Diverse Roles of Reactive Oxygen Species and Inflammation in Vascular Disease

活性氧和炎症在血管疾病中的多种作用

• 批准号: 8507552
• 负责人: Kathy K Griendling
• 金额: $ 219.71万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-13 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8507552
• 关键词: Address; Adoptive Transfer; Advanced Glycosylation End Products; Agonist; Angiotensin II; Animal Model; Animals; Anti-Inflammatory Agents; Anti-inflammatory; Antihypertensive Agents; Antioxidants; Apolipoprotein E; Arterial Fatty Streak; Atherosclerosis; Attention; Binding; Biology; Blood Pressure; Blood Vessels; Cell Adhesion Molecules; Cell Culture Techniques; Cell Nucleus; Cell Survival; Cell physiology; Cells; Chronic; Commit; Confocal Microscopy; Data; Development; Diabetes Mellitus; Diabetic Angiopathies; Disease; Disease model; Dose; Down-Regulation; Endothelial Cells; Endothelium; Environment; Equilibrium; Event; Future; Gene Expression; Genetically Modified Animals; Genotype; Goals; Growth; Growth Factor; Health; Hindlimb; Histology; Home environment; Homing; Hormonal; Hormones; Hyperglycemia; Hypertension; Image; Immunization; Impairment; Infection; Inflammation; Inflammatory; Inflammatory Response; Injury; Interdisciplinary Study; Interleukin-17; Ischemia; Knockout Mice; Lead; Lesion; Link; Macrophage Activation; Matrix Metalloproteinases; Measurement; Measures; Mediating; Mediator of activation protein; Metabolic; Microscopy; Modeling; Molecular; Mus; NADPH Oxidase; Organism; Oxidative Stress; Pathology; Pathway interactions; Physiological; Platelet-Derived Growth Factor; Play; Predisposition; Process; Production; Qualifying; Reactive Oxygen Species; Receptor Cell; Regulation; Relaxation; Renal function; Research; Research Design; Research Personnel; Rodent; Role; Scientist; Signal Transduction; Signaling Molecule; Signaling Protein; Small Interfering RNA; Smooth Muscle Myocytes; Source; Stimulus; T-Cell Activation; T-Cell Receptor; T-Lymphocyte; Testing; Tissues; Translating; Tumor Necrosis Factor-alpha; Vascular Diseases; Work; abstracting; attenuation; base; bone morphogenetic protein receptor type II; bone morphogenetic protein receptors; bone morphogenic protein; cell growth; cell type; cytokine; diabetic; experience; hemodynamics; human TNF protein; improved; in vivo; inflammatory marker; insight; interest; macrophage; migration; monocyte; novel; oxidant stress; pathogen; programs; protective effect; protein function; receptor; repaired; response; response to injury; scaffold; shear stress; therapeutic target; vascular inflammation; vascular smooth muscle cell proliferation

