The Role of Keratinocytes as Independent Thermosensors

角质形成细胞作为独立热传感器的作用

• 批准号: 8322828
• 负责人: Jaime Garcia-Anoveros
• 金额: $ 5.05万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8322828
• 关键词: Address; Affect; Afferent Neurons; Alleles; Blood flow; Body Temperature; Calcium; Cations; Cell Line; Cells; Data; Development; Disease; Eccrine Glands; Epidermis; Epithelial; Epithelial Cells; Epithelium; Esthesia; Family; Genes; Growth; Hair; Hair follicle structure; Heating; Human; Image; Knockout Mice; Labyrinth; Lead; Mediating; Melanosomes; Messenger RNA; Morphology; Mus; Nervous system structure; Perception; Physiological; Pigmentation physiologic function; Production; Proliferating; Regulation; Research; Role; Sensory; Skin; Skin Temperature; Spinal Ganglia; Staging; Stratum Basale; Sweat; Sweating; Temperature; Temperature Sense; Testing; Time; Universities; Variant; cold temperature; keratinocyte; keratinocyte differentiation; mouse model; patch clamp; peripheral blood; receptor; recombinase; response; skin disorder; warm temperature

Thermosensitive channels in skin keratinocytes and/or in sensory neurons innervating the skin open in response to heating or cooling and mediate our perception of ambient temperature. We have found that some basal epidermal cells, as well as matrix keratinocytes of the hair follicles, express the TRPML3 channel. We have also observed that this channel opens in response to changes in temperature that go from cold towards the homeostatic temperature of skin. Here we propose to address the following questions. (1) Which epithelial cells express TRPML3 channels, and at which stages of epidermal and folliclular epithelial development do these express these cation channels? (2) Do these cells display cold-to-warm thermosensitive currents and are these currents mediated by their endogenous TRPML3 channels? (3) Does TRPML3 elimination affect hair follicle or epidermal morphology? These studies aim at elucidating the function ofTRPML3 channels in the epidermis and hair follicle, and may lead to further studies of how the skin, including the vasculature and eccrine glands, detect and respond to changes in temperature.

皮肤角质形成细胞和/或支配皮肤的感觉神经元中的热敏通道在 对加热或冷却的反应并调节我们对环境温度的感知。我们发现一些 基底表皮细胞以及毛囊的基质角质形成细胞表达 TRPML3 通道。我们有 还观察到，该通道的打开是为了响应从冷到冷的温度变化。 皮肤的稳态温度。在此，我们建议解决以下问题。 (1) 哪些上皮细胞 表达 TRPML3 通道，以及在表皮和滤泡上皮发育的哪个阶段进行这些表达 表达这些阳离子通道？ (2) 这些细胞是否表现出从冷到暖的热敏电流？ 由内源性 TRPML3 通道介导的电流？ (3) TRPML3消除是否影响毛囊或 表皮形态？这些研究旨在阐明 TRPML3 通道在表皮和 毛囊，并可能导致进一步研究皮肤（包括脉管系统和小汗腺）如何检测 并对温度的变化做出反应。