Ultra-Low Background NIR Fluorophores for In Vivo Imaging and Image-Guided Surger

用于体内成像和图像引导手术的超低背景近红外荧光团

基本信息

批准号：
8889834
负责人：
John V Frangioni
金额：
$ 37.15万
依托单位：
CURADEL, LLC
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-08-01 至 2015-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8889834
关键词：
Ultra Low Background NIR Fluorophores

项目摘要

DESCRIPTION (provided by applicant): Near-infrared (NIR) fluorescence has the potential to revolutionize image-guided surgery. However, ideal fluorophores for in vivo, and eventually clinical, use have not yet been described. Under an NIH Bioengineering Research Partnership (BRP) grant, the PI's laboratory has developed a surgical imaging system that simultaneously, and in real-time, acquires two independent wavelengths of NIR fluorescence emission images along with color video images. The imaging system has already been translated to the clinic, and is being formally evaluated in three NIH-funded clinical trials. Nevertheless, the fundamental limitation to the future success of this technology is the development of NIR fluorophores that perform optimally in the body, and which can be made widely available to other academic researchers. To be clinically viable, the ideal NIR fluorophore requires certain optical properties, including excitation and emission H800 nm, and both a high extinction coefficient (5) and quantum yield (QY) in serum. However, the reason why existing NIR fluorophores perform so poorly in vivo has more to do with biodistribution and clearance. After IV injection, the ideal NIR fluorophore would rapidly equilibrate between the intravascular and extra vascular spaces and would be cleared efficiently via renal filtration. To date, every NIR fluorophore described in the literature suffers from two fundamental flaws: 1) hepatic clearance, which results in NIR fluorescence signal throughout the GI tract that persists for hours, and/or 2) non-specific background uptake in normal tissues, which typically persists for hours and results in a low signal-to-background ratio (SBR). This grant builds upon an observation we made two years ago using NIR fluorescent quantum dots (Choi et al., Nature Biotechnol. 2007; 25: 1165-70). Unexpectedly, and for reasons only partially understood, zwitterionic organic coatings resulted in extremely low non-specific tissue uptake, rapid renal clearance, and no serum protein binding. However, purely anionic or cationic coatings gave the opposite results. Based on these data, we began collaborating with Drs. Patonay and Strekowski at Georgia State University, leaders in the field of NIR fluorophore chemistry, to synthesize zwitterionic heptamethine indocyanine NIR fluorophores. The preliminary results, described herein, demonstrate that both non-targeted and tumor-targeted zwitterionic NIR fluorophores have remarkable optical and in vivo properties, including 800 nm fluorescence, high 5 and QY, rapid renal clearance, absence of protein binding, and ultra-low non-specific tissue uptake (i.e., background). The specific aims of this grant are focused on the synthesis of optimized zwitterionic NIR fluorophores for in vivo and surgical imaging, on validating their use as targeted diagnostic agents for prostate cancer, and for scale-up from analytical to preparative production. Completion of these aims will lay the foundation for future clinical testing during image-guided surgery. Importantly, we also present an intellectual property strategy that will permit free sharing of optimized NIR fluorophores within the academic community.

描述（由申请人提供）：近红外 (NIR) 荧光有可能彻底改变图像引导手术。然而，尚未描述用于体内和最终临床使用的理想荧光团。在 NIH 生物工程研究合作伙伴 (BRP) 的资助下，PI 的实验室开发了一种手术成像系统，该系统可以同时实时采集两个独立波长的近红外荧光发射图像以及彩色视频图像。该成像系统已经应用于临床，并正在 NIH 资助的三项临床试验中进行正式评估。然而，该技术未来成功的根本限制是近红外荧光团的开发，该荧光团在体内表现最佳，并且可以广泛供其他学术研究人员使用。为了在临床上可行，理想的近红外荧光团需要一定的光学特性，包括激发和发射 H800 nm，以及血清中的高消光系数 (5) 和量子产率 (QY)。然而，现有近红外荧光团在体内表现如此差的原因更多地与生物分布和清除有关。静脉注射后，理想的近红外荧光团将在血管内和血管外空间之间快速平衡，并通过肾过滤有效清除。迄今为止，文献中描述的每种 NIR 荧光团都存在两个基本缺陷：1) 肝脏清除，导致整个胃肠道中的 NIR 荧光信号持续数小时，和/或 2) 正常组织中的非特异性背景摄取，这种现象通常会持续数小时，并导致信号背景比 (SBR) 较低。这项资助建立在我们两年前使用近红外荧光量子点进行的观察的基础上（Choi 等人，Nature Biotechnol. 2007；25：1165-70）。出乎意料的是，由于仅部分了解的原因，两性离子有机涂层导致极低的非特异性组织摄取、快速的肾脏清除以及无血清蛋白结合。然而，纯阴离子或阳离子涂料给出了相反的结果。根据这些数据，我们开始与博士合作。佐治亚州立大学的 Patonay 和 Strekowski 是近红外荧光团化学领域的领导者，他们合成了两性离子七次甲基吲哚菁近红外荧光团。本文所述的初步结果表明，非靶向和肿瘤靶向两性离子 NIR 荧光团均具有显着的光学和体内特性，包括 800 nm 荧光、高 5 和 QY、快速肾清除、不存在蛋白质结合以及超非特异性组织摄取低（即背景）。这笔资助的具体目标集中于合成用于体内和手术成像的优化两性离子近红外荧光团，验证其作为前列腺癌靶向诊断剂的用途，以及从分析型生产扩大到制备型生产。这些目标的完成将为未来图像引导手术中的临床测试奠定基础。重要的是，我们还提出了一项知识产权策略，允许在学术界免费共享优化的近红外荧光团。

项目成果

期刊论文数量（20）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Nanoparticle assembly: building blocks for tumour delivery.

DOI：
10.1038/nnano.2014.7
发表时间：
2014-02
期刊：
Nature nanotechnology
影响因子：
38.3
作者：
通讯作者：

Cartilage-Specific Near-Infrared Fluorophores for Biomedical Imaging.

DOI：
10.1002/anie.201502287
发表时间：
2015-07-20
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
0
作者：
Hyun H;Owens EA;Wada H;Levitz A;Park G;Park MH;Frangioni JV;Henary M;Choi HS
通讯作者：
Choi HS

Central C-C Bonding Increases Optical and Chemical Stability of NIR Fluorophores.

DOI：
10.1039/c4ra11225c
发表时间：
2014-01-01
期刊：
RSC advances
影响因子：
3.9
作者：
Hyun H;Owens EA;Narayana L;Wada H;Gravier J;Bao K;Frangioni JV;Choi HS;Henary M
通讯作者：
Henary M

uPAR-targeted multimodal tracer for pre- and intraoperative imaging in cancer surgery.

DOI：
10.18632/oncotarget.3680
发表时间：
2015-06-10
期刊：
Oncotarget
影响因子：
0
作者：
Boonstra MC;van Driel PB;van Willigen DM;Stammes MA;Prevoo HA;Tummers QR;Mazar AP;Beekman FJ;Kuppen PJ;van de Velde CJ;Löwik CW;Frangioni JV;van Leeuwen FW;Sier CF;Vahrmeijer AL
通讯作者：
Vahrmeijer AL

Sentinel Lymph Node Mapping of Liver.