Imaging Core

成像核心

基本信息

批准号：
8518925
负责人：
SIMON C WATKINS
金额：
$ 2.21万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-05 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8518925
关键词：
Adoptive Transfer Animals Apoptosis Area Autoradiography Biological Biological Assay Biological Products Cell Communication Cell Death Cell Line Cells Clinical Collaborations Colorectal Cancer Complement Complementary DNA Computer Assisted Confocal Microscopy Consultations Data Documentation Electron Microscopy Electrons Fluorescence Fluorescence Microscopy Gene Expression Generations Genes Goals Hematopoietic Histology Human Image Image Analysis Imagery Imaging technology Immune Immune response Immune system Immunology Immunomodulators In Situ In Situ Hybridization In Vitro Individual Label Laboratories Lasers Life Light Location Luciferases Magnetic Resonance Imaging Malignant Glioma Malignant Neoplasms Measurement Messenger RNA Methods Microscopic Modeling Molecular Molecular Profiling Mus Natural Killer Cells Optical Methods Optical reporter Optics Pathology processes Pattern Play Process Productivity Proteins Publications Radioactive Reagent Reporter Genes Research Infrastructure Research Personnel Research Project Grants Resources Reverse Transcriptase Polymerase Chain Reaction Rodent Model Role Running Services Source Speed Staining method Stains T-Lymphocyte Techniques Technology Therapeutic Therapeutic Agents Time Tissues Training Transgenic Mice Transgenic Organisms Transmission Electron Microscopy Tumor Tissue Universities Vaccinia virus Viral Viral Genes base cRNA Probes cell killing cell motility cell type cellular imaging chemokine cost data sharing design experience image processing image visualization in vivo insight instrumentation light microscopy mRNA Expression melanoma molecular imaging neoplastic cell novel strategies optical imaging population based pre-clinical research programs protein expression research study response tool trafficking tumor tumor progression

项目摘要

The central goal of this program is to understand the therapeutic utility of novel strategies to stimulate Tumor cell killing by the immune system. The Imaging Core will play multiple diverse roles within this program. In summary, our primary roles will be : careful analysis of cellular and molecular processes associated with apoptosis, characterization and quantification of cell types within tumors, examination of protein expression during cell death, examination of cell-cell interactions in vitro, quantitative analysis of protein expression by individual cells within tissues, and measurement of the levels and patterns of expression of chemokines and cell-type markers in tumors and tumor explants. These studies will employ the full array of current light, and potentially, electron microscopic methods including: single and multicolor fluorescence microscopy, laser confocal microscopy, live cell imaging, transmission electron microscopy and computer-aided morphometric analyses. The Center for Biologic Imaging, in which this core (B1) service will be performed, is designed for the purpose of providing state of the art microscopic technologies to its users. It is equipped to perform a continuum of optical methods including all types of light and electron microscopy essential to this Program Project. Within the scope of this project at the light microscopic level these methods include: histology, immuno-histology, live cell and in situ hybridization methods. The Core will be used extensively by all projects. Core B2 will provide in situ analysis services to all projects, including: generation and sequence confirmation of gene-specific cDNA templates for probe synthesis; in situ hybridization with radioactive cRNA probes and autoradiography; combined in situ hybridization and immunohistochemical staining; and image capture, analysis, and documentation for data sharing and publication. These analysis services will provide integrated data on chemokine expression profiles and producer cells in tumor-containing tissues provided by all projects. Core B3, will act to provide whole animal MRI services as needed in each project. In the previous submission this core was very highly regarded as such changes have been kept to a minimum and only reflect continued interaction and productivity between the core and the project Pis RELEVANCE (Seeinstructions):

该计划的中心目标是了解刺激肿瘤的新策略的治疗效用免疫系统杀死细胞。成像核心将在该计划中发挥多种不同的作用。在总之，我们的主要作用是：仔细分析与相关的细胞和分子过程细胞凋亡、肿瘤内细胞类型的表征和定量、蛋白质表达的检查细胞死亡过程中，体外细胞间相互作用的检查，蛋白质表达的定量分析组织内的单个细胞，以及趋化因子和趋化因子表达水平和模式的测量肿瘤和肿瘤外植体中的细胞类型标记。这些研究将利用当前的全部光线，并且潜在的电子显微镜方法包括：单色和多色荧光显微镜、激光共焦显微镜、活细胞成像、透射电子显微镜和计算机辅助形态测量分析。将执行核心 (B1) 服务的生物成像中心专为为用户提供最先进的显微技术的目的。它被装备来执行光学方法的连续体，包括本计划所必需的所有类型的光学和电子显微镜项目。在该项目的光学显微镜水平范围内，这些方法包括：组织学、免疫组织学、活细胞和原位杂交方法。核心将被广泛使用项目。 Core B2将为所有项目提供原位分析服务，包括：生成和序列确认用于探针合成的基因特异性 cDNA 模板；放射性 cRNA 原位杂交探头和放射自显影；结合原位杂交和免疫组织化学染色；和图像用于数据共享和发布的捕获、分析和记录。这些分析服务将提供含肿瘤组织中趋化因子表达谱和产生细胞的综合数据所有项目。核心 B3 将根据每个项目的需要提供全动物 MRI 服务。在之前提交的该核心受到了高度重视，因为此类更改已保持在最低限度，并且仅反映核心和项目 Pi 之间的持续交互和生产力相关性（参见说明）：