Distribution correlation of P. gingivalis and S. cristatus in dental plaque

牙菌斑中 P. gingivalis 和 S. cristatus 的分布相关性

• 批准号: 8471686
• 负责人: HUA XIE
• 金额: $ 17.62万
• 依托单位: MEHARRY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-21 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8471686
• 关键词: Accounting; Actinomyces; Adult; Aerobic Bacteria; Affect; Africa; African American; American; Arginine deiminase; Bacteria; Caucasians; Caucasoid Race; Cells; Clinical; Code; Communicable Diseases; Communication; Complex; Dental Hygiene; Dental Plaque; Dental caries; Dentists; Development; Diabetes Mellitus; Disease; Environment; Exclusion Criteria; Exhibits; Female; Future; Genes; Genotype; Goals; Gram-Negative Anaerobic Bacteria; HIV; Health; Human; Incidence; Indigenous; Individual; Intervention; Laboratories; Lead; Learning; Medical History; Membrane Proteins; Microbial Biofilms; Modeling; Molecular; Not Hispanic or Latino; Odds Ratio; Oral; Oral cavity; Oxygen measurement, partial pressure, arterial; Pathogenesis; Patients; Periodontal Diseases; Periodontitis; Periodontium; Play; Population; Porphyromonas gingivalis; Predisposition; Pregnancy; Prevalence; Prevention; Production; Protein S; Protein Subunits; Proteins; Race; Recruitment Activity; Relative (related person); Reporting; Repression; Risk Factors; Role; Sampling; Signaling Molecule; Site; Streptococcus; Streptococcus cristatus; Streptococcus gordonii; Structure; Testing; Transcription Coactivator; Work; base; clinically relevant; design; fimbria; genetic regulatory protein; health disparity; male; microbial; microbial community; novel strategies; oral infection; oral pathogen; pathogen; periopathogen; prevent; social; tooth surface; treatment planning

DESCRIPTION (provided by applicant): Adult periodontitis is disproportionally distributed among races, and has a significantly higher incidence in non- Hispanic blacks compared to non-Hispanic whites, even when co-variates such as diabetes and other health and social factors have been taken into account. Titers to the oral pathogen P. gingivalis are reported to be higher, as anticipated, in dentate subjects with, compared to those without periodontitis. Our laboratory was to first to report an intergeneric communication between Streptococcus cristatus and P. gingivalis. We identified a surface protein of S. cristatus, arginine deiminase (ArcA), as a signal molecule to which P. gingivalis responds by repressing the expression of the fimA gene, which codes for the major subunit protein of long fimbriae. The latter is required for bacterial colonization of P. gingivalis and host cell invasion. Therefore this project will test the hypothess that: a) S. cristatus serves as a beneficial bacterium and plays an important role in the prevention of P. gingivalis colonization of the oral cavity; and b) that the increased incidence of periodontitis in African-Americans may be due to either a decreased ratio of oral S. cristatus: P. gingivalis in these subjects compared to whites, or that the FimA genotypes may differ in blacks vs. whites. Our aims therefore are to: 1) determine the distribution of S. cristatus and P. gingivalis in dental plaques of those with moderate periodontitis, severe periodontitis and periodontal healthy individuals, and compare microbial profiles (especially S. cristatus and P. gingivalis), in Africa Americans versus Caucasians within each group; and 2) to examine the correlation of these prevalence of S. cristatus and P. gingivalis with different FimA genotypes. To achieve these aims, we will recruit three groups of subjects: those with a healthy periodontium, or with moderate versus severe periodontitis. Each group will be composed of adult African American and Caucasian males and females. Exclusion criteria will include diabetes, HIV-AIDs and other health criteria such as pregnancy. All subjects will receive a full-mouth examination of periodontal status and a medical history. Microbial samples from dental plaque will be collected. The prevalence of S. cristatus and P. gingivalis will be determined by qPCR analysis, as will the prevalence of different FimA genotypes. We anticipate that the proposed studies will provide important information regarding the pathogenesis of periodontitis, and the microbial biofilms found in these two populations and will pave the way for extended studies in future, focused in the longer term on the development of appropriate interventional strategies targeting at eliminating of P. gingivalis colonization.

描述（由申请人提供）：成人牙周炎在种族之间分布不成比例，与非西班牙裔白人相比，非西班牙裔黑人的发病率明显更高，即使考虑了糖尿病和其他健康和社会因素等协变量考虑到。据报道，与未患牙周炎的受试者相比，患有牙周炎的有齿受试者的口腔病原体牙龈卟啉单胞菌效价更高，正如预期的那样。我们的实验室首先报告了冠状链球菌和牙龈卟啉单胞菌之间的属间通讯。我们鉴定了 S. cristatus 的表面蛋白精氨酸脱亚胺酶 (ArcA) 作为信号 牙龈卟啉单胞菌通过抑制 fimA 基因的表达来做出反应，该基因编码长菌毛的主要亚基蛋白。后者是牙龈卟啉单胞菌的细菌定植和宿主细胞入侵所必需的。因此，本项目将检验以下假设： a) 冠冠链球菌作为一种有益细菌，在预防牙龈卟啉单胞菌在口腔定植方面发挥着重要作用； b) 发病率增加 非裔美国人的牙周炎可能是由于与白人相比，这些受试者的口腔冠状疱疹：牙龈卟啉单胞菌的比例降低，或者黑人与白人的 FimA 基因型可能不同。因此，我们的目标是：1）确定中度牙周炎、重度牙周炎和牙周健康个体的牙菌斑中冠脉菌和牙龈卟啉单胞菌的分布，并比较微生物谱（特别是冠脉菌和牙龈卟啉单胞菌），每个群体中的非洲裔美国人与白种人； 2) 检查 S. cristatus 和 P. gingivalis 的患病率与不同 FimA 基因型的相关性。为了实现这些目标，我们将招募三组受试者：牙周组织健康的受试者，或患有中度或重度牙周炎的受试者。每个小组将由成年非裔美国人和白人男性和女性组成。排除标准包括糖尿病、艾滋病毒和其他健康标准，例如怀孕。所有受试者都将接受牙周状况和病史的全口检查。将收集牙菌斑中的微生物样本。 S. cristatus 和 P. gingivalis 的流行率以及不同 FimA 基因型的流行率将通过 qPCR 分析确定。我们预计，拟议的研究将提供有关牙周炎发病机制以及在这两个人群中发现的微生物生物膜的重要信息，并将为未来的扩展研究铺平道路，从长远来看，重点关注针对以下问题制定适当的干预策略：消除牙龈卟啉单胞菌定植。