DESCRIPTION (provided by applicant): For the past 17 years, investigators at Emory have been studying the sources, regulation and functional implications of reactive oxygen species (ROS) and inflammation in vascular biology and disease. Our PPG application builds on this expertise to test the overall hypothesis that ROS within the vessel wall lead to inflammatory processes that are central mediators of vascular disease. In project 1, Dr. David Harrison proposes that pre-activation of T-cells augments hypertension and will investigate the role of these cells in modulating vascular renal function. He will also study the role of IL-17 in mediating this effect, and test the antihypertensive potential of two therapies to inhibit T cell activation and homing. In project 2, Dr. Hanjoong Jo will investigate an exciting new hypothesis that loss of the bone morphogenic protein type II receptor (BMPRII), such as occurs in response to the inflammatory cytokine TNF-a, unleashes signaling proteins that are normally bound to the receptor and kept inactive, resulting in an uncontrolled activation of inflammatory pathways and the development of atherosclerosis. Dr. Jo will combine cell culture studies aimed at defining the mechanism underlying BMPRII regulation with studies using newly created, endothelial-specific BMPRlT'" ApoE'' mice to test these mechanisms in vivo. In project 3, Dr. W. Robert Taylor will examine the overall role of the receptor for advanced glycation end products (RAGE) in inhibiting collateral vessel formation in normal and diabetic conditions, focusing on the specific contributions of RAGE in monocytes and T cells, which are critical for the formation of collateral blood vessels. Additional studies will examine the ROS-dependent signaling to inflammatory gene expression, migration and cell viability. Project 4 will be directed by Dr. Kathy Grlendling, who proposes to study the differential roles of the NADPH oxidases Noxl and Nox4 in collateral formation and neointimal growth after vascular injury. This project includes studies designed to understand the mechanisms responsible for the opposite regulation of Noxl and Nox4, as well as the role of Nox4 in mediating the protective effects of BMP4. Dr. Grlendling will make use of Noxl and Nox4 knockout mice to investigate the function of these proteins in vivo. Four cores will support these projects. An administrative core, led by Dr. Grlendling, will provide administrative support for the program. A ROS core, led by Dr. Sergey Dikalov, will support state-of-the-art measurements of ROS, and a microscopy and histology core directed by Dr. Lula Hilenski will furnish expertise in confocal microscopy and imaging of inflammatory markers and ROS in cells and tissues. Finally, Dr. Bernard Lass^gue will lead an animal core to centralize rodent genotyping and husbandry. Overall, this research program will provide substantial new information defining the integrated mechanisms by which ROS and inflammation contribute to vascular disease. Ultimately, this research may establish new unifying concepts linking conditions that alter vascular oxidant stress and inflammation to the molecular processes underlying vasculopathies. (End of Abstract)

描述（由申请人提供）： 在过去的17年中，Emory的研究人员一直在研究活性氧（ROS）（ROS）和血管生物学和疾病中炎症的来源，调节和功能含义。我们的PPG应用以这种专业知识为基础，以检验总体假设，即血管壁内的ROS导致炎症过程是血管疾病的中心介体。在项目1中，戴维·哈里森（David Harrison）博士提出，预先激活T细胞会增加高血压，并将研究这些细胞在调节血管肾功能中的作用。他还将研究IL-17在介导这种作用中的作用，并测试两种疗法抑制T细胞激活和归巢的降压潜力。在项目2中，汉琼（Hanjoong Jo）博士将研究一个令人兴奋的新假设，即骨形态蛋白II型受体（BMPRII）的丧失，例如响应于炎性细胞因子TNF-A的响应，释放信号蛋白通常与受体绑定并保持不受机关，并导致不受控制的激活式的激活式的激活，并导致了无数的激活。 Jo博士将结合旨在定义BMPRII调节机制的细胞培养研究与使用新创建的，新的，特定于内皮的特定BMPRLT的研究，“ ApoE”小鼠在体内测试这些机制。关于单核细胞和T细胞的愤怒，这对于副血管的形成至关重要，这将检查ROS依赖的信号传导对炎症基因的表达，迁移和细胞生存能力。受伤。该项目包括旨在了解NOXL和NOX4相反调节的机制，以及NOX4在介导BMP4的保护作用中的作用。 Grlendling博士将利用NOXL和NOX4基因敲除小鼠研究这些蛋白质在体内的功能。四个核心将支持这些项目。由Grlendling博士领导的行政核心将为该计划提供行政支持。由Sergey Dikalov博士领导的ROS核心将支持ROS的最先进测量值，以及Lula Hilenski博士指导的显微镜和组织学核心将提供在共焦显微镜和细胞和组织中炎症标记和ROS的成像方面的专业知识。最后，伯纳德·拉斯（Bernard Lass）博士将领导动物核心，以集中啮齿动物的基因分型和饲养。总体而言，该研究计划将提供大量的新信息，以定义ROS和炎症导致血管疾病的综合机制。最终，这项研究可能会建立新的统一概念，以将血管氧化应激和炎症与血管病的分子过程改变。 （抽象的结尾）