics; nucleus pulposus; professor; treatment strategy

DESCRIPTION (provided by applicant): Symptomatic intervertebral disc degeneration appears to be one of the most prevalent causes of chronic low back symptoms. Although therapeutic techniques including surgical removal and tissue engineering have been applied/proposed, the treatment of disc degeneration still remains a great challenge to both clinical physicians and basic scientists. Oxidative stress is regarded to play an important role in the early stages of disc degenerative process. However, therapeutic strategies using antioxidative agents have been ignored in the treatment of disc degeneration, and in particular, there are no reports on the effects of fullerene and its derivatives on disc degeneration published. The aim of the project is to characterize the inhibitory effects of aqueous solutions of a specific fullerene C60 derivative, bis- methanophosphonate fullerene (BMPF) on degenerative changes of intervertebral disc in vitro and in vivo based on the hypothesis that they might be very effective in the treatment of disc degeneration. Aqueous nanoparticle suspension will be prepared from fullerene and its derivatives including BMPF and C60, a mono- methanophosphonate fullerene, a fullerol and two carboxyfullerenes. Their protective activities against apoptosis and matrix destruction of cultured human disc cells induced by hydrogen peroxide or IL-1 will be investigated. Their inhibitory effects on disc degeneration will be further evaluated by using a rabbit annulus needle puncture model. The present study involving chemical, materials science and biomedicine will provide useful clues to the therapeutic strategy of disc degeneration and possibly lead to some novel potential candidates for the treatment of disc degeneration.

描述（由申请人提供）：有症状的椎间盘变性似乎是慢性下背部症状最普遍的原因之一。尽管已经应用/提出了包括手术清除和组织工程在内的治疗技术，但椎间盘退化的治疗仍然是临床医生和基础科学家的巨大挑战。氧化应激被认为在椎间盘退化过程的早期阶段起重要作用。但是，使用抗氧化剂的治疗策略在治疗椎间盘变性时已被忽略，特别是，尚无关于富勒烯及其衍生物对椎间盘变性的影响的报道。 该项目的目的是表征特定富勒烯C60衍生物的水溶液的抑制作用，基于体外椎间盘和体内椎间盘的退化变化，基于它们可能非常有效的椎间盘退化治疗，对椎间盘椎间盘的退化变化。纳米颗粒悬浮液将由富勒烯及其衍生物制备，包括BMPF和C60，一种单磷酸富勒烯，富勒罗和两个羧酸烯烯。他们的保护活性将研究由过氧化氢或IL-1诱导的培养的人盘细胞的细胞凋亡和基质破坏。通过使用兔环穿刺模型，将进一步评估它们对椎间盘变性的抑制作用。涉及化学，材料科学和生物医学的本研究将为椎间盘变性的治疗策略提供有用的线索，并可能导致一些新型的潜在候选椎间盘变性。

项目成果

Animal models for disc degeneration-an update.

• DOI: 10.14670/hh-11-910
• 发表时间: 2018-06
• 期刊: Histology and histopathology
• 影响因子: 2
• 作者: Jin L;Balian G;Li XJ
• 通讯作者: Li XJ

A novel culture platform for fast proliferation of human annulus fibrosus cells.

• DOI: 10.1007/s00441-016-2497-4
• 发表时间: 2017-02
• 期刊: Cell and tissue research
• 影响因子: 3.6
• 作者: Xiao L;Ding M;Saadoon O;Vess E;Fernandez A;Zhao P;Jin L;Li X
• 通讯作者: Li X

Antioxidative nanofullerol prevents intervertebral disk degeneration.

• DOI: 10.2147/ijn.s60853
• 发表时间: 2014
• 期刊: International journal of nanomedicine
• 影响因子: 8
• 作者: Yang X;Jin L;Yao L;Shen FH;Shimer AL;Li X
• 通讯作者: Li X

Fullerol nanoparticles suppress inflammatory response and adipogenesis of vertebral bone marrow stromal cells--a potential novel treatment for intervertebral disc degeneration.

• DOI: 10.1016/j.spinee.2013.04.004
• 发表时间: 2013-11
• 期刊: SPINE JOURNAL
• 影响因子: 4.5
• 作者: Liu, Qihai;Jin, Li;Shen, Francis H.;Balian, Gary;Li, Xudong Joshua
• 通讯作者: Li, Xudong Joshua

A New Formyl Peptide Receptor-1 Antagonist Conjugated Fullerene Nanoparticle for Targeted Treatment of Degenerative Disc Diseases.

• DOI: 10.1021/acsami.9b11783
• 发表时间: 2019-09
• 期刊: ACS applied materials & interfaces
• 影响因子: 9.5
• 作者: Li Xiao;Rong Huang;Yi Zhang;Tinghui Li;Jun Dai;Naga Nannapuneni;Timothy R. Chastanet;Matthew Chen;F. Shen;Li Jin;H. Dorn;X. Li