FLOW CYTOMETRY AND CELL SORTING

流式细胞术和细胞分选

• 批准号: 8436444
• 负责人: William L. Carroll
• 金额: $ 5.56万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-03-01 至 2018-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8436444
• 关键词: Basic Science; Cancer Center Support Grant; Cell Separation; Cells; Cellular biology; Clinic; Clinical; Cloning; Color; Communities; Consultations; Data; Dimensions; Equipment; Faculty; Flow Cytometry; Fluorescence; Growth; Hand; Hour; Human; Human Resources; Individual; Institutes; Institution; Instruction; Kaplan Cancer Center; Laboratories; Lasers; Malignant Neoplasms; Modality; Patient Care; Policies; Property; Research; Research Personnel; Resource Sharing; Resources; Running; Schedule; Services; Sorting - Cell Movement; Speed; Sterility; Students; System; Technical Expertise; Technology; Training; Training Activity; Training Technics; Validation; anticancer research; cancer therapy; cell type; design; instrument; light scattering; meetings; member; pre-doctoral; research study; virtual

PROJECT SUMMARY (See instructions): The Flow Cytometry & Cell Sorting Shared Resource is a critical technology for members of the NYU Cancer Institute (NYUCI). Flow Cytometry allows analysis of the light scattering and fluorescence properties of individual cells and the rapid, statistically detailed analysis of 10,000s of cells. The data on individual cells is retained, and subpopulations can be identified in multiple dimensions, showing distinct phenotypical cell types. Cells with particular fluorescence profiles can also be purified and collected for growth or further analysis using the cell sorting. The Flow Cytometry & Cell Sorting Shared Resource offers users who cannot afford and maintain their own Sorters and Analyzers access to this technology, as well as the technical expertise required to design experiments and evaluate experimental results. The facility has a Beckman Coulter MoFlo sorter with 4 lasers and up to 9 colors of Fluorescence, a Becton Dickinson 5-laser 14-color ARIA II cell sorter in a BSL-2 biosafety enclose for primary human cell sorting, an iCyt/SONY Reflection parallel cell sorter, as well as a 5-laser 17-color Becton Dickinson LSRII analyser. The shared resource also has a Becton Dickinson FACScalibur and FACScan analyzer. All cell sorting experiments are performed by highly-trained shared resource staff, and analyzers are run by investigators who have been trained by the shared resource staff. The scientific director also participate in training activities including individual consultations, public seminars and hands on training of users. The facility performed 3,887 hours of analysis and 3,111 hours of sorting during the most recent 12 month period.

项目摘要（请参阅说明）： 流式细胞仪和细胞分类共享资源是NYU癌症研究所（NYUCI）成员的关键技术。流式细胞仪可以分析单个细胞的光散射和荧光性能以及对10,000个细胞的快速，统计详细的分析。保留了单个细胞的数据，可以在多个维度上识别亚群，显示出不同的表型细胞类型。具有特定荧光特征的细胞也可以纯化并收集使用细胞分选的生长或进一步分析。流式细胞仪和细胞分类共享资源为无法负担和维护自己的分类器和分析仪访问该技术的用户以及设计实验并评估实验结果所需的技术专业知识。该设施有一个贝克曼·库尔特·莫夫洛（Beckman Coulter Moflo）分隔器，带有4个激光器和多达9种颜色的荧光，在BSL-2生物防护量中，用于原代人类细胞排序的BSL-2生物安全包装中的Becton Dickinson 5-laser 14彩色ARIA II细胞分隔器，一个冰冷/Sony Reflection-Reflection平行细胞混蛋，以及5- l las a 17-color becton。共享资源还具有Becton Dickinson Facscalibur和Facscan Analyzer。所有细胞分类实验均由受过良好训练的共享资源人员进行，分析仪由经共享资源人员培训的调查人员运行。科学主任还参加培训活动，包括个人咨询，公开研讨会和动手对用户的培训。该设施在最近的12个月内进行了3,887小时的分析和3,111小时的分类。