Microscopy

显微镜检查

基本信息

批准号：
8466735
负责人：
FRANK B GERTLER
金额：
$ 14.5万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8466735
关键词：
4D Imaging Address Animals Bioinformatics Biological Bioluminescence Biomedical Research Biophysics CCR Cancer Biology Cancer Center Cancer Center Support Grant Cancer Model Cells Cellular biology Collection Commit Confocal Microscopy Contract Services Contrast Sensitivity Core Facility Data Deposition Detection Development Diagnostic Discipline Doctor of Philosophy Electron Microscopy Ensure Equipment Faculty Fee-for-Service Plans Floor Fluorescence Fluorescence Microscopy Foundations Funding Goals Grant Hour Housing Image Image Analysis Imaging technology Immunoelectron Microscopy Institutes Intention Investigation Label Laboratories Lasers Life Light Malignant Neoplasms Microscope Microscopy Mission Molecular Mus Nanotechnology Noise Optics Preparation Published Comment Qualifying Reporter Research Research Personnel Resolution Resource Sharing Resources Review Committee Running Sampling Scanning Scientist Services Signal Transduction Spectral Karyotyping Speed System Techniques Technology Therapeutic Time Tissues Training Training Support Update Vacuum Wages animal tissue base cost design digital fluorescence imaging imaging modality in vivo innovation insight instrument instrumentation laser capture microdissection light microscopy luminescence member mouse model non-invasive system programs success tomography tool tumor

项目摘要

Research at the Koch Institute combines a wide spectrum of innovative programs designed to understand tumor development and develop new treatments and diagnostic strategies. Many of the approaches taken, in disciplines ranging from molecular and cell biological to analysis of mouse models and nanotechnology, rely on imaging. In 2005, using non-renewable institutional funds, the Koch Institute established a Microscopy Core Facility that makes centralized, state-of-the art imaging equipment available to Center investigators and other NCI-funded investigators at MIT. CCSG funds are requested to provide continuing support for this Core. The Microscopy Core currently offers a wide range of imaging platforms including standard light and epifluoresence to digital deconvolution, spinning disk confocal microscopy, and systems for spectral Karyotyping (SKY) and laser capture microdissection. The Core is run by a highly qualified Core Manager, who has extensive expertise in a broad range of microscopy platforms and applications including electron microscopy and live cell/organotypic fluorescent 4D imaging. The Core Manager oversees all instrumentation, provides state-of-the-art training to Center members in both sample preparation and use of the Core's equipment and also conducts specific microscopy techniques for Kl users on a fee-for-service basis. She also collaborates with the Applied Therapeutics & Whole Animal Imaging Core Facility, providing technical support and user training for the whole-animal imaging instrumentation including luminescence/fluorescence detection and microCT applications. The Microscopy Core has proven invaluable to a large fraction of the Kl faculty by allowing them to execute microscopy-dependent projects that were previously impossible given the expense of acquiring and maintaining imaging equipment. In the upcoming grant period, the Kl will continue to add new equipment to both the Microscopy and Applied Therapeutics & Whole Animal Imaging Core Facilities to further expanding their imaging capabilities. This expansion, as well as high demand for existing services, requires addition of a technical assistant to the Microscopy Core Facility staff. User chargebacks provide partial support the Microscopy Core, but the operational costs are currently underwritten by Institutional funds that will expire in June 2009. Thus, the requested CCSG support is critical for the continued success of this essential Microscopy Core.

科赫研究所的研究结合了广泛的创新项目，旨在了解肿瘤的发展并开发新的治疗和诊断策略。从分子和细胞生物学到小鼠模型分析和纳米技术等学科中所采用的许多方法都依赖于成像。 2005 年，科赫研究所利用不可再生的机构资金建立了显微镜核心设施，为中心研究人员和麻省理工学院的其他 NCI 资助的研究人员提供集中的、最先进的成像设备。请求 CCSG 资金为该核心提供持续支持。显微镜核心目前提供广泛的成像平台，包括标准光和落射荧光到数字解卷积、转盘共焦显微镜以及光谱核型分析 (SKY) 和激光捕获显微切割系统。该核心由高素质的核心经理负责管理，他在各种显微镜平台和应用方面拥有丰富的专业知识，包括电子显微镜和活细胞/器官荧光 4D 成像。核心经理负责监督所有仪器，为中心成员提供最先进的样品制备和核心设备使用培训，并在收费服务的基础上为 Kl 用户提供特定的显微镜技术。她还与应用治疗和整体动物成像核心设施合作，为整体动物成像仪器（包括发光/荧光检测和 microCT 应用）提供技术支持和用户培训。事实证明，显微镜核心对大部分吉隆坡教职人员来说是无价的，它使他们能够执行依赖于显微镜的项目，而这些项目以前由于获取和维护成像设备的费用而无法实现。在即将到来的资助期内，吉隆坡将继续为显微镜、应用治疗和整体动物成像核心设施添加新设备，以进一步扩大其成像能力。这种扩张以及对现有服务的高需求需要在显微镜核心设施人员中增加一名技术助理。用户退款为显微镜核心提供部分支持，但运营成本目前由机构资金承担，该资金将于 2009 年 6 月到期。因此，所请求的 CCSG 支持对于这一重要显微镜核心的持续成功至关重要。