Genetic Engineering Mouse Core

基因工程鼠标核心

基本信息

批准号：
8052127
负责人：
RONALD ANTHONY DEPINHO
金额：
$ 8.38万
依托单位：
DANA-FARBER CANCER INST
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-01-01 至 2011-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8052127
关键词：
Advanced Malignant Neoplasm Aging Alleles Animals Biological Markers Biological Models Biological Process Biology Cancer Biology Cancer Model Cells Chin Collaborations Communities Dominant-Negative Mutation Engineering Epigenetic Process Evolution Funding Gene Targeting Gene Transfer Techniques Generations Genes Genetic Genetic Engineering Genetically Engineered Mouse Goals Grant Human KRAS2 gene Knock-in Mouse Knock-out Knowledge Laboratories Laboratory mice Link MEKs Maintenance Malignant - descriptor Malignant Neoplasms Malignant neoplasm of pancreas Methods Molecular Mouse Strains Mus Mutation Oncogenic Pancreatic Ductal Adenocarcinoma Pathogenesis Pathology Pathology processes Pathway interactions Phosphotransferases Positioning Attribute Process Production Productivity Reagent Recording of previous events Research Research Personnel Role Science Services Signal Pathway Signaling Molecule Specialist Techniques Technology Tetanus Helper Peptide The Cancer Genome Atlas Training Transgenes Transgenic Mice Transgenic Organisms Uncertainty Ursidae Family Validation Work cell transformation design design and construction experience flexibility genetic manipulation host neoplasm interaction improved in vivo innovation insight meetings mouse genome mouse model new technology new therapeutic target novel tumor vector

项目摘要

The construction and phenotypic analysis of genetically engineered mouse (GEM) strains are fundamental and integral for the study of pancreatic cancer, including the analysis of signaling molecules in this pathology, the discovery and analysis of novel genes and their linked networks, and the validation and assessment of novel therapeutic targets and associated molecular biomarkers. The Genetic Engineering Mouse Core (GEMC) of this PDAC POI will provide all the necessary expertise, reagents and services to generate four genetically engineered mouse (GEM) modeling projects per year of grant funding. The GEMC will work closely with POI project leaders, investigators to produce the most advanced cancer relevant GEM strains. Specifically: (A) Transgenics; the GEMC will support all aspects of the construction of transgenic mouse models, including advice, service, technologies and reagents for the optimal design and construction of each specific transgene. (B) Gene Targeting; We will support all aspects of the construction of knockout and knock-in mouse alleles, including provide services, support, advice, technologies and reagents for the optimal design, construction and production of each specific targeting vector and resulting mice. (C) Evaluate and implement new technologies for the construction of genetically engineered mice and derivative cells.

基因工程小鼠 (GEM) 品系的构建和表型分析至关重要是胰腺癌研究的组成部分，包括信号分子的分析病理学、新基因及其相关网络的发现和分析，以及验证和评估新的治疗靶点和相关的分子生物标志物。基因工程该 PDAC POI 的鼠标核心 (GEMC) 将提供所有必要的专业知识、试剂和服务每年产生四个基因工程小鼠（GEM）建模项目。 GEMC 将与 POI 项目负责人、研究人员密切合作，制作最先进的癌症相关 GEM 菌株。具体来说： (A) 转基因； GEMC将支持转基因小鼠模型构建的各个方面，包括针对每个特定项目的优化设计和施工的建议、服务、技术和试剂转基因。 (B) 基因打靶；我们将支持敲除和敲入小鼠等位基因构建的各个方面，包括为优化设计、施工提供服务、支持、建议、技术和试剂以及每个特定靶向载体和所得小鼠的生产。 (C) 评估和实施构建基因工程小鼠的新技术衍生细胞。