Cell Culture Resource Core

细胞培养资源核心

• 批准号: 8059134
• 负责人: Yulia A Komarova
• 金额: $ 25.22万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8059134
• 关键词: Adenoviruses; B-Lymphocytes; Cell Culture Techniques; Core Facility; Endothelial Cells; Ensure; Genetic; Goals; Human; Knockout Mice; Lung; Methods; Mus; Permeability; Primary Cell Cultures; Principal Investigator; Proto-Oncogene Proteins c-akt; Quality Control; Recombinants; Reproducibility; Research Personnel; Resources; Schedule; Services; Signal Transduction; Techniques; Transfection; Transgenic Organisms; lung vascular injury; meetings; mouse model; programs; research study; success

Core B is a central resource for primary endothelial cell cultures and adenoviruses available for each of the four projects. This core facility will provide services for the following: 1) characterization and culture of primary human lung microvascular endothelial cells; 2) isolation, characterization and culture of primary lung microvascular endothelial cells of mouse origin including those of different genetic mouse models and 3) amplification and purification of recombinant adenoviruses for the experiments proposed in the Projects as outiined in the Core description below. Human and mouse lung endothelial cell cultures are pivotal for the accomplishment of studies proposed in all four Projects. Specifically, Core B is critical for providing endothelial cells isolated from different genetic mouse models; e.g.,fiklGPF*'"transgenic and Cav-1, eNOS, AKT-1, Src, Nox2 and PAR-1 knockout mice for

Core B 是原代内皮细胞培养物和腺病毒的核心资源，可用于每个 四个项目。该核心设施将提供以下服务：1) 初级特征和培养 人肺微血管内皮细胞； 2)原代肺的分离、表征和培养 小鼠来源的微血管内皮细胞，包括不同遗传小鼠模型的微血管内皮细胞和3) 重组腺病毒的扩增和纯化用于项目中提出的实验 下面的核心描述中概述了。人和小鼠肺内皮细胞培养对于 完成所有四个项目中提出的研究。具体来说，核心 B 对于提供 从不同基因小鼠模型中分离出的内皮细胞；例如，fiklGPF*'"转基因和 Cav-1、eNOS、 AKT-1、Src、Nox2 和 PAR-1 敲除小鼠